We narrowed to 997 results for: lars;

...how a protein of interest actually behaves. How large is it? What parts of it move when you feed it substrate...does it have and how do they come off and on in a cellular environment? If these are pressing issues in your... proteins, lipid bilayers, organelles or other cellular impediments. FRET has been used in many applications...LanthaScreen) (Degorce et al., 2009), measuring intracellular calcium (Miyawaki, Griesbeck, Heim, & Tsien,...excellent primer on FRET and how to measure it). Molecular mailbox: How to get your fluorophore delivered...(a single mailbox at a specific house in your molecular city). However, the acceptor doesn’t need to go.... If the acceptor fluorophore is getting to a molecular mailbox that is close to the donor, faulty delivery...

...we developed assays to test large numbers of cancer variants across a large number of genes as systematically...structure. Sometimes this provides a great deal of clarity. But, often these methods don’t agree. Confusing...characterization For many years, we’ve hoped that large scale laboratory-based functional experiments that...poorly classified function and a well established cellular signaling gene, thus providing a starting point... us from testing this hypothesis: There was no large scale, broadly available collection of mutant cDNA...reference cDNA clones mapping to well-annotated cellular pathways). It wasn’t clear whether gene-agnostic...gene-agnostic bioassays could accurately test for molecular signatures of variant function and cancer phenotypes...

...transgene is incorporated into a large complex of both viral and cellular proteins and trafficked to the...Lentiviral vectors have been a staple in molecular biology for over three decades. Widely used across...This integrated viral genome is then part of the cellular DNA… forever!  This can be an advantage for researchers...enzymes that are proteolytically processed from the larger protein: PR (protease), RT (reverse transcriptase....  The envelope plasmid typically encodes the vesicular stomatitis virus G protein (VSV-G), which facilitates...proviral DNA is poised for integration, two types of circular episomes can be generated through either homologous... your own IDLVs is nearly identical to that of regular lentiviral vectors. The only difference is that...

...biology one often works with parts in the micromolar or nanomolar concentration range. Reactions to happen...of proteins. Split inteins are particularly useful for a kind of cellular computation, making possible ...Click Biology (Howarth, 2025). Figure 1: Modular assembly tools, alongside criteria for Click Biology...living systems. What are the possibilities for modular assembly using the reactive groups naturally present...defined as a set of reactions derived from the regular building-blocks of living cells, rapidly forming...fusion-tolerance — Click Biology tags should be similarly useful across the diverse kinds of proteins that...functionalities, such as fluorescence or triggering new cellular behavior like degradation or electrophile signaling...

...the end if you ever need to double-check your vocabulary! A brief review of base editing tools First, ...an inhibitor of base excision repair (BER), the cellular process that catches mutated U’s in DNA and ideally...Researchers in the Xueli Zhang lab were making similar observations around the same time. (The papers ... journal on the same day.) This team created a similar construct that they called a Glycosylase Base Editor...the base editor. Black arrows indicate natural cellular processes. Created with BioRender.com. Beyond...abasic site. But how that site is filled depends on cellular factors. Multiple labs tried to identify beneficial...repairing a GBE-derived abasic site requires more cellular factors binding to DNA. However, ABEs were improved...

...associated with cellular organelles, scientists have been able to study many cellular processes, including... for studying the organization and function of cellular systems. FPs have been used to track protein localization... localization, cell structure, intracellular trafficking, and protein turnover rates. Additionally, by...trafficking. Although FPs have enabled discovery of many cellular mechanisms, there are some limitations to working... ultimately misinterpretation of the protein’s cellular role. One way to avoid the pitfalls of overexpressed...SapTrap The Jorgensen Lab recently developed a modular plasmid assembly toolkit called SapTrap to improve... with C. elegans, several groups have designed modular toolkits to assist with tagging genomic loci in...

...antibodies may have similar emission spectra, meaning they will emit fluorescent light at similar wavelengths ...fluorescence for both at similar levels, meaning that your populations will ‘look’ similar on your flow plot....between the two of them because they emit at a similar wavelength. However, if your flow cytometer reads...is if the names of the fluorescent markers are similar, you will need to compensate between them (although...against each other, you should be able to draw perpendicular lines that intersect your populations. If you...population needs to be shifted, consider your perpendicular lines. In this case, the population on the left...needs to shift towards the X axis to become a perpendicular vertical line.     In general, most spillover...

... propensity of a particular type of molecule to form a dimer depends on its molecular affinity, termed...likely molecules of a particular type will interact. A protein with a nanomolar Kd will exist primarily...in cells while a protein with a high micromolar or low millimolar Kd is unlikely to form dimers in a cell... fused to a dimerizing FP has potential to form large stable polymers of dimers. 3) FRET biosensors that...dimerizing FP is fused to the POI. These structures are large (microns), bright, non-physiological, and difficult...the papers describing the FPs above including molecular sizing columns and native gels fail to detect ...vitro. Assays to detect FP dimerization in in vivo cellular contexts, such as the CytERM whorl formation assay...

...better access to intracellular targets. If your target is already on the extracellular side of the membrane...structure of your samples, the relationships between cellular components, and the accessibility of your target...cross-linkers work by (as the name suggests) cross-linking cellular proteins. Cross-linking is a chemical reaction...great for preserving your samples and maintaining cellular and tissue morphology. Formaldehyde is the most...ethanol, work by dehydrating and precipitating cellular components. This method of fixation is good for...Permeabilization What is the point?  Antibodies are large proteins that need a little help crossing cell membranes...is suitable for antibodies that bind to the extracellular portion of a protein. B) Permeabilizing with...

...) antibody-dependent cellular cytotoxicity (ADCC) c) antibody-dependent cellular phagocytosis (ADCP). ...die.  Antibody dependent cellular cytotoxicity (ADCC) Antibody dependent cellular cytotoxicity (ADCC) is... If the pathogen is a large parasite, ADCC can be induced through a very similar interaction between IgE...cytotoxins via degranulation.  Antibody-dependent cellular phagocytosis (ADCP) So far, our antibodies have...most famous for is eating. In antibody-dependent cellular phagocytosis (ADCP), Fcγ receptors on macrophages...modulators, shaping the immune response by driving particular cells or responses. And there are still antibody....2007.11.004 Xu Cao, et al. Promoting antibody-dependent cellular phagocytosis for effective macrophage-based cancer...