We narrowed to 728 results for: STO

1. Plasmids 101: Yeast Vectors

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   ...promote replication.  Selection markers for yeast Historically, scientists have utilized auxotrophic selection...

2. Kiran Musunuru on the Newest TALEN Genome-Editing System

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   ...modeling in stem cells. Addgene: Tell me about the history. How had investigators done this kind of work before...

3. Transferable Skills Guide: Cross-team Communication

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   ...service,” did he mean it the same way as Bryan, our customer support manager? When Gizela, the aforementioned...

4. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

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   ...rhodopsins, are highly constrained and poorly understood. It’s hard to design new channelrhodopsins with...

5. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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   ...at University of Texas Health Science Center at Houston. Prakash became interested in studying TS when ...

6. RNA Extraction Without A Kit

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   ...sample(s) are resuspended in water or TE buffer, store them in a -80°C freezer to prevent RNA degradation...

7. Antibodies 101: Selecting the Right Antibody

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   ...be variations within an application. Take immunohistochemistry (IHC), for instance. While an antibody may...

8. Transferable Skills Guide: Problem Solving

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   ...it is for helping your team reach an important milestone at work.   While I was in graduate school and ...

9. What's New in CRISPR - November 2021

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   ... within the FAH gene was precisely corrected, restoring expression in the liver.  Read the paper in Nature...

10. Antibodies 101: Polyclonal Antibodies

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    ...Fishman JB, Berg EA (2019) Antibody Purification and Storage. Cold Spring Harb Protoc 2019:pdb.top099101. https...

11. Viral Vectors 101: An Introduction to AAV

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    ...-based method for the titration of AAV-2 vector stocks."Molecular Therapy 6.2 (2002): 272. PubMed PMID...

12. CRISPRainbow and Genome Visualization

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    ...the 4D nucleome. Visualizing specific loci has historically been difficult, as techniques like fluorescent...

13. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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    ...ccdB accomplish this? Let’s start with a brief history of the gene and how molecular biologists have harnessed...

14. Visualizing Protein Turnover In Situ

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    ...science instructor at The Innovation Institute in Boston. When Eugenia is not by the microscope she likes...

15. All in a Twist: dsRNA

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    ... some exist as dsRNA. Still others use dsDNA to store their genetic information. All of these viruses ...

16. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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    ...dimerize, bringing each terminus of Cre together to restore Cre activity. As soon as red light or PCB is no...

17. Gendered Innovations: Why Does Sex of the Cell Matter?

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    ...blogger Londa Schiebinger, PhD, Hinds Professor of History of Science, Stanford University. Sex and gender...

18. Mouse Modeling, Part 2: Breeding and Crossing Mice

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    ...colony, as opposed to ordering control mice from a stock center. This serves as a better control since the...

19. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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    ...-CBP In this method, dCas9 is fused to CBP, an histone acetyltransferase domain capable of rearranging...

20. Corralling and Cornering the Goldilocks Antibody

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    ...compatible with the desired applications (such as immunohistochemistry (IHC) or western blot), and cost-effective...

21. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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    ... 2in1 Kit for FRET and ratiometric BiFC from Christopher Grefen’s lab or explore the MoBiFC modular bimolecular...

22. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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    ... in CDG patients are still poorly studied and understood.  In line with this, Prof. Videira and collaborators...

23. Educational Planarian Tools for Teaching Developmental Biology Techniques

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    ..., feedback etc. please write to cuttingclass@stowers.org and if you make new plasmids for investigating...

24. Lab Automation at Addgene

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    ...biggest benefits of the Biomek FxP is it’s customizability. It has a large deck that can house many different...

25. Progress Towards a PAM-Free CRISPR

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    ...Engineering a CRISPR Variant with PAM Flexibility History of CRISPR Cas – A Tale of Survival and Evolution...

26. Hot Plasmids February 2024

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    ... inspire further-improved versions. Thanks to Christophe Leterrier, whose FocalPlanes piece on mStayGold...

27. High Complexity Golden Gate Assembly with NEB’s DAD

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    ...already-existing sets. Note that this tool relies on a stochastic search algorithm rather than pre-designed sets...

28. Antibodies 101: Stripping and Reprobing Western Blots

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    ...applied antibodies and need to dry the membrane for storage, but plan to reprobe it later, strip it thoroughly...

29. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    ...plasmid that should be used to maintain a glycerol stock. Choose from: ampicillin, apramycin, blasticidin...

30. Antibodies 101: Choosing the Right Isotype

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    ...Antibody to Mouse Tissue Sections. In Journal of Histochemistry & Cytochemistry (Vol. 46, Issue 8, pp. ...