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  1. Editor's Choice, August 2016

    Type
    Blog Post
    ...Nature. Our take home: users likely need more time to optimize and troubleshoot the system. Site Directed...

  2. Scientist Networking: What is an Informational Interview?

    Type
    Blog Post
    ... and more open to developing a relationship. The time to develop relationships is before you need them...that is helpful. Keep in mind that this is a good time to ask personal questions about salary, opportunities...

  5. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ...published in the 1990s, biologists have spent a lot of time making things glow. Chances are you’ve used a GFP...already been made, and it would be expensive and time-consuming to repeat the process with these new photoconvertible...

  8. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ...the host plant genome. Scientists have worked to optimize this system for gene transfer, studying the stability...switching the E. coli origin of replication and codon-optimizing the Agrobacterium ORI, they achieved much higher...

  9. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...be crucial for a successful application. It takes time to prepare an excellent resume…it is worth it. Read...connections to the hiring group.  Read here for more on timely submission of a job application. 3) Read job descriptions...

  10. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    ...  Dr. Bourne strongly believes this is an amazing time of promise for science—we have powerful tools, beautiful...beautiful questions, and smart scientists.  At the same time, he pointed out, academic research has some big ...

  11. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ... we can’t localize one cell at a depth of one centimeter with fluorescence is because the light becomes...relevant papers, and then read them again. Most of the time, the answers will be there.     References: Bright...

  14. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...base editor pCMV-BE3 has been requested nearly 400 times, and newcomer pCMV-ABE7.10 has already earned a ...for Bacterial Genome Engineering and CRISPR Antimicrobials Day 7: Multiplexing and Cas9 variants: CRISPR...

  18. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...mice to gather the following types of data: Report time-course and concentration of released DA in mouse... dynamics in response to a threatening looming stimulus in vivo in transgenic zebrafish Report DA release...

  19. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...antibodies in llamas and alpacas (Figure 1). At the time, researchers were already on a quest for smaller...name.  We’ve talked about sdAbs a number of times on this blog over the years and have described how...

  21. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...monitor enzymatic activity or phosphorylation over time. You can also use biosensors to follow processes...Use to track a process (e.g. enzyme activity) over time or under various conditions Localization within...

  22. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ... purification is still possible but requires optimization and often more stringent washing. Nonetheless...protein purification, just as affinity tags can sometimes be repurposed for visualization. However, due ...

  23. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    ...cancerous, hybridoma, etc.), as well as sex and age at time of sampling if available. STR profiling for human...who got it from who knows where), now is as good a time as ever to find out the true identity of the line...

  24. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ...vector purification, and, most importantly, excessive time requirements. FastCloning is a simpler yet reliable...place for an hour at 37°C. This should be enough time to completely digest the template DNA. Note—templates...

  25. Management for Scientists: Giving Feedback

    Type
    Blog Post
    ...to become more effective and successful. Giving timely and useful feedback is an absolutely required aspect...this format and it becomes even more effective over time if you take it seriously.  Person who will receive...
Showing: 571 - 600 of 925 results