We narrowed to 931 results for: TIM

...gain access to an internship on the journey to full-time employment. I have always been interested in the...relating to their roles at Addgene. I had a great time speaking with Quintin Marcelino who is Addgene’s...

...Nature. Our take home: users likely need more time to optimize and troubleshoot the system. Site Directed...

... and more open to developing a relationship. The time to develop relationships is before you need them...that is helpful. Keep in mind that this is a good time to ask personal questions about salary, opportunities...

...play a role in the progression of cancer are often time consuming and expensive, especially when done in...functionally validated. In vitro assay allows first-time functional annotation of thousands of mutations ...

...infect only one or a few species of bacteria and sometimes, only a subset of strains of the same bacterial...researchers in the directory are volunteering their time and resources and there is a chance that an early...

...published in the 1990s, biologists have spent a lot of time making things glow. Chances are you’ve used a GFP...already been made, and it would be expensive and time-consuming to repeat the process with these new photoconvertible...

...what they need - whether it's their first or 500th time on addgene.org. Additionally we also wanted to highlight...look and explore the new homepage. We hope every time you come to Addgene to find the plasmid or viral...

...set of neurons can then be targeted with optical stimuli. The restricted light exposure to a specific area...opsin expression in their cell bodies. Optical stimulation in a different brain area can then reveal the...

...the host plant genome. Scientists have worked to optimize this system for gene transfer, studying the stability...switching the E. coli origin of replication and codon-optimizing the Agrobacterium ORI, they achieved much higher...

...be crucial for a successful application. It takes time to prepare an excellent resume…it is worth it. Read...connections to the hiring group.  Read here for more on timely submission of a job application. 3) Read job descriptions...

...  Dr. Bourne strongly believes this is an amazing time of promise for science—we have powerful tools, beautiful...beautiful questions, and smart scientists.  At the same time, he pointed out, academic research has some big ...

... we can’t localize one cell at a depth of one centimeter with fluorescence is because the light becomes...relevant papers, and then read them again. Most of the time, the answers will be there.     References: Bright...

...kanamycin resistance cassette will grow. However, often times, the plasmid backbone includes an antibiotic resistance...alleviates its toxic effect. Negative selection is sometimes called counterselection.  Toxin-antitoxin systems...

...mice to gather the following types of data: Report time-course and concentration of released DA in mouse... dynamics in response to a threatening looming stimulus in vivo in transgenic zebrafish Report DA release...

... still what I did as an SME, and then 75% of the time I’m kind of the go-to person for my department.”...department, and operations department with the ultimate goal of determining if projects are feasible. ...

...Hans uses for his job. “I am really more of a multimedia science communicator than a science writer these...support them. Many thanks to Hans for taking the time to speak with us! Follow Hans on Twitter @HansPacker...

...plants and that produce compounds with various antimicrobial activities (1). She has developed a fast endophyte...Improved Performance for Ratiometric and Fluorescence Lifetime Imaging” ACS Chemical Biology 2013 Mar 15;8(3)...

...monitor enzymatic activity or phosphorylation over time. You can also use biosensors to follow processes...Use to track a process (e.g. enzyme activity) over time or under various conditions Localization within...

...fluorophores and luciferases to track bacteria in real time, but each of these reporters has its drawbacks. ...bacteria, Bourdeau et al created two constructs optimized for expression in E. coli: acoustic gene 1 (arg1...

...making a custom feature in Snapgene. This saves us time as we are often analyzing deposits with many plasmids... to quickly align multiple sequences at the same time. This means we can easily compare and analyze any...

...vector purification, and, most importantly, excessive time requirements. FastCloning is a simpler yet reliable...place for an hour at 37°C. This should be enough time to completely digest the template DNA. Note—templates...

...antibodies in llamas and alpacas (Figure 1). At the time, researchers were already on a quest for smaller...name.  We’ve talked about sdAbs a number of times on this blog over the years and have described how...

... purification is still possible but requires optimization and often more stringent washing. Nonetheless...protein purification, just as affinity tags can sometimes be repurposed for visualization. However, due ...

...cancerous, hybridoma, etc.), as well as sex and age at time of sampling if available. STR profiling for human...who got it from who knows where), now is as good a time as ever to find out the true identity of the line...

...base editor pCMV-BE3 has been requested nearly 400 times, and newcomer pCMV-ABE7.10 has already earned a ...for Bacterial Genome Engineering and CRISPR Antimicrobials Day 7: Multiplexing and Cas9 variants: CRISPR...

...to become more effective and successful. Giving timely and useful feedback is an absolutely required aspect...this format and it becomes even more effective over time if you take it seriously.  Person who will receive...

... or yellow fluorescent protein at the time. It is 1.5 to 3 times brighter than the most commonly used ...

... be spending less time and resources resupplying our current inventory and more time providing new materials...

...genome editing toolkit. However, a challenge in optimizing base editing technologies across several cell... APOBEC- and Cas9-mediated Editing (ACE) in real-time. The reporter constitutively expresses eGFP to indicate...

...individuals interested in ordering from ABRC for the first time, we recommend reaching out to us at [email protected] Arabidopsis to your class for the first time – we want to hear from you. Reach out to us at ABRCedu...