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  1. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...cleaving and rejoining supercoiled DNA ends to facilitate replication. The TOPO technique specifically ...Site Directed Mutagenesis by PCR Use REPLACR Mutatgenesis to Easily Generate Insertion and Deletions in...

  5. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...Additionally, they have an IRES-Puro marker to facilitate stable cell clone selection. Expression is driven...QBI UCSF. They are particularly interested in facilitating collaborative science, and making it accessible...

  10. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...However, the first two generations of iGluSnFR had limitations: they were not optimal for experiments requiring...photon imaging.   Figure 1: 1P and 2P excitation and emission spectra of SF-Venus-iGluSnFR.A184V...

  11. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...translated in a specific compartment. Despite these limitations, these techniques represent powerful tools for...translation in various cell types and physiological states. References 1. Ingolia, Nicholas T., et al. “Genome-wide...

  13. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...below. Normalization is a fundamental and often understated problem in multiplex NGS library preparation....seqWell, Inc. He is a sequencing technologist and computational biologist with a passion for solving critical...

  14. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ...under CC BY from Mattei et.al., 2022.   Limitations of the in silico approach The in silico approach... verification. All approaches have their own limitations. Using methods together can inform decisions ...

  15. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...makes it easier to spot even minor weakness or limitations in a piece of research (Drummond & Fischhoff,... the National Academy of Sciences of the United States of America, 114(36), 9587-9592. doi:10.1073/pnas...

  16. How to Lead a Great Meeting

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    Blog Post
    ... the purpose of the meeting: Begin meetings by stating “The purpose of this meeting is to _________." ...ambassador” from each affected team to be the representative in the meeting and then communicate outcomes...

  17. Components of CRISPR/Cas9

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    Blog Post
    ...LbCpf1 RR variant 5' TYCV AsCpf1 RVR variant 5' TATV Campylobacter jejuni (CJ) 3' NNNNRYAC  Neisseria... as a scaffold linking the crRNA to Cas9 and facilitates processing of mature crRNAs from pre-crRNAs derived...

  18. Synthetic Photobiology: Optogenetics for E. coli

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    Blog Post
    ... image, as well as a genetic method for the computational problem of edge detection. Tabor et al. subsequently...some promoter activity persisted in the inactive state. The dynamic range, defined as the difference between...

  19. Fluorescent Proteins 101: Luciferase

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    Blog Post
    ...slower than fluorescent-based methods, such as excitation of Green Fluorescent Protein (GFP) because of...enhancers and untranslated regions, or the effect of mutations of these regulatory elements on gene expression...

  20. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ...Potential graduate students typically go through a rotation, which reveals their working habits, personality...the PI on any paper. Or perhaps, the lab has a reputation for not entering collaborations. Alternatively...

  21. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...non-functional 𝛽-galactosidase. Complementing this mutation by expressing this deleted portion, for example...custom primers are especially helpful in verifying mutations that wouldn't be reached by sequencing from either...

  22. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...plasmid of all time, but was found to have a point mutation in the puromycin cassette that makes it less efficient...(BB)-2A-Puro (PX459) V2.0. It contains a point mutation in the puromycin cassette that makes it less efficient...

  23. AAV Q&A with Tim Miles

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    Blog Post
    ...If you missed the webinar, you can watch the presentation and Q&A session on our YouTube channel. And ... a standard curve and somewhat more reliable quantitation. Differences in sample treatment across methods...

  24. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ... to use, not sensitive to light in its inactive state, and cheap. Unless there’s a specific reason you...a specific wavelength of light in response to excitation by a laser, which is then read by a detector ...

  25. Viral Vectors 101: Biosensors

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    Blog Post
    ...DNA, etc. The receptors job is to generate a quantitative signal upon recognizing the analyte. This signal...biosensor is the signal transducer which will quantitatively give a readout for whatever the bio-recognition...

  26. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...have a major impact on experimental design and interpretation of results. Take extra care when evaluating... present at both the injection site and in the putative projection neurons. When using this serotype to...

  30. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    ... are positive for your marker would be a good statistic to compare between samples. In cases where there...characterize your samples may allow for better interpretation. Priyamvada Prathima has a research background...
Showing: 571 - 600 of 836 results