We narrowed to 844 results for: Tat

...cleaving and rejoining supercoiled DNA ends to facilitate replication. The TOPO technique specifically ...Site Directed Mutagenesis by PCR Use REPLACR Mutatgenesis to Easily Generate Insertion and Deletions in...

... the National Academy of Sciences of the United States of America 110.39 (2013): 15521–15529. PMC. PubMed... the National Academy of Sciences of the United States of America 70.11 (1973): 3240–3244. PubMed PMID...

...requires very little hands-on labor and could facilitate the tagging of every protein in the C. elegans...other genome modifications, including targeted mutations. SEC-based systems may prove effective in other...

...translated in a specific compartment. Despite these limitations, these techniques represent powerful tools for...translation in various cell types and physiological states. References 1. Ingolia, Nicholas T., et al. “Genome-wide...

...later optimized these first generation FLiPs by mutating Dronpa to lower its aggregation tendency, increase...graduate student in Thomas J. Mansell’s lab at Iowa State University where she is developing fluorescent protein-based...

...high contrast between its resting state and its calcium-bound state, which was achieved by lowering the...

...cellular components ultimately leading to cell fragmentation and death (Elmore, 2007). To study apoptosis...fluorescence based reporters however have several limitations. First, FRET signals are often difficult to detect...

...below. Normalization is a fundamental and often understated problem in multiplex NGS library preparation....seqWell, Inc. He is a sequencing technologist and computational biologist with a passion for solving critical...

...reproducibility of experiments. Additionally, proper citation of these Addgene IDs in published work allows ... on the Addgene Blog Read a blog post on Our Citations Feature Check out this post on Scientific Reproducibility...

...makes it easier to spot even minor weakness or limitations in a piece of research (Drummond & Fischhoff,... the National Academy of Sciences of the United States of America, 114(36), 9587-9592. doi:10.1073/pnas...

...under CC BY from Mattei et.al., 2022.   Limitations of the in silico approach The in silico approach... verification. All approaches have their own limitations. Using methods together can inform decisions ...

...Society for Microbiology (2005). https://asm.org/getattachment/5c95a063-326b-4b2f-98ce-001de9a5ece3/gram-stain-protocol...

...However, the first two generations of iGluSnFR had limitations: they were not optimal for experiments requiring...photon imaging.   Figure 1: 1P and 2P excitation and emission spectra of SF-Venus-iGluSnFR.A184V...

...repository provides. Repositories around the world facilitate the rapid pace of SARS-CoV-2 research by removing...and SARS-CoV with implications for clinical manifestations, transmissibility, and laboratory studies of...

...Additionally, they have an IRES-Puro marker to facilitate stable cell clone selection. Expression is driven...QBI UCSF. They are particularly interested in facilitating collaborative science, and making it accessible...

... the purpose of the meeting: Begin meetings by stating “The purpose of this meeting is to _________." ...ambassador” from each affected team to be the representative in the meeting and then communicate outcomes...

...LbCpf1 RR variant 5' TYCV AsCpf1 RVR variant 5' TATV Campylobacter jejuni (CJ) 3' NNNNRYAC  Neisseria... as a scaffold linking the crRNA to Cas9 and facilitates processing of mature crRNAs from pre-crRNAs derived...

... image, as well as a genetic method for the computational problem of edge detection. Tabor et al. subsequently...some promoter activity persisted in the inactive state. The dynamic range, defined as the difference between...

...Potential graduate students typically go through a rotation, which reveals their working habits, personality...the PI on any paper. Or perhaps, the lab has a reputation for not entering collaborations. Alternatively...

...screens that looked for a CRISPR repeat array near a putative effector nuclease. The Cas13d proteins they identified...targeting the MAPT exon 10 splice acceptor and two putative splice enhancers. After AAV-mediated delivery ...

...BioBuilder curriculum is now taught in almost every state in the U.S. and in more than 30 countries around...The BioBuilder Learning Lab is a fully equipped, state-of-the-art lab embedded in LabCentral’s community...

...weeks of waiting, your flies will be ready for experimentation. Drosophila are amenable to many different ...PMCID: PMC1526508. 2. H. Jiang,  et al. Cytokine/Jak/Stat signaling mediates regeneration and homeostasis ...

...determine brightness are the extent to which the excitation light is absorbed and the efficiency by which...account, including the specs of your microscope (excitation wavelength, available emission filters, and detector...

...plasmid of all time, but was found to have a point mutation in the puromycin cassette that makes it less efficient...(BB)-2A-Puro (PX459) V2.0. It contains a point mutation in the puromycin cassette that makes it less efficient...

...SLIC compare to other cloning methods? SLIC’s limitations arise from its dependence on single-stranded ...may be assembled incorrectly. To overcome this limitation, one option is to perform a hierarchical assembly...

... are positive for your marker would be a good statistic to compare between samples. In cases where there...characterize your samples may allow for better interpretation. Priyamvada Prathima has a research background...

...DNA, etc. The receptors job is to generate a quantitative signal upon recognizing the analyte. This signal...biosensor is the signal transducer which will quantitatively give a readout for whatever the bio-recognition...

...have a major impact on experimental design and interpretation of results. Take extra care when evaluating... present at both the injection site and in the putative projection neurons. When using this serotype to...

...If you missed the webinar, you can watch the presentation and Q&A session on our YouTube channel. And ... a standard curve and somewhat more reliable quantitation. Differences in sample treatment across methods...

... to use, not sensitive to light in its inactive state, and cheap. Unless there’s a specific reason you...a specific wavelength of light in response to excitation by a laser, which is then read by a detector ...