We narrowed to 1,054 results for: STI

...control over when and where the enzyme is active, drastically reducing background labeling caused by the biotin...that had been trafficked from the “source” to “destination”. Images adapted from Lee and Cheah et al. 2023...roles in synapse development, transmission, and plasticity and is a popular marker of excitatory synapses...

...plasmid due to improper plasmid partitioning, it will still inherit some toxin and antitoxin. However, the antitoxin... serves as a counterselectable marker in the destination vector. Successful cloning occurs when the ccdB...to apply this system as an orally administered diagnostic that can indicate the presence of liver metastasis...

...faster, and more efficient than previous methods. Still, generating reliable results from CRISPR edit data...comparing the robustness, accuracy, and speed with existing tools. For example, we examined ICE analysis of...contact us at [email protected] if you have any questions, and check out the Synthego blog for more great...

...attention, though in reality much of our research still fails to reach our primary target audience – colleagues... disciplines and the years. Which leads to the question of how we can support researchers as they try ...much anyone applying the scientific method to a question. Videos from any discipline are welcome. Up to...

...specific operational or commercial challenges or questions that clients face imminently. Consulting firms...research, drugs, the diseases they can treat, and diagnostic advancements. Consultants can help these teams...weight with your scientific expertise. Do I have to stick with consulting in the long-term? A few years as...

...a subpopulation of PC9 lung cancer cells had a distinct transcriptome that was associated with future ...suitable tools. To address this challenge, the Institute for Protein Innovation (IPI) is developing highly...tools will enable researchers to interrogate the distinct roles of neurexin and neuroligin family members...

...By Justin Ng and Alyssa Cecchetelli Science is a constantly evolving and demanding field, requiring a...feature genetic, physiological, or developmental characteristics that make them ideal for studying in a laboratory...regenerate, the axolotl has several attractive characteristics that make them an ideal model organism; they...

...2013). Since then, Spinach2 has been used to investigate intracellular viral DNA dynamics in neuronal ...developed into a split aptamer that can be used to investigate RNA assembly. The split aptamer is composed of... blog Learn more about aptamer fluorophores Investigate other fluorescent biosensors Find other uses ...

...this post. Adeno-associated viruses (AAVs) make fantastic gene delivery vehicles for episomal gene expression...delivered through transfection of naked DNA. Other investigators who had been working to develop high efficiency...Central PMCID: PMC5568673. 8. Howes, Rob, and Christine Schofield. "Genome engineering using adeno-associated...

...urotensin. The MTRIA sensors will be useful for investigating neuronal signaling and for engineering improved...better response kinetics and photochemical characteristics than previous NIR calcium reporters iGECI and... to the potassium concentrations in cells than existing sensors. Using genome mining and structure-guided...

...focused scanning libraries. PFunkel: Of prior existing comprehensive mutagenesis methods, PFunkel offers...). If so, the nicking mutagenesis protocol will still work if the orientation of the BbvCI site(s) of .... PubMed PMID: 20676978. 3. Wrenbeck, Emily E., Justin R. Klesmith, James A. Stapleton, Adebola Adeniran...

...quantification). But what if you’ve got several interesting protein targets and you want to know if they ...fluorophores, then labeling with the other, you can still record the signal separately for each target.   ...your next multiplex IF experiment! If you have questions or any favorite tips and tricks, leave a comment...

... unbound by protein. These unbound regions are “sticky” and have the potential to bind nonspecifically...milk, bovine serum albumin or other proteins. The sticky areas on the membrane will bind to the proteins... thoroughly. Vendors will frequently include suggestions for working concentrations, blocking buffers,...

...non-homologous sequence or a series of single-nucleotide substitutions along a 1.5 kb tract, all without selectable...found that mating improved editing efficiency suggesting that sperm are more permissive to Cas9 editing...CRISPR plasmids page. Future perspectives It is interesting to note that recipients for the 2015 Breakthrough...

...tool for predicting CGBE editing outcomes and assisting sgRNA selection is available at http://www.sunlab.fun...CGBEs. What's next? Base transversion editing is still advancing rapidly. If it follows a similar path ... synthesized, templated sequence. And the story still isn’t over! Keep an eye out for our next post about...

... to test the selectivity of your antibody. But testing is not one size fits all. The approaches used will...found that it is often best to determine this by testing them. For intracellular targets, we usually test.... The specific approach will depend on the characteristics of the target and whether suitable cell types...

...-019-0193-0 Walton, R. T., Christie, K. A., Whittaker, M. N., & Kleinstiver, B. P. (2020). Unconstrained...variants to develop five new base editors with distinct PAM sequences, expanding the number of available...

...concepts. Each short video answers an Antibodies 101 question, like "what is an antibody?" or "what is an IgE...scicomm ♬ original sound - Addgene We'll be posting these animations regularly from now until May, ...

...decorating your lab for the upcoming wintertime festivities! Help us spread some cheer by snapping a photo...Christmas, Hanukkah, ugly sweaters, Flannel Fridays, Festivus— you name it, we want to see it.      All photos...

...upcoming wintertime festivities: Hanukkah, Christmas, ugly sweaters, Flannel Fridays, Festivus— you name it,...

...Experiment.” Whatever your antibody application question, the Antibody 101 eBook is a great place to start...reach out to [email protected] with any thoughts, questions, or feedback. Happy reading! ...

... continue to grow the ways we share science by hosting our first ever CRISPR Week on social media. #AddgeneCRISPRweek...like to see what you think so we’ll ask you some questions on Friday - share with us how you use CRISPR. ...

...ship to, first on a temporary and now on a more lasting basis.   Table 1: Popular Rabies and VSV-G Plasmids...license fee, will be required. If you have any questions on ordering plasmids, please feel free to email...

...for your TikTok or Instagram story, we also have stickers to help express your emotions (while you’re on...when you use your favorite one. If you have any suggestions for future GIFs, feel free to let us know on ...

...Dear scientists using AAV, We hear your questions! How much virus should I inject? How long do I need... patterns, and more. If you have feedback or suggestions about the AAV Data Hub, we welcome you to share...

...the details. Chapter 3 - Answering interesting biological questions using microscopy. Chpater 4 - Research...

...genus members to the specified international institution. Requesters will need to provide a brief description...Bureau of Industry and Security. If you have any questions about this or other Addgene shipping and ordering...

...antibody deposit (the Integrin collection) from the Institute for Protein Innovation. An Integrin Antibody ...Flow Cytometry Rob Hurt, whose expertise and suggestions were critical to the development and writing ...

... pPSU1 and pPSU2. When restriction digested with PstI or EcoRV, these plasmids generate 100 bp or 1 kb...prep kit increases the costs to $20-30 per ladder: still significantly cheaper than commercial ladders. Are...

...lab manager, research technician, or Principal Investigator. How-to guides for troubleshooting your experiments...involved. So join the conversation! Do you have a question about culturing iPS cells? Confused by which system...

...re following all safety regulations. That’s one sticky situation our customer service can’t get you out...our lab in Bayliss Building @SMS_UWA is getting festive 🎄🎁🎅🤶🎄 pic.twitter.com/rDaEjxTNZX— Heng Chooi...

... in Ghana. He’s done research at a number of institutions in Ghana and most recently got his PhD in cell...back to Ghana after his PhD and the need to de-mystify molecular biology. 25:51 - 30:56 : Further biology...

...students can sometimes be elusive or have a certain mystique that makes them difficult to approach for the ...to join? 11:25 - 11:50 : What is the number one question you think rotation students should ask senior ...

...peek in case you missed them. July 2016 was a fantastic month for the Addgene blog. We achieved roughly...to comment on any of our posts if you have any questions or useful insights. If you're interested in blogging...

...Addgene is uniquely positioned to help research institutions maximize their increasingly limited resources...investment isn't lost. Universities and research institutions can turn to Addgene as a trusted partner for...

...bottle instead of buying yourself a big stack of plastic cups. By initiating little changes in ourselves...could be: Setting up a recycling program at your institute. Set your -80 °C freezer to -70 °C and teach other...

... He returned to academic research in 2005 to investigate synthetic biology at Boston University, working...yeast synthetic genome project. His team also investigates bacteria and extremophiles for biotechnology...

...While we were successful in creating SynORI, an interesting observation was that their stability in the absence...negative feedback regulation from ColE1 family, it still contains a truncated RNA primer that may interact...

...new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools... was developed by Rebecca Shapiro’s lab. After testing dCas9 fusions with transcriptional repressors, ...

...lab at the Broad Institute and was designed and cloned by Neville Sanjana. This fantastic research tool ...