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We narrowed to 838 results for: ANG

Showing: 381 - 400 of 838 results

  1. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ... these proteins will be tested. Investigating changes in neuronal activity after ischemic brain injury... Tsakanova to image cortical neurons and study changes in neural activity after ischemic brain injury.... diverse groups of scientists working in a wide range of research fields and geographical locations. In...

  2. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...protein (mCyRFP1). Derived from cyan-excitable organge fluorescent protein (CyOFP1), some of the exciting...recombination. Find PhoCl plasmids at Addgene. Zhang W, et al. Nature Methods. 2017. PubMed PMID: 28288123... also provided a detailed protocol at Protocol Exchange. Plasmid maps can be found at Addgene and in Figure...flame depositor David Liu’s lab increase both the range and specificity of base editing. Kim et al. used...

  3. Hot Plasmids - May 2022

    Type
    Blog Post
    ...microbial SSAPs. Image from Wang, et al, 2022.  Find dCas9-SSAP plasmids here! Wang et al. Nat Cell Biol....target. Across all loci tested, error rates overall ranged from 0.3% to 10%, in comparison to the 62-90% error...

  4. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...heavy (blue) chains as driven by two CMV promoters (orange). Figure from Andrews, et. al., Elife  A limitation...step would be to validate your new construct by Sanger sequence analysis. But for this research, it was...express multiple subclasses of IgG antibody. Broad range secondary antibodies are commonly used in research...

  5. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...these reporters, editing typically results in a change in the fluorescent readout. In this blog we will...for easy screening of different gRNAs by simply changing the gRNA used when transfecting your cells.  A...GEmCherry2. The Denham lab made several other small changes creating 2 other versions but these did not surpass...

  7. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    ...bacteria out of their optimally suited temperature ranges and make them grow better at suboptimal ones, which... of Earth’s harshest environments: corrosive pH ranges, crushing pressures, near-melting temperatures,...discussions. Not only are such endeavors a welcome changeover from conventional academic settings, these are...

  8. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...articulating why the results you've measured should change established theory. So, what can you do to ensure...you receive generalizes.  If someone suggests a change to a slide, think about whether the same principle...explain why the work is important and how it should change how the members of your audience think or act.   ...

  9. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...CRISPR-Cpf1 genome editing!  A recent paper from Feng Zhang's lab describes how to use Cpf1 for multiplex genome...tracrRNA? Yes No dsDNA cleavage Blunt end 5' overhang PAM sequence NGG TTTV PAM site preserved? ...Slaymaker, I., Makarova, K., Essletzbichler, P.,  Zhang, F. (2015). Cpf1 Is a Single RNA-Guided Endonuclease...

  10. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...the VSV-G envelope provides the widest tropism or range of cells a virus can infect. Note: While both lentiviruses...and γ-retroviral packaging plasmids are not interchangeable. General envelope plasmids, such as VSV-G (... vice versa. Envelope plasmids may be used interchangeably. Are γ-retroviruses replication incompetent...

  12. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...overlaps with the XbaI cut site (depicted as an orange line) because the restriction site is followed ...mutated plasmids which can later be verified by Sanger sequencing. How can I tell if my enzyme will cut...will be blocked by methylation and how that may change your expected digest results. If Dam methylation...

  13. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...upon themselves to teach me Python, a programming language favored by biologists. Almost as soon as I began...like a wet lab experiment I use the text editor TextWrangler to record the following, which are later printed...that further helps you keep track of your exact changes. Alternatively, you can simply make a note in your...

  14. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...paper, Yinong Yang’s group did just that as part of their CRISPR work in rice plants. Yang was intrigued...endogenous tRNA-processing system. Xie K, Minkenberg B, Yang Y. Proc Natl Acad Sci U S A. 2015 Mar 17;112(11)...

  15. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ....  Here are some tips to get out of the rut of hanging out with only your lab mates (let’s face it, you... can also see and offer congratulations on job changes and promotions.  Follow academic scientists on ...just a meeting, but having lunch twice turns a stranger into a friendly colleague. Recommended Reading...

  16. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    ...s Lab in 2009. From 2009 to today, Sample6 has changed focus and names, swapped leadership, and grown ...much learning, the focus of the application was changed, but Sample6 still leverages the core engineering...better than current technology and get customers to change behavior. When you are ready to commit to the company...

  17. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...nutrient and ionic balance of the cells,” says Dr Sangeetha Iyer, ACD’s Scientific Advisor. It’s possible ...Dr. Dianna Milewicz, Dr. Carolyn Bondy, and Dr. Angela Lin got me interested in TS when I was a newly ... Jennifer Tsang...

  18. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...split base editors Article contributed by Jennifer Tsang AAVs are a great gene therapy delivery mechanism...are proteins that detect and output the voltage changes in neural activity when paired with appropriate...optogenetic actuators.  To overcome this overlap, Yiyang Gong’s lab created two plasmids containing a red-shifted...

  20. Antibodies 101: Conjugation

    Type
    Blog Post
    ...microscopy, flow cytometry, or spectrophotometry. The range of fluorophores available for antibody conjugation...and ELISAs. Chromogenic reactions produce a color change, so they are typically used in microscopy-based... choice for ELISAs.  Biotin Biotin is used in a range of antibody-based assays. Biotin itself is not visible...
Showing: 381 - 400 of 838 results