We narrowed to 492 results for: ARA-2

...protocols 4.4 (2009): 495-505. PubMed PMID: 19300443. 2. Geraerts, Martine, et al. "Comparison of lentiviral...if preparing several different lentiviruses in parallel, this approach should be considered. Similar to...consider using a reverse transcriptase mutant in a separate control reaction. This will allow you to quantify...

... adapted to a competitive format. Figure 2: Overview of using Competitive ELISA to quantitatively... wells with a purified protein allows you to characterize properties of an antibody that recognizes that.... As mentioned above, it can also be used to characterize antibodies against the antigen bound to the ...

...more tips on using social media as a scientist here. 2) Public engagement - Participate in science festivals...book.  Many thanks to our guest blogger, Sarah Schmidt! Sarah Schmidt is engineering resistance against...This post was contributed by guest blogger Sarah Schmidt, a Marie Curie Fellow at The Sainsbury Laboratory...

...products have distinct localizations. Figure 2: Split-wrmScarlet labeling proteins with different... than split-sfCherry3. It was created by Maria Ingaramo, Jerome Goudeau, Cynthia Kenyon, and colleagues...Cas9-Cas12a gRNAs under a single U6 promoter. The paralog and dual-targeting hybrid gRNA library can be used... used to identify genetic interactions between paralogs. The exon-deletion hybrid gRNA library deletes...

... Cure for HIV/AIDS. Int J Mol Sci. 2023 Jan 13;24(2):1563. doi: 10.3390/ijms24021563 Resources on Addgene.org... therapeutic potential in mice with a dominant cataract disorder caused by a single copy of a gene known...known as Crygc (Wu et al). They found that the cataracts phenotype could be rescued by zygote co-injection...polymorphisms (SNPs) in the TTR gene and is molecularly characterized by misfolding of the TTR protein, which contributes...

... to remove this default setting. Timesaving Tip #2: blastx searches are inherently slower than blastn... BLAST button, consider the Program Selection parameter, as this will affect the amount of time to perform...BLAST button, you may have noticed the “Algorithm parameters” link. Click on this link to view advanced BLAST...have to remember to adjust any blastp algorithm parameters. We use the default scoring matrix BLOSUM62, ...

... those that also infect the liver.    Fig. 2: Summary of the generational evolution of the AAV ...SSAP system reports an efficiency of up to 20%, comparable to commonly used CRISPR/Cas9 methods for large...

...Era of Personalized Medicine. Genes Dis. 2017 Jun;4(2):43-63. DOI: 10.1016/j.gendis.2017.04.001 Maetzig.... 2011 Jun;3(6):677-713. DOI: 10.3390/v3060677 Varanda C, Félix MDR, Campos MD, Materatski P. An Overview...

...PubMed PMID: 8491198. PubMed Central PMCID: PMC413431. 2. Bukrinsky MI, et al. “Active nuclear import of human...We will be covering these safety measures in a separate, upcoming post. If a viral vector seems like ...

... high-priced commercial devices.     Fig. 2: Workflow for open software (left) vs. open hardware...in developing better, more sustainable, easily sharable products. Open-Source Microscopy Sharing Hardware...

... scientific communication ecosystem Figure 2: The scientific ecosystem, enriched by open access...bioRxiv. https://doi.org/10.1101/2021.09.07.459259 Sarabipour S, Debat HJ, Emmott E, Burgess SJ, Schwessinger...

...of human codon-optimized  cryptochrome 2 (CRY2) from Arabidopsis thaliana. When exposed to blue light, ...protein. After fusing the CRY2 and CIB1 domains to separate proteins of interest, a researcher can cause them...

...dimerization-dependent protein interactions. FEBS letters, 591 2, 415-424. PubMed PMID: 28032891. This is a different...feature is particularly useful for in-depth characterization of dynamic protein complexes. Split-BioID,...of the bait proteins’ interactome. Further characterization of these candidates is critical to understanding...

... already have an end product in mind, steps 1 and 2 will generally be straightforward. Likewise for step...present in a single lentiviral transfer vector or separate transfer vectors. May contain reporter gene (e.g...model.  If your expression system is not well characterized in terms of CRISPR use, you will want to invest...

...GUS-staining is a very useful visual reporter (Figure 2). The GUS enzyme cleaves a substrate, which will then...vincristine, are regulated in the medicinal plant, the Catharanthus roseus (Figure 1). The ultimate goal of our ...   C. roseus is not a model organism such as Arabidopsis or tobacco. Studying gene function in C. roseus...method for overexpressing genes In plants like Arabidopsis and tobacco, Agrobacterium infiltration into ...expression assay by agroinfiltration of leaves." Arabidopsis Protocols. Humana Press, 2006. 225-229. PubMed...transient gene expression analysis in seedlings of Arabidopsis and other plant species." Plant methods 5.1 (...expression method for analyzing gene function in Catharanthus roseus seedlings." Frontiers in plant science...

...system (by measuring the fluorescence intensity); and 2) to visualize the localization of the FP (fused to...experimental parameters (e.g. excitation light intensity, pH, or temperature). pH Stability: This parameter is ...Photostability can be affected by experimental parameters (e.g. excitation light intensity, pH or temperature...

...the necessary steps to perform:      Fig. 2: Quick-stepping to a FASTA file   With our outline...QC'ing data at Addgene is like standing under a Niagara Falls sized rainbow   Over the years we have...pipelines, including bioinformatics. We loved the parallels between Seqera’s mission and our own, as both ...the sequential tasks in our pipeline, we wrote a separate Nextflow process. In Nextflow, a process defines...concept of channels and implemented the input declaration correctly, the script block in the second process...

...inside the cell,” says Pedre Pérez. Figure 2: Micrographs of the biosensor expressed in the human...colleagues Filip Tomaska and Tim Hanson, are characterizing variants of iGluSnFR3 in vivo and have used...

...overview of these new variants:   Figure 2: Inner mitochondrial membranes imaged with structured...and screening that led to identification and characterization of AAV.CAP-Mac. Image reproduced from Chuapoco... Endothelial-specific AAV delivery By Cythina Arokiaraj (Gradinaru lab) Another useful viral tool deposited...

...You’ve probably heard that only 2% of our genome is made of protein-coding genes, and you might be wondering...transcriptome engineering References Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E. A Programmable...CRISPR-Based Approaches for the High-Throughput Characterization of Long Non-Coding RNAs. Non-Coding RNA. 2021...