...topics with the researchers who joined a recent Twitter chat for Early Career Researchers.  Many of the ...podcast interview with Harvard Medical School researcher Connie Cepko to learn about her mentoring style...success. There were unfortunate stories from researchers with advisors who did not serve as good mentors...essay Acclimatrix, an assistant professor at a research university in the northeast, writes a thoughtful...

...series will help you find new CRISPR tools for your research! This time: GeneWeld vectors to create knock-...inhibition library CRISPR knockout libraries for cancer research Creating knock-ins with GeneWeld vectors GeneWeld... and editing. Read the paper in Nucleic Acids Research Find the drug inducible Cas9 plasmids E. coli... Library CRISPR knockout libraries for cancer research The Sidi Chen lab recently published two CRISPR...CRISPR knockout libraries for cancer research. The Mouse Metastasis CRISPR-Cpf1(Cas12a) Double Knockout Library...

...encourage researchers all around the world by showing them that they are not alone; a researcher doing some... people all around the world to the lives of researchers and PhD students. Everybody in science knows ... experience this very human side to scientific research. Find Protocols Lab Tips to Keep Your Experiments...Blog Learn How You Can Use Video to Share Your Research 5 Reasons to Use Reddit for Science Communication...

...presented my research to Addgenies and friends. These invited guests came to see the research from all the... interview process with Amy, who let me know in March that I successfully secured an internship at Addgene...project was based on plasmid cloning and I, the researcher, got to choose what gene to focus on. The gene...workspace — and got the chance to work on my very own research project! Coming from an environment with limited...

...plasmids and your research stories. Consider practicing communicating your research by guest blogging ...make it a habit to talk – really talk – about the research you are doing in the lab with your mother or father...for science outreach. Here in the North Carolina Research Triangle area, scientists give talks and science...goal is ostensibly to get some funding for your research, SciFund is really motivated more by the desire...

...that will teach you how to: interpret data, solve research problems, develop general technical proficiency...professionally with other scientists. The specific research area is not as important as choosing a supportive... want to work on in your future career, so the research topic will be more important. In addition, it ...podcast interview with Harvard Medical School researcher Connie Cepko to learn about her mentoring style...use publication records, university websites, ResearchGate and LinkedIn to find past lab members. Most ...

...own and only share what they think will get the research funded,” she says. Baradaran met Addgene Executive...reagents such as plasmids and viral stocks for researchers around the world who would otherwise have difficulty...Discovery at the Novartis Institutes for Biomedical Research. Drug development was something Stegmeier has ... Novartis, Stegmeier deposited the ClonTracer Barcoding Library, a library that “enables the high-resolution...got incredibly compelled by wet bench molecular research, but also became passionate about virology,” he...

...with whoever cares to listen. In March 2020 she will start her own research group at Boyce Tompson Institute...contributed by Magdalena Julkowska, a postdoctoral researcher at KAUST, Saudi Arabia. From the perspective...to the subsequent journal.  As a postdoctoral researcher studying plant development and abiotic stress...salt-stress induced responses in Root System Architecture of Arabidopsis HapMap population in Amsterdam...

...contributed by Richi Sakaguchi from Kyoto University, and Marcus N. Leiwe and Takeshi Imai from Kyushu University...regularly and hope Tetbow can help you in your research.     To see Tetbow in action, watch the video...bloggers Richi Sakaguchi from Kyoto University, and Marcus N. Leiwe and Takeshi Imai from Kyushu University...genetic tools for neuronal circuit tracing.     Marcus Leiwe is an assistant professor at Kyushu University...PubMed Central PMCID: PMC4246963. Sakaguchi, Richi, Marcus N. Leiwe, and Takeshi Imai. "Bright multicolor ...

...over and over again until you don’t.” As every researcher can attest to, science is nothing if not solving...I sit down and talk to a scientist about their research, these conflicts come out quite naturally as they...What did those odd results mean? How did the researchers solve the problem preventing them from moving...questions are next or how this will impact future research or policy.  It is worth mentioning that this is...audience a chance to get curious and invested in the research itself - and isn’t that sense of curiosity and...

...was contributed by Marco Straccia, an Associate Professor at University of Barcelona. The challenge: Making...fre-sci.info Marco Straccia is currently an Associate Professor at University of Barcelona. He is also ..., I and other professors at the University of Barcelona wanted to develop strategies to make our classes...

...experiments and other researchers by implementing reproducibility best practices in your research. For a place ...GHP_HarvardChan — Shekhar Saxena (@ssaxenageneva) March 16, 2020 Give back to your community. For those...experiments.  Use Addgene’s AAV Data Hub. You can search the AAV Data Hub for tools to use in future experiments...contribute data to the AAV Data Hub to help the research community. Set expectations for your productivity...

...meaning, making it difficult or impossible for researchers to track down that resource. A more long-term...information, and more. The plasmid page also helps researchers find other publications that have used the same...support standardized persistent identification of research reagents, several journals or organizations now...several journals like eLife, Nature, and Cell use  Research Resource identifiers (RRIDs) which are supported...repository are verified and identifiable to save researchers time and make their work more reproducible. While...

...Loren Looger’s lab at HHMI Janelia Research Campus to give researchers the ability to monitor glutamate ...iGluSnFR3 To improve upon the SF-iGluSnFR, the researchers did the following: Improve sensor’s photophysical.... In addition to the original PDGFR TMD, the researchers introduce iGluSnFR3 in two additional anchoring...Stargazin including a terminal PDZ ligand. The researchers recommend using the SGZ for in-culture work only...

... S. cerevisiae and S. pombe have dominated the research scene. But what about the other yeasts – in the...all, but it’s not as well-known in the general research community. Phillips Petroleum Company first commercially...the K. phaffii expression system to the general research community in the 1990s when they abandoned the... and pharmaceutical companies (along with some research laboratories) have picked up the system for some...phaffi as Emerging Model Organisms in Fundamental Research. Front. Microbiol. 11-2020, 2021. DOI: 10.3389...

...including over 3,500 human TF isoforms, to help researchers understand this regulatory network. The transcription...library of each unique TF open reading frame (ORF), barcoded for tractability. These ORFs are individually ...generated (Joung et al., 2023). Fig. 1: Barcoded TF library was packaged in lentiviral particles... and screening mechanisms, although the unique barcodes for each TF help streamline identification. While... test prophylactics on and a program that any researcher could use to generate the relevant disease model...

...guest blogger Chris Richardson, a Postdoctoral Researcher in Jacob Corn’s lab. CRISPR-Cas9 (Cas9) is an...strategies to manipulate that process. We began our research with a simple question: how do Cas9 and sgRNA ...donor DNA. Chris Richardson is a Postdoctoral Researcher in Jacob Corn’s lab investigating DNA repair ...delivery of purified Cas9 ribonucleoproteins." Genome research 24.6 (2014): 1012-1019. PubMed PMID: 24696461....Our CRISPR Guide Find CRISPR Plasmids for Your Research Check out Our List of gRNA Design Tools ...

...requirements. These different PAM requirements allow researchers to target many different genomic loci. 3. Cleave.... When using CRISPR/Cas9 for genome editing, researchers simply need to express a gRNA designed to direct...elements separated by unique sequences. When researchers first discovered these arrays, they did not know...them “spacers” (see figure below). After years of research, we now know that each direct repeat, combined...leaving a F) double-strand break. A “gRNA” is a researcher-designed hybrid of the tracrRNA and the crRNA...

...story of base transversion editors starts with researchers in the Keith Joung lab, who were engineering ...editing between bacteria and mammalian cells Researchers in the Xueli Zhang lab were making similar observations...develop more efficient and precise editors. Most researchers agreed on the first part of the likely editing... efficacy varied among target sequences. The researchers hypothesized that the CGBE edit process occurs...experimented with adding different proteins to the CGBE architecture. They conducted a CRISPRi screen to identify....1038/nbt.4172 Kurt, I. C., Zhou, R., Iyer, S., Garcia, S. P., Miller, B. R., Langner, L. M., Grünewald...

...continue to accelerate research and discovery by improving access to useful research materials and information...have been lost to continue to be available to the research community.    Validation For most cloning projects...construct by Sanger sequence analysis. But for this research, it was likely that the sequence of the functional...range secondary antibodies are commonly used in research laboratories to detect the different subclasses...