... your edit, it’s important to know a few things about your target cells and the desired edits. First, ...et al., NAR). It works just like TIDE (outlined above), except that it uses a 3rd sequencing trace file...great option, especially if the options outlined above aren’t realistic. Check Out Our Post on Sequencing...higher probability locations. If you’re worried about off-target effects, you can consider using a version...SpCas9-HF1, eSpCas9(1.1), or HypaCas9. Read more about Cas9 options in our blog post here. Dealing with...

...I spent about seven years working in research labs, and then pivoted to writing full-time in mid-2020...Synthetic Biology One of the best ways to learn about a new field is simply to know where to look and ...might be helpful, please reach out to me by email.  About the author Niko McCarty is the editor of Asimov ...curriculum at MIT. He also writes Codon, a blog about biotechnology.    ...

...Blog Learn more about FRET Tips for Using FRET in Your Experiments Learn more about Fluorescent Protein...heme-containing proteins in whole animals, light at or above 600 nm wavelengths should be used. Researchers often... emits light at a tissue-penetrating wavelength above 600 nm. LumiFluors Schaub, F.X., et al. (3) designed...be excited by NanoLuc® luciferase (NLuc) and set about to create an in vivo imaging reporter by linking...

...subjected to the experimental condition. In the example above, a negative control could be a third group of mice...in the experimental protocol. In the experiment above, you could repeat the tau western blot several times...Baseline secondary signaling levels In addition to the above-mentioned safeguards, you’ll need to test the secondary...Flow cytometry In addition to the controls listed above, viability and compensation controls are recommended...technique before starting. For more information about antibodies and antibody-based experiments, check...

...can be used in the repressible manner described above, as well as the inducible manner detailed in our...necessary to have all 3 of the different binary systems above if they all behave very similarly?” The simple answer... at the same time.  The 3 binary systems listed above are orthogonal, meaning that they do not affect ...Srikrishnan. “Introduction and expression of genes for metabolic engineering applications in Saccharomyces cerevisiae...

...Science with Tom. In this podcast, we learn more about Tom and pick his brain for advice on how to dive... Rap of Henrietta Lacks Students in Oakland rap about Henrietta Lacks - the African American woman whose...Please Don't Kill My Hive Beautifully filmed video about colony collapse disorder.     5. Plasmid Rap ...science standards and communicate science. Learn more about Tom on his youtube channel and at sciencewithtom.com...

...this new way to learn about plasmid technologies. If you have any comments about the Hot Plasmids Podcast...with these efforts, you’ll find new ways to learn about science careers, lab protocols, and, of course, ...each one, plug in some headphones to learn more about the latest and greatest plasmids through this podcast...

...therapeutics at Novartis - listen to learn more about Crystal’s exciting career path, to gain insight ...Chapter 2: What's a lab group in industry? The collaborative nature of working in industry and how delegating...metrics for success. The importance of thinking about the big picture and not just the next experiment...resources in our Mentoring for Scientists Series Learn about Turning Your PhD into a Career with Joanne Kamens...

...Plasmids 101 blog series Learn about the different grades of DNA  Read about the various ways to quantify...scientific communities using plasmid tools. Details about the cloning grade DNA beta project We will ship ...quantify DNA Resources on Addgene.org Learn about our other beta projects Read our Molecular Biology Reference...

... Its Fruit Is Sweet The Future? I knew little about adeno-associated Viral Vectors (AAVs) before starting...I’ll provide some tips that will help you think about how to get started with AAVs. Since my research ...understanding of receptor physiology and the effects of metabotropic and ionotropic influences on overall cell function... the biotechnology firm Biogen have recently collaborated to develop “AAV 3.0”, vectors that will have...serotonin in movement. Follow her on twitter @katrinaboards.   References 1. Russell, David W. "AAV vectors...

...Find more blog posts about CRISPR Learn more about the history of CRISPR Read about other genome editing...Science Fiction (SciFi) writers have been curious about the capability of transforming the genetic code ... of California San Diego. She is working in collaboration with Prof. Alexis Komor and together they are...

...How did you hear about post grad opportunities and when did you start thinking about them? 18:30 - 19:... top if you leverage your community, think hard about picking a good mentor, and begin considering career...19:54 : How to think about picking a post-doc project 19:55 - 22:40 : Improvements that can be made to ...

...blog Learn about recycling initiatives for the lab Subscribe to the Addgene blog Learn about other creative.... In addition to doing some of the quick things above, try joining or starting a sustainability project...campaign! MIT Green Labs has a lot of resources about this, and even has a list of biological samples ...

...learn about all the amazing research being done with plasmids. I heard an impressive talk about the use...pulls off such an inspiring, thought-provoking, collaborative event – all while keeping the party going! At...scientists we've spoken with are excited and passionate about making such tools open-access. Though it is time...

... questions about the ordering process, the Addgene Help Center is full of information about how to deposit...requires a quote in order to generate a PO, learn more about how to create a quote. How do I pay the invoice...Addgene Help Center If you have additional questions about our ordering process not addressed in this article...

...updated AAV Data Hub to share curated information about AAV usage in research applications. Scientists around...world, are learning to navigate the changes brought about by the pandemic. As we adjust, we are reflecting...I have spent the last 9 months quietly learning about Addgene’s past and present, but the real promise...forward to working with Addgene colleagues, collaborators, partners, and the larger scientific community...

... highlighted reagent repositories and other collaborative efforts in the scientific community to speed...store my plasmid in TE, Tris, or water? And what about temperature? Find the details in this blog post....dominates DNA quantification discussions, but what about the other methods to quantify DNA? Here we cover...Incompatibility by Leah Schiesow If you're thinking about hosting multiple plasmids in one cell, it might ...

... to design gRNAs for all the purposes discussed above. Please see videos and text on the website for more...Output: A text file containing full information about the suggested gRNAs to use, including all information... hard to read. It´s suggested that you open the above text files, copy the content of the file, and paste...Additional Resources on the Addgene Blog Learn about CRISPR Screens How to Design Your gRNA Tips for ...CRISPR User Resources on Addgene.org Learn More About CRISPR Find CRISPR Screening Libraries Browse All...

...ChIP-seq methods provide a wealth of information about how protein networks work in cells. A major bottleneck...two-part cr/tracrRNAs for CRISPR experiments (in collaboration with Integrated DNA Technologies, USA). These...commercial suppliers without investing time and labour on reagent preparation in the lab. Figure 3: csRNP-assisted...first crRNA we designed for ~250 genes, we have had about 30% tagging success, although many of these genes... When not pipetting, he can be seen daydreaming about sunny days in the UK.   References 1. Bressan RB...

... constructs that compose genetic circuits and metabolic pathways. For many MoClo systems, the individual...interested in recombinant protein purification and metabolic engineering. If instead you want to build genetic...designed for flexibility. The Fragmid kit described above works in both mammalian systems and Drosophila. ... Signaling in CAR T Cells. Cold Spring Harbor Laboratory, 17 Feb. 2024. Crossref, https://doi.org/10.1101...Emerging CRISPR Technologies. Cold Spring Harbor Laboratory, 27 Oct. 2023. Crossref, https://doi.org/10.1016...