Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 710 results for: BASE;

Showing: 381 - 400 of 710 results

  1. Bacterial Expression Systems

    Type
    Collection
    ... protein–protein interaction. The cpFRET - FRET-based Biosensors Kit (Addgene #1000000021) is a vector...Addgene Blog CRISPR Methods for Bacteria CRISPR-based Genome Editing Systems For Editing in E. coli INTEGRATE...

  3. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...is vital for the accurate interpretation of FRET-based experiments. A popular combination is Alexa594, ...dyes, expanding the capabilities of fluorescence-based techniques in biotechnology. Recent examples of ...2016). Development of a quantitative fluorescence-based ligand-binding assay. Scientific Reports, 6. https...

  4. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...event, and assigns each event to a channel number based on intensity. Higher intensity events are assigned...to the protein in its native state.  Antibodies-based flow cytometry can use both direct and indirect ... background information for setting up antibody based flow cytometry experiments.       References and...

  5. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...questions, which are critical in setting up a FRET-based assay or screen (the Addgene blog has an excellent...different city, you can still develop a successful FRET-based assay since energy transfer only occurs when donor...10. Saraheimo, Satu, et al. "Time-Resolved FRET-Based Approach for Antibody Detection–A New Serodiagnostic...

  6. Pooled Library Amplifications

    Type
    Blog Post
    ... electroporations. The specifics of this can be based on the manufacturer’s protocol for transformation...transformation efficiency, or more specifically calculated based on the size of and weight of your library plasmids...

  8. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...point where the hydrogen bonds break between the base pairs. This results in the separation of the two...using PCR. qPCR requires the addition of a probe based fluorescent dye that intercalates with any dsDNA...

  9. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ... scanning By Seth Kasowitz As multiple antibody-based vaccines are deployed around the world to combat...deactivate photocleavable gRNAs within seconds. A CRISPR-based lineage tracing tool captures rare clones that represent...

  11. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    ... John Doench et al. shows that mutating a single base pair may not be enough to prevent re-binding. Lastly...region in a UTR, CrispyCrunch flips the two important base pairs of the PAM (NGG -> NCC, CCN -> GGN). This ... much as possible, it uses standard tools and databases, including primer3, biopython, bwa, samtools, ...

  12. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...quickly and inexpensively generate a potent Cas9-based transcriptional regulator without making any functional...date his group has develop several popular CRISPR-based tools for altering DNA sequence or regulating gene...

  14. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ... question to ask is how the efficiency of PITCh-based genomic insertion would compare to CRISPR sticky-end...Mathieu, et al. (2015). “A Scalable Genome-Editing Based Approach for Mapping Multiprotein Complexes in Human...

  15. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...and fluorescence. Biosensors: A wide array of GFP-based fluorescent biosensors has been designed to detect...separates mixtures of cells into distinct populations based on fluorescent signal. Thus, FACS can be used to...

  18. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...consistent development and improvement of CRISPR-based technologies over the past decade. CRISPR screens... fact supported by the wide array of lentiviral-based CRISPR libraries available at Addgene. The large...

  19. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...extrapolate the concentration of the unknown sample based on its measured response. An example of this is ..., the more accurate the curve. Though it varies based on the assay, the ideal %CV is less than 15%. You...

  20. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...Addgene blog Introduction to Antibodies Plasmid-based Recombinant Antibodies Polyclonal Antibodies Resources... Shokri A, Chotteau V. Antibody capture process based on magnetic beads from very high cell density suspension...
Showing: 381 - 400 of 710 results