... different categories based on function: knockout (KO), activate, inhibit, base edit, and other. The first...

...point where the hydrogen bonds break between the base pairs. This results in the separation of the two...using PCR. qPCR requires the addition of a probe based fluorescent dye that intercalates with any dsDNA...

... scanning By Seth Kasowitz As multiple antibody-based vaccines are deployed around the world to combat...deactivate photocleavable gRNAs within seconds. A CRISPR-based lineage tracing tool captures rare clones that represent...

...available filtering options now change dynamically based on the type of material and specific search terms...curated pages categorized by both functionally (e.g. Base Editing) and by target organism. Clicking through...

... question to ask is how the efficiency of PITCh-based genomic insertion would compare to CRISPR sticky-end...Mathieu, et al. (2015). “A Scalable Genome-Editing Based Approach for Mapping Multiprotein Complexes in Human...

...and fluorescence. Biosensors: A wide array of GFP-based fluorescent biosensors has been designed to detect...separates mixtures of cells into distinct populations based on fluorescent signal. Thus, FACS can be used to...

...Novartis, a multinational pharmaceutical company based in Switzerland. After completing her PhD in Chemistry...

...antibodies in Addgene’s repository  IBEX Knowledge-Base  Any IBEX multiplex tissue imaging Data repository...

...community. The Penn Vector Core will produce viral-based vectors for distribution via Addgene. For a full...look up a viral vector with the Penn catalog ID: Based on low demand, production of some viral vectors ...still available, other serotypes may be available) Based on low demand, production of some viral vectors ...

...TEV cleavable 6x-His tag Ophear Gileadi pET-28-a based expression vector containing N-terminal, TEV cleavable...Anhydrotetracycline, lactose, IPTG Richard Murray Plasmids based off of pBESTluc from Promega. Contain a variety ...

...less photostable than GFP. In addition, antibody-based amplification of their signals is weak and increases...specific antibody binding sites and primary antibody-based detection, smFPs create much brighter signals for...

...different ideas. We are a huge fan of the Golden Gate based MoClo system. The vectors mentioned in this blog..."The FAST technique: a simplified Agrobacterium-based transformation method for transient gene expression...

...money. They also usually have a good event-planning base and will know how to reserve space, time events,...I think many of the students learned something. Based on the initial guidelines I received from the teachers...

...allowing for in vivo biotinylation through a plasmid-based biotin ligase. Biotinylated dCas9, with bound DNA...genomic region is another example of the many tools based on targeting of a genomic locus through a specific...

...arises here. Notwithstanding access restrictions based on permissions, however, in some cases it would ...provides the RSpace electronic lab notebook. Rory is based in Cambridge, Massachusetts, and feels privileged...

...additionally allow for visual selection of transformants based on expression of RFP in the seed (Emami, Yee and...inflorescence (the flowering portion of the plant) near the base of the stem. This buys you a few days until new ...

...demand model with weekend and holiday effects. Based on these observations, we considered the following...enable next-generation therapeutics. Prime editing, base editing, PACE, and DNA-templated synthesis are four...

...extrapolate the concentration of the unknown sample based on its measured response. An example of this is ..., the more accurate the curve. Though it varies based on the assay, the ideal %CV is less than 15%. You...

...Addgene blog Introduction to Antibodies Plasmid-based Recombinant Antibodies Polyclonal Antibodies Resources... Shokri A, Chotteau V. Antibody capture process based on magnetic beads from very high cell density suspension...

... Other fields, often highly technical or skills-based ones, may not use them very often. Similarly, entry-level...or sharing relocation plans. They can be skills-based: if you're switching fields, you can use your cover...