... a programmable epigenetic editor consisting of dCas9 fused to a DNA methyltransferase — that can drive...DNA-targeting proteins, so the team created versions using dCas9, zinc finger proteins, and transcription activator-like...miniCRISPR” version, are much smaller proteins than SpyCas9, allowing them to be packaged in AAV vectors, potentially...histone tail (H3K4me0) to a DNA-binding protein (dCas9 shown here). D3L recruits the endogenous, autoinhibited...

...for advice before running your analysis. In this case, I and other lab members had worked with lymphocytes... the positive one (Figure 8). Note that in many cases a stain will not result in clearly distinct positive...will show you a combination of two stains, in this case through conjugated antibodies targeting the cell...use multiple individual gates. Note that in this case, square gates were used.  Congratulations! ...

...translation-blocking STOP cassette immediately following the promoter. The STOP cassette includes a mutant YFP...CRISPR-Cas9 genome editing may be the hot new way to manipulate gene expression, but other gene manipulation...eliminating two color possibilities. The remaining cassette can invert as long as Cre remains expressed; once...

...Rosario F. Godino, Rocío López-Diego, & Zafira Castano Corsino. When someone says “internationalization...conceived and developed by its Director, Dr. Zafira Castano Corsino, a junior faculty member at Harvard Medical...from the International Mentorship Program Zafira Castaño Corsino is a Junior Faculty at Harvard Medical ...and mentoring activities. Follow her on twitter @zcastan or on linkedln.       Rosario Fernandez-Godino ...

...vectors fantastic options for delivering shRNA, CRISPR/Cas9 components, and fluorescent sensors. Retroviral...allowing for selection of the transduced cells. In this case your gene and the marker can be separated by an ...rtTA) to bind TRE only in presence of Dox. In this case, your gene is repressed in steady state and is expressed...directly added to your target cells. However in that case you don’t know how many viral particles you put ...

...favorite twitter #agarart accounts, Petri dish Picasso (@PetriPicasso) came together and delivered very impressive...Colorado -Anschutz Medical Campus (@CSD_CUAMC) caught a case of holiday cheer, and we loved it! Check out their...

... In this episode of the Addgene Podcast, we introduce you to the Journal of Emerging Investigators, an...interview with the Journal of Emerging Investigators Podcast breakdown Intro Chapter 1 - Introduction to Mark...the audio engineer and producer for the Addgene Podcast.      Additional Resources from the Addgene Blog...

...a peer-reviewed manuscript OR a preprint. In the case of a preprint submission, we are happy to update...cloned into it. gRNAs are used for directing CRISPR/Cas9 to a targeted genomic location, while shRNAs are...format, e-mail us at  [email protected] Special cases Please contact us at [email protected] or +1 (...) if your deposit falls into one of the special cases below:   Pooled Library A pooled library is a ...

...with his new company, Science with Tom. In this podcast, we learn more about Tom and pick his brain for...the audio engineer and producer for the Addgene Podcast.     Additional Resources on the Addgene Blog ... a Grad Student Community Check out All of Our Podcasts Find Great Educational Content in our Plasmids...

... School researcher Connie Cepko. Listen to the podcast below to hear all about the research being conducted...interview. Happy Listening! Click here to listen! Podcast breakdown: (Time : Topic) 0:00-1:00 : Introduction...the audio engineer and producer for the Addgene Podcast.     References 1.Tang, Jonathan C.Y., et al. “...

...In this episode of the Addgene Podcast, we sit down with Kwabena Duedu, a researcher at the University... to Ghanaian scientists Click here to listen! Podcast breakdown (Time : Topic) 0:00 - 7:11 : Introduction...the audio engineer and producer for the Addgene Podcast.     Additional Resources on the Addgene Blog ...

...endogenous levels (Laflamme et al., 2019). CRISPR-Cas9 approaches are commonly used to produce KO cell ...is not amenable to knockout approaches. In these cases, we would recommend a knockdown approach instead...lines based on expression of the target. In this case, you could compare the antibody’s labeling of the...Biddle, M. S., Smallwood, D. T., Weston, C. A., Castells, E., Bowman, V. W., McCarthy, J., Amrani, Y., ...

... lentiviral transfer vector used to deliver both Cas9 and your gRNA of interest to mammalian cells. It... blog post on Genome-wide Screening Using CRISPR/Cas9. If you need help cloning a gRNA into lentiCRISPR...References 1. Shalem, Ophir, et al. "Genome-scale CRISPR-Cas9 knockout screening in human cells." Science 343.6166...

... frequently used elements in plant expression cassettes: plant promoters, N-terminal tags, coding sequences...C-terminal tags, plant terminators and resistance cassettes for selection of transgenic plants. The complete... gives the option to stack several expression cassettes onto a single T-DNA in subsequent rounds, dramatically...

...have one associated with the gRNA), application, cas9 species, PubMed ID, and depositor. The CRISPR applications...nick, purify, tag, scaffold, CRISPR-display, and dCas9-FokI. This table is both sortable and searchable...alphabetically or in ascending or descending order, in the case of numeric columns.  The data in any column are ...

...Camelid family is composed of camels, llamas, and alpacas (and their undomesticated counterparts) - not exactly...soon after found similar antibodies in llamas and alpacas (Figure 1). At the time, researchers were already...started with immunizing a camelid (usually llamas or alpacas). But because sdAbs are so amenable to recombinant...

...at eScience Labs also write test question banks, case studies, and experiments for labs, in addition to...comprehensive exam proposals, a few PhD theses, and occasionally posting something science-related on Facebook...science education tools? Check out this Addgene podcast with educator and science rap mastermind Tom McFadden...

...simultaneous gene activation in plants.  FnCas12a, unlike other Cas12a nucleases identified, functions at temperatures...editing in moderate thermophiles.  These evolved SpCas9 variants have improved activity on NAG PAMS and...

...skill acquisition. Classes covered filmmaking, podcasting, philosophy of science, creative writing, designing...an animated video depicting nuclear fission; a podcast with a genetics researcher on parasitoid wasps;...the face of disparate spheres of research. In my case, an interest in neuroscience was carried over and...

...FAP - fluorogen activating protein - dL5** in this case), and 2) a fluorogen (the TAP - targeted and activated...activated photosensitizer - MG-2I in this case). The fluorogen is not fluorescent until it binds its protein...easy to watch development in real time.  In both cases, dL5** was tagged with mCerulean3 (mCer3) for visualization...