Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 716 results for: CaS;

Showing: 381 - 400 of 716 results

  1. Enabling high school research at the Journal of Emerging Investigators

    Type
    Blog Post
    ... In this episode of the Addgene Podcast, we introduce you to the Journal of Emerging Investigators, an...interview with the Journal of Emerging Investigators Podcast breakdown Intro Chapter 1 - Introduction to Mark...the audio engineer and producer for the Addgene Podcast.      Additional Resources from the Addgene Blog...

  2. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...a peer-reviewed manuscript OR a preprint. In the case of a preprint submission, we are happy to update...cloned into it. gRNAs are used for directing CRISPR/Cas9 to a targeted genomic location, while shRNAs are...format, e-mail us at  [email protected] Special cases Please contact us at [email protected] or +1 (...) if your deposit falls into one of the special cases below:   Pooled Library A pooled library is a ...

  3. 5 Science Rap Videos & Their Creator: Tom McFadden

    Type
    Blog Post
    ...with his new company, Science with Tom. In this podcast, we learn more about Tom and pick his brain for...the audio engineer and producer for the Addgene Podcast.     Additional Resources on the Addgene Blog ... a Grad Student Community Check out All of Our Podcasts Find Great Educational Content in our Plasmids...

  4. An Interview with Connie Cepko - Gene Therapy, Plasmid Tools and Insights to Success

    Type
    Blog Post
    ... School researcher Connie Cepko. Listen to the podcast below to hear all about the research being conducted...interview. Happy Listening! Click here to listen! Podcast breakdown: (Time : Topic) 0:00-1:00 : Introduction...the audio engineer and producer for the Addgene Podcast.     References 1.Tang, Jonathan C.Y., et al. “...

  5. Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana

    Type
    Blog Post
    ...In this episode of the Addgene Podcast, we sit down with Kwabena Duedu, a researcher at the University... to Ghanaian scientists Click here to listen! Podcast breakdown (Time : Topic) 0:00 - 7:11 : Introduction...the audio engineer and producer for the Addgene Podcast.     Additional Resources on the Addgene Blog ...

  6. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...endogenous levels (Laflamme et al., 2019). CRISPR-Cas9 approaches are commonly used to produce KO cell ...is not amenable to knockout approaches. In these cases, we would recommend a knockdown approach instead...lines based on expression of the target. In this case, you could compare the antibody’s labeling of the...Biddle, M. S., Smallwood, D. T., Weston, C. A., Castells, E., Bowman, V. W., McCarthy, J., Amrani, Y., ...

  7. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    ... lentiviral transfer vector used to deliver both Cas9 and your gRNA of interest to mammalian cells. It... blog post on Genome-wide Screening Using CRISPR/Cas9. If you need help cloning a gRNA into lentiCRISPR...References 1. Shalem, Ophir, et al. "Genome-scale CRISPR-Cas9 knockout screening in human cells." Science 343.6166...

  8. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    ... frequently used elements in plant expression cassettes: plant promoters, N-terminal tags, coding sequences...C-terminal tags, plant terminators and resistance cassettes for selection of transgenic plants. The complete... gives the option to stack several expression cassettes onto a single T-DNA in subsequent rounds, dramatically...

  9. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    ...have one associated with the gRNA), application, cas9 species, PubMed ID, and depositor. The CRISPR applications...nick, purify, tag, scaffold, CRISPR-display, and dCas9-FokI. This table is both sortable and searchable...alphabetically or in ascending or descending order, in the case of numeric columns.  The data in any column are ...

  10. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...Camelid family is composed of camels, llamas, and alpacas (and their undomesticated counterparts) - not exactly...soon after found similar antibodies in llamas and alpacas (Figure 1). At the time, researchers were already...started with immunizing a camelid (usually llamas or alpacas). But because sdAbs are so amenable to recombinant...

  11. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

    Type
    Blog Post
    ...at eScience Labs also write test question banks, case studies, and experiments for labs, in addition to...comprehensive exam proposals, a few PhD theses, and occasionally posting something science-related on Facebook...science education tools? Check out this Addgene podcast with educator and science rap mastermind Tom McFadden...

  12. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...simultaneous gene activation in plants.  FnCas12a, unlike other Cas12a nucleases identified, functions at temperatures...editing in moderate thermophiles.  These evolved SpCas9 variants have improved activity on NAG PAMS and...

  13. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...skill acquisition. Classes covered filmmaking, podcasting, philosophy of science, creative writing, designing...an animated video depicting nuclear fission; a podcast with a genetics researcher on parasitoid wasps;...the face of disparate spheres of research. In my case, an interest in neuroscience was carried over and...

  14. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    ...research. Image from Reproducibility for Everyone. Case study 1: Cell lines Many of the reagents used to...world shows that science is truly a global endeavor. Case study 2: Plasmids Another example of reagents that...Browse Addgene's COVID-19 plasmids and resources! Case study 3: Mouse strains Reagents developed during...

  16. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ...unmeasurable skills and productivity coming from that casual discussion on the way to the office or in the kitchen... flexible work schedule the more we realize how casual chatter is very much missed in the virtual world...widely used and could bring back a taste of that casual kitchen chatter, ultimately sparking new ideas....

  17. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    ...FAP - fluorogen activating protein - dL5** in this case), and 2) a fluorogen (the TAP - targeted and activated...activated photosensitizer - MG-2I in this case). The fluorogen is not fluorescent until it binds its protein...easy to watch development in real time.  In both cases, dL5** was tagged with mCerulean3 (mCer3) for visualization...

  19. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...depending on the vector and virus. Viral CRISPR-Cas9 tools have been engineered to introduce frameshift...be used to express proteins. Viral expression cassettes typically contain a promoter (inducible or constitutive... Therapy with AAV Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells ...

  20. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...in immunotherapy is the combination of the CRISPR/Cas9 methodology with T cell receptor engineering (Ref...using randomly integrating viral vectors, CRISPR/Cas9 genome editing allows targeted engineering of the.... "Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection." Nature 543.7643 (2017...
Showing: 381 - 400 of 716 results