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  1. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...genome. Typically, it is the sequences between and including the LTRs that are integrated into the host genome...internal promoter. An internal promoter must be included as the wildtype LTR promoter is no longer present...

  2. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...backbone to generate R-mAbs of different subclasses, including subclasses that were not produced by the original...expanded their recombinant affinity reagent tools to include nanobodies. At Addgene, we are excited to be part...

  3. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ...Others ran experiments at odd hours, used more secluded workspaces, or politely asked to not be disturbed... of making an error. Try a variety of things, including (and maybe especially) suggestions you are initially...

  4. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...generate antibodies against many types of molecules including proteins, lipids, and carbohydrates, in this blog...2005). This is reflected in the antibody’s cost. Conclusion Whether you choose to use a monoclonal antibody...

  5. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...shares many common features with gamma-retrovirus including genome architecture, host integration, and its...its cargo, in fact its viral life cycle does not include genomic integration. While the expression of adenovirus...

  6. Viral Vectors 101: Viral Applications

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    Blog Post
    ... tools with many applications. These libraries include sgRNAs targeting the entire genome, overexpression...several advantages over traditional vaccines, including 1) cellular responses in addition to antibody ...

  8. Behind the scenes: Addgene’s new search engine and more

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    Blog Post
    ...80,000+ reagents and 700+ educational resources — including blog posts, protocols, guides, and collections...we rolled out multiple changes to our website, including the recent homepage and search tool updates. Now...

  9. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...will it make them think? Does this lead to the conclusions I want them to draw and the perspective I want...scientists has formed around science communication, including the teams at Addgene, ComSciCon, and many more...

  10. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...be activated by an array of different ligands, including hormones, neurotransmitters, photons or odorants... new receptors were termed Designer Receptor Exclusively Activated by Designer Drugs (DREADDs) (Roth, ...

  11. Plasmids 101: Inducible Promoters

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    Blog Post
    ...Other examples of chemically induced promoters include positive inducible alcohol and steroid regulated... be induced by heat or cold exposure. Examples include the heat shock-inducible Hsp70 or Hsp90-derived...

  12. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...sociology, behavior, and artificial intelligence (including a team that created a novel form of AI that can...require large data sets. Whether these data sets include videos of behavior, many many images of brain regions...

  15. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...expression of other endonucleases, like Csy4, or the inclusion of processing signals, such at tRNAs, to process...its own pre-crRNA arrays eliminates the need to include multiple promoters to drive crRNA expression and...

  16. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ... DNA there, although other restriction sites (including other XbaI sites not preceded by GA or followed...either be difficult to interpret or lead you to conclude your plasmid is not correct! Conversely, enzymes...

  17. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...intact β-galactosidase produces pigment from x-gal (included in the transformation plate medium), turning the... colony blue. We should note that IPTG is also included in the medium to ensure transcription of the lac...

  18. Addgene: The First Twenty Years

    Type
    Blog Post
    ...we’ll be expanding our viral vectors service to include AAV Packaged on Request! “I love Addgene. I think...growing library of antibody educational resources, including our new Antibodies 101 eBook, the Antibody Guide...

  19. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...pillars for antibody validation.  The 5 pillars include genetic strategies that test expression in knock-out...conditions. Be prepared to troubleshoot and be sure to include proper positive and negative controls as you get...
Showing: 381 - 400 of 793 results