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Showing: 381 - 400 of 642 results
  1. Antibodies 101: Validation

    Type
    Blog Post
    ...Show suitability for an intended application - Remember, just because your antibody performs as expected...is just as helpful as knowing when it will. And remember, antibodies are like puppies. Just because an ...
  2. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...contributed by guest blogger, Addgene Advisory Board member, and Institute Scientist at the Broad Institute.... While this seems obvious, it is important to remember that the same is true when designing gRNAs for...
  3. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...commonly added to aid in protein purification. Just remember to remove the stop codon for C-terminal tags and...yeast expression vector that adds a SV40 NLS Myr Membrane localization pWZL-Neo-Myr-Flag-DEST - ...
  4. Luciferase Plasmid Collection

    Type
    Collection
    ...imaging and other applications. And see PalmGRET , a membrane-anchored version useful for labeling extracellular... Blog: Luminescence Content last reviewed: 26 September 2025 Do you have suggestions for other plasmids...
  5. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...ribonucleoprotein particles (RNPs) to both zebrafish embryos and the mouse inner ear. Fourth-generation cytosine...Tsang in August 2020 and by Emily P. Bentley in September 2025. References and Resources References Chen...
  6. Plan Your Experiment

    Type
    Collection
    ...read our three tips to improve HDR efficiency . Remember that HDR also requires donor DNA to serve as template...can transfect at high efficiency, such as human embryonic kidney 293 (HEK293) cells. For more difficult ...
  7. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...via genetically encoded proteins. PhoCl is a new member of this class that works via a different mechanism...ribonucleoprotein particles (RNPs) to both zebrafish embryos and the mouse inner ear. These exciting papers ...
  8. Genetic Code Expansion

    Type
    Collection
    ...concentration, and the cell lines used previously. Remember that the orthogonal pairs of aaRS and tRNA that...opens in a new window) Content last reviewed: 19 November 2025 Do you have suggestions for other plasmids...
  9. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    ...tagged with #DeckTheLab throughout the month of December are eligible to win a hot chocolate party that...
  10. Addgene's 20th Anniversary Party

    Type
    Blog Post
    ...! Executive Director Chonnettia Jones and Board Member Joanna Brownstein were also on the welcoming committee...
Showing: 381 - 400 of 642 results