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We narrowed to 802 results for: Eng

Showing: 381 - 400 of 802 results

  1. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...sources of information if those sources affirm or challenge their beliefs. I asked participants to read short...read an abstract for a study with findings that challenged their beliefs, they were much more likely to ...dissonance (Kunda, 1990). More specifically, they were engaging in a behaviour called the “biased evaluation of...

  2. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    ...newborn gut. One of Fatima’s ultimate goals is to engineer bacteria for the efficient product of HMOs that...surfaces. After incubating the sensor with bacteria engineered to produce HMOs, sensor binding results in an...future work with HMOs will enable researchers to engineer healthier microbiomes in people all over the world...

  3. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...seqWell, Inc., our mission is to overcome crucial challenges in NGS by developing technologies that can help...problem in multiplex NGS library preparation. The challenge here is that, as more samples are sequenced simultaneously...enhanced normalization of read count and fragment length across samples, NGS data obtained via plexWell™...

  4. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    ...Cofactors/Substrates Required Not Required Signal Strength Lower Higher Sensitivity Higher Lower Background..., substrate requirements and photon emission wavelengths. Renilla luciferase from the sea pansy (Renilla...these luciferases have been subjected to further engineering to improve upon the properties of the native ...

  5. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...many career pathways exist for scientists and engineers, a few brave scientists will choose to start their... interdisciplinary, with emphasis on genetic engineering, sequencing, and translation of results to companies....” Take home message Starting a lab can be a challenging process. Each PI emphasizes the importance of...

  6. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...fluorescent as well as FRET-based biosensors have been engineered to detect a wide array of neurotransmitters ranging...biosensors? Well, a lot of these sensors are already engineered into viral vectors, so if you want to use them...Biosensors for Neurotransmission and Neuromodulation: Engineering and Applications. Frontiers in Cellular Neuroscience...

  7. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...and tractable tools for genome engineering. Scientists have engineered viruses to target specific cell...infect. In the lab, this means viruses can be engineered to be cell type specific. Once inside the host...

  8. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    ...Non-Continuous Evolution (PANCE) selection systems to engineer better and faster ABEs (Fig. 2). These systems...ABE7.10. Find the ABE8e plasmids at Addgene! Why engineer and optimize base editors? Targeted evolution ...Lapinaite A, Newby GA, Thuronyi BW, Wilson C, Koblan LW, Zeng J, Bauer DE, Doudna JA, Liu DR (2020) Phage-assisted...

  9. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...pGGA008 (AlcA) and pGGC011 (AlcR) from red flame GreenGate kit Steroid-regulated LexA promoter XVE (synthetic...shock-inducible Cre and Cas9 for easy genome engineering in species like C. elegans and Drosophila. Light... induce transcription of your target gene? The strength of inducible promoters can vary a lot. If you’...

  10. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...reacting to a chemical, but by emitting a specific wavelength of light in response to excitation by a laser... degrading them. You’ll also need to know the wavelength of laser needed to excite them - and it’s a good...   Fig 2: Try to inversely pair fluorophore strength with protein abundance.    Second, you’ll ...

  11. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...Immunologists are coming together to tackle the challenge of curing cancer in cross-disciplinary projects...CRISPR/Cas9 methodology with T cell receptor engineering (Ref 5)  as described by Blue Flame Addgene depositor..., CRISPR/Cas9 genome editing allows targeted engineering of the TCR locus. He also highlighted the UCSF...

  12. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ... on differences in emission wavelength. Filtering the emission wavelength partially separates the signals...In fluorescent imaging, you use the excitation wavelength to specify which FP emission you’ll capture. ...

  13. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...this virus. While various Cas9 mutants have been engineered to perform unique editing tasks, classic Cas9...LentiCas9-blast is a Cas9 expression vector developed by Feng Zhang’s lab at the Broad Institute (Sanjana et al...makes generation of high titer Cas9 lentiviruses challenging. This cytotoxicity also causes growth rate variability...

  14. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...computation work comes with new sets of challenges. One challenge with computational analyses is to keep... are doing what you are doing. Another common challenge is to keep track of what works, and what does ...

  15. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...advantage to using the CRISPR/Cas system for genome engineering is the fact that Cas9 can be easily programmed...smegmatis (Yan et al., 2020). NHEJ can also be engaged by variants of the canonical Cas9 approach. A pair...UNC Chapel Hill.   References Bennardo N, Gunn A, Cheng A, Hasty P, Stark JM (2009) Limiting the Persistence...

  16. dTAG - You're it!

    Type
    Blog Post
    ... evaluate degradation of ENL. Cell lines were engineered to express ENL-FKBP12F36V and endogenous ENL ...evaluate degradation of MELK. Cell lines were engineered to express FKBP12F36V-MELK and endogenous MELK... evaluate degradation of YY1. Cell lines were engineered to express YY1-FKBP12F36V using CRISPR/Cas9-mediated...

  18. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...injected with an immunogen. This might be the full-length protein that you want to generate antibodies against...Otherwise, you might experience variability in the strength of staining and background noise. If you do use...control across experiments to be aware of signal strength. In general, polyclonal antibodies produce a higher...

  19. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...excitation wavelength. A fluorophore is less likely to bleach when it is excited off the peak wavelength, since... excitation powers are often used to reduce the length of the experiments. As the photobleaching rate ...

  20. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...with him.  He is also available for speaking engagements as are many of the coaches I have mentioned. ...Consulting works remotely, will travel for speaking engagements and has many areas of specialty from leadership...but the website looks interesting and it is in English and Spanish. She is based in Spain. The bottom ...
Showing: 381 - 400 of 802 results