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We narrowed to 1,145 results for: Gene

Showing: 381 - 400 of 1145 results

  1. Addgene at SfN23!

    Type
    Blog Post
    ... at Addgene's booth, #3422, in the nonprofits section.  The Booth What will you find at Addgene's booth...scientific curation manager, Angela, experts on the Addgene repository, are also attending! Finally, our team....  You'll also find tons of information about Addgene's ready-to-use antibodies, viral vectors, and, of...course, plasmids! Plus, we'll be giving away fun Addgene swag for the duration of the conference (be warned...

  2. Working 9-5: an Addgene Co-op

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    Blog Post
    ...intended. With the use of applications like SnapGene and Geneious, I was able to determine if any discrepancies...able to explore other parts of Addgene. This was done through the “Addgene 101” series, where each team ... Communication function at Addgene. Working a 9-5 As it turned out, Addgene uses something called “core...completing their co-op at Addgene. This is her second contribution to the Addgene blog.      More resources...brought me, a second year bioengineering student, to Addgene. I am currently working on the QC/QA team as a ...specific overview. It was immediately clear that Addgene wanted to ensure applicants understood what the...that the two hours were split up between several Addgene employees, who all made the daunting task feel ...

  3. Bioinformatics at Addgene

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    Blog Post
    .... More than half of these sequences were generated by Addgene as part of our QC process. Automation and...From the beginning, one of Addgene’s goals was to ensure the scientific community obtained high-quality...the years to keep up with the latest tools. In Addgene’s early days, we would ‘spot-check’ critical regions...sequencing at our facility using Illumina MiSeq Next Generation Sequencing (NGS) technology. As our repository...will describe the importance of bioinformatics at Addgene and how our search for solutions led us to a partnership...bioinformatics needs       Fig. 1: QC'ing data at Addgene is like standing under a Niagara Falls sized rainbow...our software, even with continuous updates. The generous donation of a Illumina MiSeq sequencer enabled...

  4. Your Top Requested Plasmid in 2015!

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    Blog Post
    ...to knock-out single genes in clonal populations and to knock-out thousands of genes in mixed population...targeting over 19,000 and 20,000 human and mouse genes respectively, use lentiCRISPR v2 as the backbone...Resources on the Addgene Blog Browse Our CRISPR Posts Learn about Using CRISPR to Generate Knockouts Read...announce that the plasmid most requested from the Addgene repository in 2015 was... lentiCRISPR V2   LentiCRISPR...Availability and Use LentiCRISPR v2 is available from Addgene both as a single empty vector and in pooled libraries...reagents available to the research community through Addgene and look forward to another great year of sharing... Plasmids to Mammalian Cells Resources on the Addgene Website Visit our Lentiviral Guide Pages Find ...

  5. CRISPR in the Clinic

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    Blog Post
    ... J.A. Engineering the next-generation of CAR T-cells with CRISPR-Cas9 gene editing. Mol Cancer 21, 78 ...dominant cataract disorder caused by a single copy of a gene known as Crygc (Wu et al). They found that the cataracts...able to demonstrate functional correction of the gene in clonally expanded organoids. This work, and many...immunotherapy for cancer treatment by traditional gene therapy pipelines (viral), and researchers are currently...single nucleotide polymorphisms (SNPs) in the TTR gene and is molecularly characterized by misfolding of...blog post on CRISPR’s potential for correcting monogenetic diseases. Now, almost 10 years later, CRISPR’...Resources References Yuxuan Wu et al. "Correction of a Genetic Disease in Mouse Via Use of CRISPR/Cas9." Cell ...

  6. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ... at Addgene 2. Green, Alexander A., et al. "Toehold switches: de-novo-designed regulators of gene expression...was an in vitro paper-based platform that allowed gene circuits to be deployed out of the laboratory in...combined technologies, we built paper-based synthetic gene networks for the detection of antibiotic resistance...-of-concept work was exciting because it enabled gene circuit-based sensors to be used outside of the ... 3. Pardee, Keith, et al. "Paper-based synthetic gene networks." Cell 159.4 (2014): 940-954. PubMed PMID...Sensor Constructs at Addgene As we report, we have embedded this next generation of toehold switch-based...corresponding portions of the Zika virus genome with Addgene to facilitate rapid sharing of these tools with...

  7. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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    Blog Post
    ...permanently knock out or knock-in a gene? Do you want to activate or repress gene expression? Are you trying to...reporter gene (e.g. GFP) to identify and enrich positive cells or a selection marker to generate stable ...conjunction with activator or repressor domains to control gene expression. Base editors can help you make precise... separate transfer vectors. May contain reporter gene (e.g. GFP) to identify and enrich positive cells... for each, and potential delivery methods.   General considerations for planning your CRISPR experiment...Wildtype Cas9 or Cas9 nickase are appropriate for generating knockouts or knock-ins, or introducing mutations...PCR followed by gel electrophoresis and/or next-generation sequencing more appropriate? If you already...

  8. 22 Hot Plasmid Technologies from 2014

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    Blog Post
    ...effectively targets your gene/region of interest is a key step for CRISPR/Cas9 gene editing. Dr. Masahito...sequence or gene of interest into the pCoofy vectors, select the appropriate pair of vector and gene primers...vector and gene primer for SLIC cloning. Amplify the pCoofy vector and the sequence of gene of interest... can be used to generate random mutagenesis libraries or to insert heterologous genes, reporters, or other...Updated mini-transposon vector for bacterial mutagenesis or gene targeting Victor de Lorenzo's lab has...approach to genetic engineering which drastically reduces the time and effort needed to insert new genes into...successfully create GFP fusion proteins with a variety of genes across the genome. Although this tool was published...

  9. AAV for Neuronal Tracing

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    Collection
    ...G-deleted rabies lacks the viral glycoprotein (G) gene, which is not required for the transcription or .... Since this starter cell now has all the viral genes present, the virus can spread from this cell to ...Ready-to-use AAV available from Addgene's viral service encoding tools for monosynpatic retrograde neuronal...to perform retrograde neuronal tracing. Note : Addgene does not distribute rabies virus. AAV Vectors for... was modified so that it could only infect a genetically specified neuronal population. This is done using... give rise to G-protein coated RAVB. Resources Addgene Blog: Rabies and Neuronal Tracing References Wickersham...restriction of transsynaptic tracing from single, genetically targeted neurons. Neuron. 53(5):639-47. PMID:...

  10. Identifying Sequence Elements with SnapGene's Feature Database

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    Blog Post
    ... extensive heterogeneity in the feature sequences due to genetic drift and the use of genes from different...raw plasmid sequence and display frequently used genes and control elements. Development of this tool required...sequence feature that may be used to make fusion genes, detection needs to occur even if one or two codons...of the feature. With the increasing popularity of gene synthesis, many researchers now use codon-optimized... who uses SnapGene or the free SnapGene Viewer companion product. If you don’t use SnapGene yet, you can...Resources on the Addgene Blog Read about our SnapGene powered plasmid maps Learn how we use SnapGene during the...sequencing at Addgene Additional Resources on Addgene.org Example plasmid analysis powered by SnapGene Browse...

  11. Plasmids 101: Origin of Replication

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    Blog Post
    ...Now that we know all about antibiotic resistance genes, let’s consider another basic element of any plasmid...Do you want to make a lot of your plasmid? Is the gene toxic in high amounts? It is always good to keep...below for additional considerations. *Requires pir gene for replication (reference). **F1 is a phage-derived...copies of plasmids if they contain large inserts or genes that create a toxic product. The E. coli strain...survive within cells. The replicons of plasmids are generally different from the those used to replicate the... to make additional copies. ori sequences are generally high in As and Ts. Why, you ask? Well, A-T base...few copies depending on how they are regulated. Generally, control of replication is referred to as "relaxed...

  12. TALEN Plasmids and Kits

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    Collection
    ... order to generate TALORs (TAL Orthongal Repressors) that can be used to custom repress gene expression... successfully been employed for deletion mutagenesis and gene targeting in human and mouse pluripotent...editing was also shown to alter regulation of nearby gene expression. The vectors listed below have the EF1α...kits and accessory plasmids available from Addgene for generating your own specific TALE plasmids. Genome... TALEN Plasmids and Kits You may also like... Addgene's Genome Engineering Guide CRISPR Plasmids Zinc ...amino acid repeats in the TAL effectors. View Addgene's TALEN Guide. These collections and accompanying...Takashi Yamamoto Additional Plasmids for Use with Addgene TALEN Kits For Use With: ID Plasmid Depositing ...

  13. QC Sequencing Technologies at Addgene

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    Blog Post
    ...containing cassettes of the same gene multiple times.  Bioinformatics at Addgene After a run on our Illumina...Resources on the Addgene Blog A Look at Addgene’s QC Process Bioinformatics at Addgene The Power Behind...Previously, we provided a general overview of the QC process at Addgene. All plasmids go through this...technologies that Addgene uses in the QC process, when these technologies are used, and how Addgene keeps up with...Sequencing technologies used at Addgene Sanger sequencing (“First-generation sequencing”)  Sanger sequencing...assembles the raw data.  Addgene’s de novo assembly pipeline usually generates the correct plasmid sequence...revisit the assembly in a program called Geneious. Within Geneious, the raw FASTQ reads are used for either...

  14. A Look at Addgene's QC Process

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    Blog Post
    ...plasmid to ensure our assembly result is accurate The gene/insert or any major feature highlighted by the deposited...sequenced by Addgene’s QC team. Email help@addgene.org to get started! Addgene’s QC process Addgene prioritizes...deposited sequence(s) in SnapGene. SnapGene annotates the plasmid maps based on SnapGene’s “Common Feature” library...Quality Control Now at Addgene Bioinformatics at Addgene Resources on Addgene.org Educational Resources... Figure 2. Addgene’s basic QC process. Created with BioRender.com. Once Addgene receives deposited...receiving the replacement plasmid at Addgene. QC is a team effort Addgene’s QC team is full of dedicated scientists... Resources Additional resources on the Addgene blog Addgene's Tips for Plasmid Quality Control Plasmids...

  15. Addgene's Website Update

    Type
    Blog Post
    ...do scroll down? You’ll find Blugene, as well as information about Addgene, our mission, links to our deposit...improving the tools you use to share and access them. Addgene’s product, development, and content teams have made... made some changes to the look and feel of addgene.org. Some you’ll notice right away, while others may...resources, tools, collections in our catalog, and the Addgene Help Center. A big part of this redesign was pulling... place, so you can quickly navigate around the Addgene website without scrolling up and down the page....skim through. Users with disabilities will find Addgene’s website easier to navigate, thanks to design choices...— check out all the new features and designs addgene.org! And when you’re finished, feel free to take ...

  16. Viral Production at Addgene

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    Blog Post
    ... plasmid encoding rep and serotype-specific cap genes, and a plasmid encoding adenoviral helper sequences...viral vectors are tested in vitro and in vivo for gene expression and/or function. These data are sometimes...copies of the lentiviral Rev responsive element (RRE) gene and the cellular ribonuclease P/MRP 30 kDa subunit...vector genome titers by droplet digital PCR. Human Gene Therapy Methods, 25(2), Article 2. https://doi.org...Titer by a Novel Droplet Digital PCR Method. Human Gene Therapy Methods, 29(2), 96–103. https://doi.org/...Production AAV distributed by Addgene has been produced either in-house by Addgene scientists or through collaboration...either performed by Addgene or by the University of Pennsylvania Vector Core. In general, titering is performed...

  17. Summer Fun at Addgene!

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    Blog Post
    ...means summer is right around the corner. Here at Addgene, we’re always inspired by the new seasons, so we've...complex questions, and giving tips for consistently generating solid data. If you’re ready for the next steps...We’re very excited to be launching the official Addgene TikTok at the end of this month! We’ll be sharing...lab, along with some behind-the-scenes peaks of Addgene’s people, culture, and workplace. Rumor has it that...

  18. Lab Automation at Addgene

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    Blog Post
    ...mail due to laws concerning genetically modified organisms. Since Addgene is very proud to help scientists...times easier. We’ve certainly felt this way at Addgene so we’ve been putting some work into finding robots... many more robots than you ever knew existed! Addgene recently started automating a few protocols to ... hour. Automating vial fiIling The majority of Addgene plasmids are shipped as agar stabs in glass vials... easily programmed for a variety of lab needs. Addgene uses it to fill as many vials as it can in the ...Label your tubes with a label maker. Label makers generate far more legible labels than your hand and help...avoid sore fingers. There are other process that Addgene would love to move forward and automate. For instance...

  19. Targeted Mutagenesis with EvolvR

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    Blog Post
    ...targeting EvolvR to five dispersed regions of the target gene of spectinomycin, rpsE. These results not only uncovered...directed mutagenesis to generate specific mutations in your plasmids Use REPLACER Mutagenesis to mutate...and forward genetics harnesses this at a large scale by screening diverse libraries of genetic variants....Berkeley, provides a new approach for generating novel genetic variants in bacteria. Read on to learn about...EvolvR’s on-target mutagenesis rate was 2.5 x 10-6 mutations per nucleotide per generation vs. 10 x 10-10 ...additional mutations to PolI3M generated PolI5M which increased EvolvR’s mutagenesis rate to ~10-3 mutations ...than one genomic site for mutagenesis, a feature which they used to generate bacteria resistant to both...

  20. Countdown to Halloween @Addgene

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    Blog Post
    ...Halloween is only 2 days away and here at Addgene we couldn’t be more excited! As a small, tight-knit...email system.  Our 2013 winners dressed up as Addgene's favorite lunch... a BonMe noodle meal, crammed...interested in finding out who wins this year’s Addgene costume contest, like us on Facebook and checkout...  addgene...
Showing: 381 - 400 of 1145 results