We narrowed to 918 results for: IRE

...resulting from past practices and mores.  His list is dire:  The current system is unsustainable, hypercompetition... tackle the issues with rules and practices of indirect costs.  The peer review and grant awards systems... encouragement.  Dr. Sibby Anderson-Thompkins, Director of the Office of Postdoctoral Affairs at UNC, ... put on good mentoring and applauded the new requirement for PIs to include in their annual NIH progress...

...the recognition domains—including the CTD and the entire REC lobe—do not structurally resemble any other...weak, random binding to precision gene editing requires three major steps: RNA loading, DNA sequence recognition...The RNA guide sequence is extended as a single unpaired strand in the center of the protein. The repeat...1, nestled between the REC and CTD domains, is required for Cas9 nuclease function. Stem loops 2 and 3...But how to test DNA bases that are already snugly paired to each other? At this point, the protein can switch...

...disease” often requires understanding the disease genetics and the “treatment” usually requires drugs. While...validated for specificity to their target, or their direct target can be identified if it isn’t known. If ...undruggable’ proteins – having multiple hits or an entire pathway of possibilities greatly increases the ...

...sequence is much more forgiving and can be mostly or entirely replaced with DNA bases while retaining activity...specifically recognizes its folds, especially the repeat:antirepeat duplex. Cas12 recognizes the 2’-OH of RNA ...However, this result was unusual. Optimization is required for best results. Different targets benefit most...starting points. Hybrid guides only reduce certain undesired outcomes. Off-target editing of mismatched genomic...

...time spent at the desk, but it is worth it. This requires a focus on deliverables. It does not mean a manager... and colleagues Sometimes this means you have to fire a good performer if they are enough of a jerk to... of most people, not just scientists) Are self-directed, but need precise leadership and support from ...Scientists. Subscribe to the Addgene Blog to follow the entire series as it is published. Good introductory reference...

...this awesome experiment you want to do, but it requires some AAV. You’ve never worked with AAV before,...plasmids have a promoter, but this element isn’t required when the transfer plasmid harbors non-coding sequences...’s talk about some of the elements that aren’t required parts of an AAV transfer plasmid, but are often... control transgene expression. Lox sites are directional 34 bp sequences that can flank the transgene ...

...tagmentation preparation approaches described previously require a preliminary double stranding step. The double...single-stranded and both TA-based ligation and tagmentation require a double-stranded template.  Using viral genome...of serotype confirmation, this method does not require any additional reagents or lab work (Pacouret, ...polynucleotide chains. J Virol 9(2): 394-396. Lecomte E, Tournaire B, Cogné B, Dupont J-B, Lindenbaum P, Martin-Fontaine...

... my curiosity did not ultimately translate to a desire to do the research itself. Several years of exclusively...more creative faculties into motion, such that a desire to once again write for fun eventually saw an English...remarking to a classmate that, if this was one day required of me for work, I would have a high level of career...neurological health risk. Having played the sport my entire life, I was curious as to what the research had...

...wide variety of applications. But some techniques require you to select against the presence of a transgene... Antibiotic selection would only then kill the desired plants! PCR or fluorescent markers are currently...plants, but screening these can be challenging and requires specialized equipment. Reporters for studying ...from these plants will be green, containing the desired mutation but lacking both RUBY and the construct...

...and varying methods of replication. In fact, an entire classification scheme, known as the Baltimore replication...follow central dogma (gDNA to RNA to proteins) or require an intermediary DNA replication step between DNA...virus. RNA genomes can be transcribed into mRNA, directly translated into proteins, or, in the case of retroviruses...reproduce when experimentally necessary, genes for the required proteins are packaged in separate plasmids and...

...choosing a FP, but much of the primary data one might require when making such a decision remains spread across...to us for advice on choosing a protein, we often direct them there as a starting point.  However, there...previous versions.  Currently, all submissions also require a DOI referencing primary literature, and every...information on a particular FP; this can be done directly using the search bar on the homepage (and at the...

...frequency. Although highly useful, this technique requires extensive sample processing to avoid disrupting...appear red. To enable this color switching, TRICK requires the following components: a GFP protein that ...field of view for constant tracking. You can then directly monitor multiple rounds of translation from this...caveats. In both cases, creating a reporter mRNA requires inserting multiple hairpin sequences into the ...

...researcher at the Wyss Institute for Biologically Inspired Engineering and member of the Addgene Advisory...production frustrating - a researcher can complete an entire lentiviral production protocol only to find that...widely requested and incredibly useful tools, they require extensive background work from the requesting scientist...Church lab at the Wyss Institute for Biologically Inspired Engineering and the Zhang and Doench/Root labs...

... favorite books - both scientific and not - to inspire you to read a little more this October. Science...science of race."     - Joanne Kamens, Executive Director  I Contain Multitudes by Ed Yong "Microbes are...another secret."     - Marcy Patrick, Associate Director of Quality Control Jonathan Strange & Mr. Norrell...Club (ABC) founder, Eric Perkins, is Addgene’s Director of Science Product Management and explains the...

...is not enough on its own to help you find your desired plasmid. In many cases, you will need a screening...you will need to sort through them to find the desired clone. Do your selection, then screen Why add a...what about identifying clones that contain your desired insert? This is where a  screen comes in. Let’s...process. This way, we can confirm the sequence of the entire plasmid. Please note that NGS verification is more...

...ancient Chinese game Go). In addition, there was an entire story-telling session organized by Story Collider...in neurobiology (and really in all of biology) require large data sets. Whether these data sets include...or sequences of microbial DNA they all seem to require some mathematical know-how for analysis. If you...from Addgene. While super useful, these vectors require a bit of expertise and can be difficult for individual...

...students in real-world lab activities? I’m trying to inspire them and also keep up with all the new biotechnology...being asked in MIT’s research labs, BioBuilder inspires students and teachers to apply engineering principles...breathalyzer to pesticide breakdown, production of fire-resistant housing insulation, and cleaning up salt...BioBuilder's open-access curriculum available on Amazon Inspire.    Many thanks to our guest blogger, Mary Tamer...

...(FP) require external light activation, you can’t use fluorescence to monitor processes directly affected...luminescent imaging: A luminescent substrate is required for imaging, and it is important to verify that...technology. Multicolor imaging using Nano-lanterns requires a linear unmixing algorithm to separate the various...

...blessed to have an incredibly supportive Board of Directors at Addgene. Our Board helps keep our focus on ...best practices and encouraging us to explore new directions. I would like to thank them for their time, dedication...can afford to do and when. Addgene’s Legal Team acquired permission from relevant plasmid depositors and...proud to be adding viral vector services to our repertoire. Additional Resources on the Addgene Blog ...

... nucleus through nuclear pores and thus do not require breakdown of the nuclear envelope during cell division...gamma-retrovirus are so closely related, their required components are very similar. While plasmids containing...specific cell type doesn’t always lend itself to direct application of virus. However, the cargo capacity...infect dividing and non-dividing cells and doesn’t require genomic integration to express its cargo, in fact...