We narrowed to 1,024 results for: LEA

...the sample, either by chemical treatment or photobleaching or by stripping out the antibodies, then start... (magenta), anti-AnkyrinG (green), and Hoechst nuclear stain (cyan). Panels C1-C3 show magnified detail... et al., 2021) for more guidelines for creating clear and informative figures. Pro-tip! If you find yourself...antibody, it should get completely washed away and leave no signal. If you see lots of extra signal in these...have questions or any favorite tips and tricks, leave a comment below. Happy imaging! References and ...Wissink, E. M., & Weissgerber, T. L. (2021). Creating clear and informative image-based figures for scientific...

...world After learning about using sound to visualize bacteria in the lab, I was excited to learn about creative...Fluorescent Protein Resources and get started!  I learned a lot about creative ways to do science communication...communication and outreach - if you'd like to learn more about Science communication check out these SciComm...CRISPR Methods for Bacterial Genome Engineering Learn about Science Communication using Twitter  Resources...

...technology, the ability to direct enzymes – from nucleases to transcription factors – to specific sequences...a cell. Thanks to the work of Huimin Zhao and colleagues, we now have just the tool…designer PUF proteins...possibilities with PUF proteins The tool she and her colleagues led by Zhao described and demonstrated in a report...notes that for reasons that are not yet entirely clear some PUF proteins lose their solubility. Her lab...This isn’t the final version,” she said. “If we learn how to make the library even better, we would be...

...directly with one another – not just with the team leader. There is no way a manager can be efficient without...Harvard Medical School researcher Connie Cepko to learn about her management style.   Seeking feedback frequently...feedback and so receives this valuable input from at least some employees. Watch out for "information allies...feedback, but shouldn't be the only way.   From a colleague: MBWA "Manage By Walking Around" – don't hide ...Method. P = demonstrate patience, pause; A = ask at least one question to clarify the situation; C = confirm...

...continuity - two lead organizers on each program, one junior and one senior.  To learn more about the STIN's...generation of science communicators. In this chapter we learn about some of their events - science seminars, science...achieving success. Chapter 9: Science by the Pint - We learn why a "Science by the Pint" event is the best way...about starting your own SciComm organization and to learn about some of SITN's other initiatives, email sitnboston...

...productive relationships. I will be sharing what I’ve learned along the way in this ongoing series of blog posts...Harvard Medical School researcher Connie Cepko to learn about her mentoring style. Top 7 questions scientists...well as) their own.” “Someone to act as your ‘cheerleader’ – inspire you.” “Also a mentor can give you ...reach your goals Listens well and communicates clearly and openly Gives honest feedback without ever being... of, and to respect my students as developing colleagues even as I reinforce the necessary power dynamic...

...Northeastern University, you are expected to spend at least one semester (the majority spend two to three) working...much less specific overview. It was immediately clear that Addgene wanted to ensure applicants understood...in a brief fifteen minute interview. I remember leaving the interview hopeful to continue on in the process...spent a lot of time shadowing members of my team to learn what I was to do for my time at Addgene. This training...not been the case for me at Addgene. There is a clear effort to make sure people feel connected.    As...

... two labs through the BeHEARD Award. Read on to learn how plasmids from Addgene will be used to accelerate...the molecular mechanisms behind the disease are unclear. Dr. Di Schiavi plans on using C. elegans as a ...treatments for peritoneal mesothelioma. Dr. Nabavi and colleagues have compiled data from primary peritoneal mesothelioma...validate these candidate genes and allow Nabavi and colleagues to prioritize which genes they will use to develop...deserve a voice. Check back with Addgene often to learn of future rare disease research funding opportunities...

...that you could meet with learning (scientists are trained to be good at learning new things—this is a strength...specifically in the position. We frequently receive clearly generic cover letters that expound on bench skills...experience. Most Addgene PhD level job descriptions clearly describe that the roles do not involve bench research...relatives to network your way to a hiring manager. Learn to confidently describe your strengths in your resume...

...to maximize productivity and success? We asked leading professors for their top 3 tips. Tom Ellis 1....fluke.” 3. Engage in various projects and help colleagues with their work. “Your one project may be awesome... Ellis recommends that a student interested in leaving academia should present their research at conferences...students to have: working to deadlines, writing up clear and attractive reports, being good at presentations...connected to scientists and use the skills they learned during their studies. They suggest that you make...

...Deubner et al., 2019). For example, Taylor and colleagues used rAAV FLEx-based vectors to study the neural...). Not so long ago, however, it was completely unclear which subpopulation of DA neurons could activate...aversive stimuli. In a recent paper, de Jong and colleagues used rAAV FLEx-based vectors to answer this question...containing FLEx technology. Read the genOway press release for more information. References Abdallah, Khaled...hippocampal interneurons that express corticotropin‐releasing hormone." Hippocampus 26.1 (2016): 41-53. PubMed...PMCID: PMC4166436. Resources on the Addgene blog Learn more about FLEx vectors Download our Plasmids 101...

...complex minus its nuclease component, Cas3, while Type V-K CAST systems use a nuclease-deficient Cas12k..., Tn7-like transposons captured and repurposed nuclease-deficient CRISPR-Cas systems to help them spread...Gelsinger et al., 2024). In either type of CAST, the nuclease-deficient CRISPR-Cas system identifies and binds...barely effective at editing eukaryotic cells — at least, until they met the directed evolution expertise...

... vs. SpCas9, see the table below and read on to learn more about GeoCas9.   SpCas9 GeoCas9 Size 1368...ranging from 25-80 °C. Activity is expressed as Kcleave (min-1). SpCas9 has maximum activity from 35-45...experiments tested the ability of GeoCas9 RNPs to cleave substrate when the reaction was carried out at ...either 37 °C or 4 °C. Substrate was then added and cleavage product analyzed on a gel. SpCas9 is stable when... 30% human plasma. The discovery of GeoCas9 clears the way for CRISPR/Cas9 genome editing in facultative...

... Addgene blog Learn about Gateway cloning Read about genome engineering in flies Learn more about controlling...HDVR (2X Hepatitis Delta Virus Ribozyme), a self-cleaving ribozyme. In the second-generation SPARC2 system...HDVR is placed upstream of the second attP site to cleave and inactivate any read-through transcripts of ... efficiency of recombination and preferentially leads to gene constructs where the stop cassette remains...

...awareness of FOP. Please consider running with us or making a donation via the link below. Please continue to...being with the company for about six months, I learned there was a Genzyme-based running team called Running...Second, Addgene will be hosting the AJ’s Boston League team (Contributing many new heroes to the team ...years. Additional Resources on the Addgene Blog Learn about Addgene's Previous Work with Rare Disease ...

...engineering from ZFNs through TALENs and CRISPR-Cas nucleases, provided important context for the rest of the...reagents so freely with the academic community has clearly made a huge impact on this field. Advances in ...described a chimeric Cas9 that is improving the nuclease specificity dramatically and reducing off-target... service questions for nearly 7 years, and my colleagues and I have an internal motto for scientists who..., and I found myself extremely grateful to my colleague Jason for choosing Simon Singh's "The Code Book...

...Figure 3: When a bison wanders into the campsite, leave your samples behind! Paul and Anastasia (Potanina...hyperthermostable Cas9 This year it was wonderful to learn that one project led by postdoc Dr. Stephanie Schmidt... IgnaviCas9 is a hyperthermostable Cas9 CRISPR nuclease that is active up to 100°C.  The IgnaviCas9 protein...for long time periods and workflows where CRISPR nuclease activity is desired and time or protocol steps...useful. Additional resources on the Addgene blog Learn about other Cas proteins Read all of our CRISPR ...

...structure-guided direct evolution, Nathan Shaner and colleagues from The Scintillon Institute, Allele Biotechnology...Clavularia sp. protein CFP484. Hui-wang Ai and colleagues from the University of Alberta and University... in a 2.6-fold increase in signal relative to mCerulean imaged with a standard CFP set. mTFP1 can be used...would like to share with the scientific community, please deposit the plasmids at Addgene! References Ai... to the Addgene blog Resources on Addgene.org Learn about the Michael Davidson fluorescent protein collection...

...Additional resources on Addgene.org Learn about Addgene's viral service Learn about viral production Find viral...incorporate into their own labs. This week we are pleased to announce the submission of the results of our... cells (Vandenberghe, 2010). Rather than being released into the cell culture supernatant, the viral particles..., Luk H., et al. "Efficient serotype-dependent release of functional vector into the culture medium during...

...ever like to write about a recent plasmid deposit please sign up here.   Listen this episode of Hot Plasmids...contain a smaller RNA tag, are less prone to photobleaching, and achieve better signal in vivo as compared...increasing the brightness. Arne Fabritius and colleagues started with the FP mNeptune684 (originally described... rapid quantification of base-editing and Cas9 cleavage activity Article contributed by Angela Kaczmarczyk...show that the ACE reporter can also quantify Cas9 cleavage, thus exhibiting its versatile utility in quantifying...