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We narrowed to 1,019 results for: LEA

Showing: 381 - 400 of 1019 results

  1. Plasmid Grant to Aid Cervical Cancer Screening in Ghana

    Type
    Blog Post
    ...Blog Learn How We Raised Money for Rare Disease at the Superhero 5k Find New Cancer Resources Learn About...wrote in her application, “Cervical Cancer is the leading cause of cancer related morbidity and death among...researchers who need them the most. Check back here to learn more about future awardees and their inspiring research...Resources on Addgene.org Check out our Cancer Pages Learn about Global Plasmid Distribution ...

  2. Making Career Development an Integral Part of the Science Training System

    Type
    Blog Post
    ... trainer” programs.  Please join us for the pd|hub Workshop Report Public Release Event scheduled for ...student or postdoc ends up in a lab where they can learn the plus skills needed to succeed on the career ...project and the findings were captured in this just released report “Enhancing Dissemination of Evidence-Based...for March 4th from 2:30-4:00 pm ET.  You can learn more about the report's recommendations and join with...

  3. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...only a small number of glutamate molecules are released in each synaptic event and they are rapidly taken...development and cancer. But there is still much to learn about their myriad functions and therapeutic potential...Institute for Protein Innovation (IPI) recently released a set of highly-specific recombinant antibodies...2025). In retrotransposon insertion, a combined endonuclease-reverse transcriptase uses homology arms flanking...Shilatifard Lab created a very quick and efficient way to learn more about gene regulation. Find the TurboCas plasmid...solution. Recently, the labs of Gord Fishell and colleagues characterized a series of new enhancer-AAV vectors...

  4. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...the sample, either by chemical treatment or photobleaching or by stripping out the antibodies, then start... (magenta), anti-AnkyrinG (green), and Hoechst nuclear stain (cyan). Panels C1-C3 show magnified detail... et al., 2021) for more guidelines for creating clear and informative figures. Pro-tip! If you find yourself...antibody, it should get completely washed away and leave no signal. If you see lots of extra signal in these...have questions or any favorite tips and tricks, leave a comment below. Happy imaging! References and ...Wissink, E. M., & Weissgerber, T. L. (2021). Creating clear and informative image-based figures for scientific...

  5. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...world After learning about using sound to visualize bacteria in the lab, I was excited to learn about creative...Fluorescent Protein Resources and get started!  I learned a lot about creative ways to do science communication...communication and outreach - if you'd like to learn more about Science communication check out these SciComm...CRISPR Methods for Bacterial Genome Engineering Learn about Science Communication using Twitter  Resources...

  6. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ...technology, the ability to direct enzymes – from nucleases to transcription factors – to specific sequences...a cell. Thanks to the work of Huimin Zhao and colleagues, we now have just the tool…designer PUF proteins...possibilities with PUF proteins The tool she and her colleagues led by Zhao described and demonstrated in a report...notes that for reasons that are not yet entirely clear some PUF proteins lose their solubility. Her lab...This isn’t the final version,” she said. “If we learn how to make the library even better, we would be...

  7. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    ...directly with one another – not just with the team leader. There is no way a manager can be efficient without...Harvard Medical School researcher Connie Cepko to learn about her management style.   Seeking feedback frequently...feedback and so receives this valuable input from at least some employees. Watch out for "information allies...feedback, but shouldn't be the only way.   From a colleague: MBWA "Manage By Walking Around" – don't hide ...Method. P = demonstrate patience, pause; A = ask at least one question to clarify the situation; C = confirm...

  8. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    ...continuity - two lead organizers on each program, one junior and one senior.  To learn more about the STIN's...generation of science communicators. In this chapter we learn about some of their events - science seminars, science...achieving success. Chapter 9: Science by the Pint - We learn why a "Science by the Pint" event is the best way...about starting your own SciComm organization and to learn about some of SITN's other initiatives, email sitnboston...

  9. "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

    Type
    Blog Post
    ...productive relationships. I will be sharing what I’ve learned along the way in this ongoing series of blog posts...Harvard Medical School researcher Connie Cepko to learn about her mentoring style. Top 7 questions scientists...well as) their own.” “Someone to act as your ‘cheerleader’ – inspire you.” “Also a mentor can give you ...reach your goals Listens well and communicates clearly and openly Gives honest feedback without ever being... of, and to respect my students as developing colleagues even as I reinforce the necessary power dynamic...

  10. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...Northeastern University, you are expected to spend at least one semester (the majority spend two to three) working...much less specific overview. It was immediately clear that Addgene wanted to ensure applicants understood...in a brief fifteen minute interview. I remember leaving the interview hopeful to continue on in the process...spent a lot of time shadowing members of my team to learn what I was to do for my time at Addgene. This training...not been the case for me at Addgene. There is a clear effort to make sure people feel connected.    As...

  11. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    ... two labs through the BeHEARD Award. Read on to learn how plasmids from Addgene will be used to accelerate...the molecular mechanisms behind the disease are unclear. Dr. Di Schiavi plans on using C. elegans as a ...treatments for peritoneal mesothelioma. Dr. Nabavi and colleagues have compiled data from primary peritoneal mesothelioma...validate these candidate genes and allow Nabavi and colleagues to prioritize which genes they will use to develop...deserve a voice. Check back with Addgene often to learn of future rare disease research funding opportunities...

  12. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...that you could meet with learning (scientists are trained to be good at learning new things—this is a strength...specifically in the position. We frequently receive clearly generic cover letters that expound on bench skills...experience. Most Addgene PhD level job descriptions clearly describe that the roles do not involve bench research...relatives to network your way to a hiring manager. Learn to confidently describe your strengths in your resume...

  13. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ...to maximize productivity and success? We asked leading professors for their top 3 tips. Tom Ellis 1....fluke.” 3. Engage in various projects and help colleagues with their work. “Your one project may be awesome... Ellis recommends that a student interested in leaving academia should present their research at conferences...students to have: working to deadlines, writing up clear and attractive reports, being good at presentations...connected to scientists and use the skills they learned during their studies. They suggest that you make...

  14. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ...Deubner et al., 2019). For example, Taylor and colleagues used rAAV FLEx-based vectors to study the neural...). Not so long ago, however, it was completely unclear which subpopulation of DA neurons could activate...aversive stimuli. In a recent paper, de Jong and colleagues used rAAV FLEx-based vectors to answer this question...containing FLEx technology. Read the genOway press release for more information. References Abdallah, Khaled...hippocampal interneurons that express corticotropin‐releasing hormone." Hippocampus 26.1 (2016): 41-53. PubMed...PMCID: PMC4166436. Resources on the Addgene blog Learn more about FLEx vectors Download our Plasmids 101...

  15. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    ... Addgene blog Learn about Gateway cloning Read about genome engineering in flies Learn more about controlling...HDVR (2X Hepatitis Delta Virus Ribozyme), a self-cleaving ribozyme. In the second-generation SPARC2 system...HDVR is placed upstream of the second attP site to cleave and inactivate any read-through transcripts of ... efficiency of recombination and preferentially leads to gene constructs where the stop cassette remains...

  16. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ...complex minus its nuclease component, Cas3, while Type V-K CAST systems use a nuclease-deficient Cas12k..., Tn7-like transposons captured and repurposed nuclease-deficient CRISPR-Cas systems to help them spread...Gelsinger et al., 2024). In either type of CAST, the nuclease-deficient CRISPR-Cas system identifies and binds...barely effective at editing eukaryotic cells — at least, until they met the directed evolution expertise...

  17. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    ... vs. SpCas9, see the table below and read on to learn more about GeoCas9.   SpCas9 GeoCas9 Size 1368...ranging from 25-80 °C. Activity is expressed as Kcleave (min-1). SpCas9 has maximum activity from 35-45...experiments tested the ability of GeoCas9 RNPs to cleave substrate when the reaction was carried out at ...either 37 °C or 4 °C. Substrate was then added and cleavage product analyzed on a gel. SpCas9 is stable when... 30% human plasma. The discovery of GeoCas9 clears the way for CRISPR/Cas9 genome editing in facultative...

  18. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ...awareness of FOP. Please consider running with us or making a donation via the link below. Please continue to...being with the company for about six months, I learned there was a Genzyme-based running team called Running...Second, Addgene will be hosting the AJ’s Boston League team (Contributing many new heroes to the team ...years. Additional Resources on the Addgene Blog Learn about Addgene's Previous Work with Rare Disease ...

  19. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    ...engineering from ZFNs through TALENs and CRISPR-Cas nucleases, provided important context for the rest of the...reagents so freely with the academic community has clearly made a huge impact on this field. Advances in ...described a chimeric Cas9 that is improving the nuclease specificity dramatically and reducing off-target... service questions for nearly 7 years, and my colleagues and I have an internal motto for scientists who..., and I found myself extremely grateful to my colleague Jason for choosing Simon Singh's "The Code Book...

  20. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    ...ever like to write about a recent plasmid deposit please sign up here.   Listen this episode of Hot Plasmids...contain a smaller RNA tag, are less prone to photobleaching, and achieve better signal in vivo as compared...increasing the brightness. Arne Fabritius and colleagues started with the FP mNeptune684 (originally described... rapid quantification of base-editing and Cas9 cleavage activity Article contributed by Angela Kaczmarczyk...show that the ACE reporter can also quantify Cas9 cleavage, thus exhibiting its versatile utility in quantifying...
Showing: 381 - 400 of 1019 results