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  1. MXS Chaining

    Type
    Blog Post
    ...expression) and a polyA signal (to terminate transcription) and the four constructs were then combined ...their 6-mer sequences, are rarely found in the transcriptomes of these species and can often be used to clone...

  2. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...HCab antibodies from its serum, and meticulously screening, optimizing, and characterizing several anti-mouse...review. If you just want to use the nanobodies described in Pleiner et al, the key steps for generating...

  3. DNA Purification Without a Kit

    Type
    Blog Post
    ...plasmid purification and DNA gel extraction method described in Kim and Morrison is nearly identical to that...Additionally, while commercial plasmid prep kits come in discreet sizes (mini, midi, maxi, giga) which requires ...

  5. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...standards (clear and detailed methods and image descriptions, a minimum n = 2, and well labeled high- and...’ results is available not through published manuscripts, but through word of mouth or old lab notebooks...

  7. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...enrichment (SELEX), some 1010 oligonucleotides could be screened for their affinity to a wide range of non-nucleotide... as the aptamer is bound to a protein fluor. Transcribing and translating a protein fluorophore also takes...

  8. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...the results of studies on reducing antibiotic prescriptions for respiratory tract infections. The video ...provide more than enough time for researchers to describe their science, and for viewers to decide if reading...

  9. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...calendar invite over to the r/Science team with a description of our AMA and Joanne’s preferred time to start...receive via email. The cool features are too many to describe here, but I have over 2,000 notes which Evernote...

  10. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...editing analysis Once the analysis is complete, a new screen appears with a graphical representation of the ...Data button on the bottom right of the analysis screen. ICE analysis details Contributions   The Contributions...

  12. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ..., and a PhD is not always necessary. The job description and the candidate requirements (and maybe also...customers, internal resources and databases are scrutinized, and this information is enriched with your technical...

  13. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...two pieces: a single protein, computationally described as a fusion of four Cas7 proteins and a putative... of landing sites via Cas9-directed reverse transcriptases, followed bylanding site recognition and integration...

  14. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...VSV-G is also toxic at high concentrations as described above and is inactivated by the complement system...proteins that can be used for specific purposes as described previously (Table 1, Gutierrez-Guerrero, et al...

  15. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...CRISPR-associated transposases” or MUCICAT. Here, we’ll describe each of these CRISPR systems. The original pCas...chromosomal integration of cargo without using screening markers. Capability to construct a strain library...

  16. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...synthesis companies, such as IDT, Genewiz and GenScript, offer tools to help with these decisions.  So...ATUM DNA2.0  IDT Synthetic gene design made easy GenScript- GenSmart Codon Optimization References Athey...

  17. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...s lab performed a series of positive selection screens in bacteria to identify mutants of S. pyogenes ...sequences (Kleinstiver et al. 2015). From these screens, they identified four novel SpCas9 variants with...

  19. Viral Production at Addgene

    Type
    Blog Post
    ...protocols, titering methods, and quality control are described below. AAV Production AAV distributed by Addgene...) are generated from lentiviral preparations (described above) that are subject to a concentration step...

  20. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...replicate—but not always to the same extent. As described in our previous Origin of Replication post, DNA...act as a feedback mechanism to reduce further transcription. Replication is also regulated by the balance...
Showing: 381 - 400 of 776 results