We narrowed to 570 results for: SHA

1. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

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   Blog Post

   ...nickases improve homology directed repair rates, and share their design rules for your next CRISPR nickase ...

2. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

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   Blog Post

   ...science and supporting scientists globally are the shared mission of both Seeding Labs and Addgene. The Addgene-Seeding...

3. Viral Vectors 101: Parts of the AAV Packaging Plasmid

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   Blog Post

   ...and endonuclease domains, all four Rep proteins share a common helicase and ATPase domains. To function...

4. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

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   Blog Post

   ...Bryan Roth lab for feedback on this article and sharing their expertise with us. References Urban DJ, Roth...

5. An Addgene Summer Internship

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   Blog Post

   ..., during the final week at Addgene. This report shared my work by breaking down the process of each day...

6. Antibodies 101: Fc Effector Functions

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   Blog Post

   ... to an antibody, can act as immune modulators, shaping the immune response by driving particular cells...

7. Tips for Improving Your Next Manuscript

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   Blog Post

   ... We use writing to communicate our findings and share them with the world. If one doesn’t write and publish...

8. Running for Rare Disease, Running for FOP, Running for AJ

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   Blog Post

   ...three years ago I decided it was time to get in shape. After three kids, I had put on typical middle age...

9. Management for Scientists: Managing vs. Leading

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   Blog Post

   ...Successful People Become Even More Successful, by Marshall Goldsmith, with Mark Reiter Harvard Business...

10. Advice for Choosing a Research Project

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    Blog Post

    ...before in your field?” He points out to “not be shackled by your own subject - maybe something used in ...

11. The Stingy Scientist: How the Baby Gel Box Was Born

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    Blog Post

    ...Andy Baltus. Addgene founder, Melina Fan, recently shared our protocol for making competent cells to save...

12. Choosing a Good Mentor for Scientists

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    Blog Post

    ...have good observations and be less inhibited in sharing concerns. Ask alumni of the lab. Most labs have...

13. History of CRISPR Cas - A tale of survival and evolution

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    Blog Post

    ...All organisms share an innate goal to survive. This past year, scientists hijacked survival tactics of...

14. Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life

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    Blog Post

    ...development. Today, ABRC does more than create and share resources, we seek out opportunities to engage people...

15. CRISPR Challenges: Standardization and Homology Directed Repair

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    Blog Post

    ...efficiency of insertion and gene replacement. Care to share your favorite CRISPR tool or resource? Let us know...

16. The Golden Stopwatch Award: Recognizing Facilitators of Research

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    Blog Post

    ...periodically for possible new awardees, and we’re happy to share stats and strategies with tech transfer offices....

17. Single-cell tracking of lineage and identity with CellTag

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    Blog Post

    ...reprogramming outcomes. For example, cells with shared CellTagD13 tags often had similar gene expression...

18. Antibodies 101: Epitope Availability

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    Blog Post

    ...well, don’t relax just yet. Proteins often change shapes and epitope accessibility naturally, by binding...

19. New Viral Vectors - Winter 2025

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    Blog Post

    ...serotype pAAV-syn-Flpo AAVrg Recombinase Ian Wickersham New viral prep pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40...

20. Antibodies 101: Chimeric Antibodies

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    Blog Post

    ...primary antibodies and the targets will be indistinguishable (top panel). If one of the antibodies is converted...