We narrowed to 665 results for: SIM;

...chalk talk? On the surface, a chalk talk is quite simple. It's you, a dry erase board, some markers (chalk...chalk talk Perfect your story and delivery. Design simple figures and diagrams to illustrate your vision....

...maintaining a stable concentration of plasmid. Similar to replication in E. coli, regulation primarily...replication of the plasmid proceeds (and terminates) similarly to the bacterial chromosome. Since replication...

...in mammalian cells. SaCas9 produced indels at a similar efficiency to SpCas9, leading the group to focus...two proteins, mediates protein trans-splicing, similar to an intron in pre-mRNA splicing. In 2016, Fine...

...executed on Linux, we created our first process by simply copying and pasting pieces from our existing script...existing script into the section of the next process. Similar to our first attempt, we manually inspected the...

...different RT enzymes and performed large edits with similar or better efficiency than PEmax, the current state-of-the-art...also make CAR-T cell therapy more accessible by simplifying the production process and could even enable ...

...experimental purposes. But what now? MoClo really simplifies vector construction by combining restriction ...software includes Golden Gate cloning tools that simulate the restriction–ligation reaction. You can input...

...from the cell. Steps 1-1 and 1-2 can be done simultaneously. Image from Li et al., 2021.   The updates.... The optimized plasmid curing method was much simpler and took only ~32 hours instead of ~60 hours. ...

...so great. If we all came from similar backgrounds, we would have similar cognitive biases and this would...

...to two fold for the final titer value, which is simply due to the noise of the assay. In other words, ...). However, because purified AAV preps contain similar and low levels of background protein and chemical...

... in different locations all over the world. But simply attending the conference is usually not enough....young professionals will never attend a job fair! Similar to a conference, job fairs bring you face-to-face...

...infections, we are treating microbes with compounds similar to those they have been battling for centuries.... that will be more difficult to treat later. A similar situation can occur if you treat a bacterial infection...

...healthcare consulting firms can be involved in many similar activities depending on the expertise of the senior... to a community of colleagues who are pursuing similar jobs and non-academic careers. Now, more than ever...

...work in cell culture. The problems with cell lines Simply put, the majority of cell line problems come from...monitor any changes. Your tracking system could be as simple as a piece of paper on the front of the incubator...

... or co-expressed with avidin or a structurally similar protein that was discovered in the 1960s, strepavidin... can label a protein of interest with biotin by simply creating a fusion in which AviTag is added to either...

...Reproducibility Project: Cancer Biology have shown similarly disappointing rates of reproducibility. These ...approach the situation differently, some adopting simple note-taking tools, others using excel sheets or...

...Scientists use deep mutational scanning to simultaneously test how multiple amino acid changes affect...functional screen of your liking. This method is similar to site-directed mutagenesis (SDM) PCR, but with...

... enough for all applications. To resolve this, similar systems have been developed for tracking various...animals only permits visualization of two colors. Similarly, Flybow adapts Brainbow 2.0 for use in Drosophila...

...cell spent passing through the laser beam. A is simply the area under the resulting curve. By default,...for both markers. When comparing two markers simultaneously, we commonly describe the resulting populations...

...this step as well. Preservation and Imaging Similar to Sample Preparation, this step can involve multiple...steps used in a non-fluorescent IHC/ICC assay are similar to the ones you plan to use, then the primary antibody...

...later, an A→G base editor (ABE) followed based on similar principles (Gaudelli et al., 2017). These editors...published on improving base editing precision. Similar treatments may one day have a smoother path to ...