...in mammalian cells. SaCas9 produced indels at a similar efficiency to SpCas9, leading the group to focus...two proteins, mediates protein trans-splicing, similar to an intron in pre-mRNA splicing. In 2016, Fine...

...executed on Linux, we created our first process by simply copying and pasting pieces from our existing script...existing script into the section of the next process. Similar to our first attempt, we manually inspected the...

...from the cell. Steps 1-1 and 1-2 can be done simultaneously. Image from Li et al., 2021.   The updates.... The optimized plasmid curing method was much simpler and took only ~32 hours instead of ~60 hours. ...

...different RT enzymes and performed large edits with similar or better efficiency than PEmax, the current state-of-the-art...also make CAR-T cell therapy more accessible by simplifying the production process and could even enable ...

...so great. If we all came from similar backgrounds, we would have similar cognitive biases and this would...

...amenable to multiplexing; expressing 5 gRNAs simultaneously produces comparable gene knockdown to single...LwaCas13a to shRNA, they found that LwaCas13a displays similar knockdown efficiency but no significant off-target...

...work in cell culture. The problems with cell lines Simply put, the majority of cell line problems come from...monitor any changes. Your tracking system could be as simple as a piece of paper on the front of the incubator...

...clones of the original cell. The plasmid DNA is similarly referred to as cloned DNA, and this process of... vary broadly in their functionality. In their simplest form, plasmids require a bacterial origin of replication... DNA fragments. Cloning vectors tend to be very simple, often containing only a bacterial resistance gene...so they can be distinguished from one another. Similar to DNA replication, the Sanger sequencing reaction...

...need to know the exact quantity of your POI, but simply whether its concentration changes in response to...controls are equally strong, so the normalized value simply reports which POI band is stronger. The right gel...

...Scientists use deep mutational scanning to simultaneously test how multiple amino acid changes affect...functional screen of your liking. This method is similar to site-directed mutagenesis (SDM) PCR, but with...

...cloning of your gRNA of interest. Figure 1: Simplified schematic of agrobacterium-mediated transformation...Shan Q, Zhang Y, Liu J, Gao C, Qiu J-L (2014) Simultaneous editing of three homoeoalleles in hexaploid ...

...Reproducibility Project: Cancer Biology have shown similarly disappointing rates of reproducibility. These ...approach the situation differently, some adopting simple note-taking tools, others using excel sheets or...

... enough for all applications. To resolve this, similar systems have been developed for tracking various...animals only permits visualization of two colors. Similarly, Flybow adapts Brainbow 2.0 for use in Drosophila...

... or co-expressed with avidin or a structurally similar protein that was discovered in the 1960s, strepavidin... can label a protein of interest with biotin by simply creating a fusion in which AviTag is added to either...

...cell spent passing through the laser beam. A is simply the area under the resulting curve. By default,...for both markers. When comparing two markers simultaneously, we commonly describe the resulting populations...

...this step as well. Preservation and Imaging Similar to Sample Preparation, this step can involve multiple...steps used in a non-fluorescent IHC/ICC assay are similar to the ones you plan to use, then the primary antibody...

... and note how they build suspense. Notice the simplicity of their slides and how the slides complement...cause and effect. Stories can and should be very simple. The goal is to get the listeners to be thinking...

...program to design primers for Gibson Assembly. For a simple example of using Gibson assembly, imagine that ...size, and purify the DNA fragments. Finally, you simply incubate these three fragments together along with...

...transcript constituted 59% of total dystrophin mRNA, similar to the 39% observed by Tabebordbar et al. Long ...therapy, it’s important to realize that DMD is a simpler case than other genetic diseases we’d like to treat...

...consumables (such as pipet tips, glassware, etc), get a simple list from your current P.I., lab manager, or administrator...need.  Prioritizing resources in your budget The simple practice of putting together a resource budget ...