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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 661 results for: SIM;

Showing: 381 - 400 of 661 results

  1. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...amenable to multiplexing; expressing 5 gRNAs simultaneously produces comparable gene knockdown to single...LwaCas13a to shRNA, they found that LwaCas13a displays similar knockdown efficiency but no significant off-target...

  2. How to Write a Scientific Review Article

    Type
    Blog Post
    ...Eventually I learned that it’s practical because there simply isn’t enough time to read every single paper. I...present it all in one place to get a sense of the similarities and differences among proteins in the class....

  3. Bioinformatics at Addgene

    Type
    Blog Post
    ...executed on Linux, we created our first process by simply copying and pasting pieces from our existing script...existing script into the section of the next process. Similar to our first attempt, we manually inspected the...

  6. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...maintaining a stable concentration of plasmid. Similar to replication in E. coli, regulation primarily...replication of the plasmid proceeds (and terminates) similarly to the bacterial chromosome. Since replication...

  8. The Challenges of Cell Culture

    Type
    Blog Post
    ...work in cell culture. The problems with cell lines Simply put, the majority of cell line problems come from...monitor any changes. Your tracking system could be as simple as a piece of paper on the front of the incubator...

  9. Hot Plasmids - November 2023

    Type
    Blog Post
    ...different RT enzymes and performed large edits with similar or better efficiency than PEmax, the current state-of-the-art...also make CAR-T cell therapy more accessible by simplifying the production process and could even enable ...

  10. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...infections, we are treating microbes with compounds similar to those they have been battling for centuries.... that will be more difficult to treat later. A similar situation can occur if you treat a bacterial infection...

  13. Plasmids 101: Biotinylation

    Type
    Blog Post
    ... or co-expressed with avidin or a structurally similar protein that was discovered in the 1960s, strepavidin... can label a protein of interest with biotin by simply creating a fusion in which AviTag is added to either...

  15. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...this step as well. Preservation and Imaging Similar to Sample Preparation, this step can involve multiple...steps used in a non-fluorescent IHC/ICC assay are similar to the ones you plan to use, then the primary antibody...

  16. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ... by a ribonuclease called Dicer. In a great simplification of the process, Dicer chops off the loop of...t always cleave the mRNA — the bound miRISC can simply block translation machinery. The composition of...

  18. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...cloning of your gRNA of interest. Figure 1: Simplified schematic of agrobacterium-mediated transformation...Shan Q, Zhang Y, Liu J, Gao C, Qiu J-L (2014) Simultaneous editing of three homoeoalleles in hexaploid ...

  19. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ... and note how they build suspense. Notice the simplicity of their slides and how the slides complement...cause and effect. Stories can and should be very simple. The goal is to get the listeners to be thinking...
Showing: 381 - 400 of 661 results