We narrowed to 718 results for: STO
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TypeBlog Post...biomarkers. The lab validated their platform on clinical stool samples by comparison to qPCR and demonstrated that...the SAP-RFP fusion. Then, the cells are washed to stop the production of new particles. With subsequent...
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Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog Post...coordinate emotions, thoughts, and behavior. Historically, scientists study the electrical activity in...interactions in cells because the protein binders can be customized for protein visualization, relocation, degradation... -
The time and cost required to make a plasmid
TypeBlog Post...closing in on the one million plasmids shared milestone in 2018, we wrote a post on the time and cost ...any of the numbers in this post is a mathematical story well beyond my ability to tell. But even from these... -
Plasmids 101: The protein expression toolbox
TypeBlog Post...for your protein of interest. CRISPR-Cas9 and a custom gRNA is all you need to generate a frameshift mutation... fused to a dead Cas9. The system is led, via a custom gRNA, to your gene of interest to temporarily increase... -
Summer SciComm Series: Cognitive Load
TypeBlog Post...refers to the work put into creating a permanent store of knowledge (a schema).” In scicomm, I like to.... It’s a lot easier to read a formulaic fiction story that doesn’t require any detailed retention of information... -
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards
TypeBlog Post...neurons whose function and connectivity are poorly understood,” says Qiu. Qiu developed a dual virus system... light activation and CNO silencing. (D) Immunohistochemistry verification of the dual virus expression... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog Post...directly visualized with fluorescence or immunohistochemistry methods, these proteins are routinely fused... also be identified using antibodies and immunohistochemistry techniques. Thus, these proteins provide... -
Developing Lab Management Software for Biology
TypeBlog Post...little simplified): Find the appropriate glycerol stock within our freezers Pick the sample into the appropriate...intentionally trivial, but they're here to help tell this story. In this first example to the right (# this will... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog Post...induces recombination at LoxP sites. Placing a lox-STOP-lox cassette upstream of a gene of interest blocks...Cre is absent. When Cre is present, it removes the STOP cassette, activating gene expression. Although Cre... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog Post...drug resistance, Bhang says “the [drug] resistance story panned out quickly,” and was summarized in a recent...would have different labels - i.e. they would stochastically arise from different cells throughout the treatment... -
Engaging with science and society at pgEd
TypeBlog Post...sticky ethical dilemmas to dark episodes in the history of eugenics and research misconduct. Past ethical... talk about science in a language that can be understood by those who are not experts in particular scientific... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post...cells survive their own secretions. Pro tip! Historically, colicin-producing plasmids have been referred... Sunagawa, S., Mclellan, S. L., Willis, A. D., Comstock, L. E., & Eren, A. M. (2024). A cryptic plasmid... -
A Guide to Designing a Scientific Poster: Content, Layout, and More
TypeBlog Post...beautiful figures. And, you can’t wait to tell your story. But, before you can show off your work at a conference...be the focus of your poster - the data and your story. If you choose a single-color background but want... -
Viral Vectors 101: Chemogenetics
TypeBlog Post...Resources References Chen X, Choo H, Huang X-P, Yang X, Stone O, Roth BL, Jin J (2015) The First Structure–Activity... https://doi.org/10.1523/JNEUROSCI.3480-15.2016 Weston M, Kaserer T, Wu A, Mouravlev A, Carpenter JC, ... -
Hot Plasmids - October 2020
TypeBlog Post...2020.07.02.185249 Predicting cell cycle length with a histone-fused fluorescent timer By Kate Harten DeMaio Studies...color-changing fluorescent timer (FT) protein to the histone H2B that allows for observation in live cells.... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog Post...reaffirming.” Now, she’s an Associate Scientist at Bristol Myers Squibb and also writes for the Addgene blog...you’re set up for failure. Listening to Kenkel’s story has helped me realize that is not true at all. Racheal... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog Post...enzymatic fusions are godsends. Tethering dCas9 to the histone acetyltransferase p300 or the DNA methyltransferase...disease has now become much like the 1980s song “Don’t Stop Believin’”; whenever you hear it (whether at a scientific... -
A Look at Addgene's QC Process
TypeBlog Post...Eventually, these errors could cause the plasmid to stop functioning properly or be something completely ...collection. This standardization provides security for requestors, ensuring that the plasmid they ordered contains... -
CRISPR in the Clinic
TypeBlog Post...Additional ongoing trials The CRISPR therapies don’t stop with the list above! There are many, many more in...Plasmids and Resources CRISPR 101 eBook CRISPR History and Development Resources on the Addgene blog... -
Plasmids 101: Terminators and PolyA signals
TypeBlog Post... we'll provide an overview on how transcription stops, or termination. Read on to learn more! Download...basic processes for eukaryotic termination are understood and it has been noted that each eukaryotic RNA...