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  3. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...integrate a reporter gene that contains a puromycin resistance gene followed by the target site (the same sequence...:  A donor plasmid that contains a puromycin resistance gene linked to an in-frame H2B-GFP. This visual...

  6. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...population is specific for your target antibody and may assist in gating your target population (b). There..., so you can use most of your samples for flow analysis rather than for setting up your initial voltages...

  7. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...The ITRs base pair with each other to allow DNA synthesis of the complementary strand to yield dsDNA. Unlike...ones listed previously). E3/E1B suppresses both apoptosis and the host immune response to the virus to allow...

  8. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ... day of the interview, dress the part, and be consistent with your personal statement, while you make ...success. Every student should use these guidelines consistently across a number of years as you apply for internships...

  10. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...emphasizing the importance of critical thinking, persistence, teamwork, and communication. And as a program...breathalyzer to pesticide breakdown, production of fire-resistant housing insulation, and cleaning up salt runoff...

  11. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...journal. An increasing number of funding bodies insist that the research data resulting from their funding...and make up their own minds on whether the data analysis has been of good quality and whether they agree...

  12. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...other genetic disorders, pre-implantation genetic diagnosis (PGD) screens embryos to find those that do not...determine its genotype. However, this detailed analysis is not possible with IVF, as most of the cells...

  13. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...antibiotic in the plate correspond to the antibiotic resistance marker present in your plasmid. You should also...is due to a PCR mistake or a mistake by the DNA analysis software. Now that you've read our plasmid troubleshooting...

  16. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...systematically and logically is as useful for finishing your thesis as it is for helping your team reach an important...thinking, decision making, creativity, or data analysis. Conveniently, these are all skills you are already...

  17. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...the inter-molecular annealing or second strand synthesis. In contrast, a scAAV vector with half the size...promote efficient transduction independently of DNA synthesis." Gene therapy 8.16 (2001). PubMed PMID: 11509958...

  18. All in a Twist: dsRNA

    Type
    Blog Post
    ...centromeres. These genomic regions are prone to persistent R-loop formation, presumably due to their abundance...considerations when using these reagents. RNA:DNA hybrids Persistent RNA:DNA hybrids that arise during transcription...

  19. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...using words for their field or technology in a consistent way. Just creating a lexicon was not enough. ... using the same words with the same meanings consistently, meetings will be shorter and more productive...

  20. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...technical details of the problem you're solving, but consistency is key. Some namespace collisions you may encounter...treating these pieces of data in ways that are inconsistent with how they are being treated in the lab, ...
Showing: 381 - 400 of 729 results