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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 738 results for: Sis;

Showing: 381 - 400 of 738 results

  1. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ... prepared me for what a PI without a personal assistant has to deal with. My inbox currently stands at...advisor Wally Gilbert: “Very interdisciplinary, with emphasis on genetic engineering, sequencing, and translation...

  2. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...have become a gold standard in gene expression analysis to investigate the effect of regulatory elements...you choose. Many luciferase assays require cell lysis as the most efficient means to disrupt the cell ...

  4. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...population is specific for your target antibody and may assist in gating your target population (b). There..., so you can use most of your samples for flow analysis rather than for setting up your initial voltages...

  5. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...The ITRs base pair with each other to allow DNA synthesis of the complementary strand to yield dsDNA. Unlike...ones listed previously). E3/E1B suppresses both apoptosis and the host immune response to the virus to allow...

  6. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ... day of the interview, dress the part, and be consistent with your personal statement, while you make ...success. Every student should use these guidelines consistently across a number of years as you apply for internships...

  7. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...emphasizing the importance of critical thinking, persistence, teamwork, and communication. And as a program...breathalyzer to pesticide breakdown, production of fire-resistant housing insulation, and cleaning up salt runoff...

  11. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...Less than 20 ng of total gDNA is sufficient for analysis. We have routinely successfully analyzed gDNA .... 6. Fu, Y. et al. High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells...

  12. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...Institute were instrumental in helping us establish consistent, high-quality protocols for lentiviral production...at Addgene. Our Board helps keep our focus on assisting scientists and accelerating research and discovery...

  13. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...integrate a reporter gene that contains a puromycin resistance gene followed by the target site (the same sequence...:  A donor plasmid that contains a puromycin resistance gene linked to an in-frame H2B-GFP. This visual...

  18. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...journal. An increasing number of funding bodies insist that the research data resulting from their funding...and make up their own minds on whether the data analysis has been of good quality and whether they agree...

  19. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...the inter-molecular annealing or second strand synthesis. In contrast, a scAAV vector with half the size...promote efficient transduction independently of DNA synthesis." Gene therapy 8.16 (2001). PubMed PMID: 11509958...

  20. All in a Twist: dsRNA

    Type
    Blog Post
    ...centromeres. These genomic regions are prone to persistent R-loop formation, presumably due to their abundance...considerations when using these reagents. RNA:DNA hybrids Persistent RNA:DNA hybrids that arise during transcription...
Showing: 381 - 400 of 738 results