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We narrowed to 794 results for: Tec

Showing: 381 - 400 of 794 results
  1. In Vivo Biotinylation of Bacterial Fusion Proteins

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    Blog Post
    ...Additional Posts on Mol Bio Techniques Find Additional Posts on Plasmid Technologies Resources at Addgene.org...Plasmids in the Repository Learn about New Plasmid Technologies in our Hot Plasmid Articles ...
  2. 10 Great Guest Posts We're Thankful For

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    Blog Post
    ...how they function. Adapting Toehold Switches to Detect Zika Virus The RNA-based toehold switches described.... These toehold switches can be used to quickly detect the presence of Zika virus. Read this post from...Combinatorial Genetic Screening CombiGEM is a technology that can be used to quickly barcode a set of ...
  3. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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    Blog Post
    ...present in prokaryotes, and some of these proteins protect against invading DNA. In 2014, Swarts et al. characterized...the Natronobacterium gregoryi Argonaute.” Nat Biotechnol. Epub 2016 May 2. PubMed PMID: 27136078. Find...Resources on Addgene.org Check Out Other Argonaute Technologies from the Doudna Lab Browse the CRISPR Collection...
  4. Addgene's Top 10 Blog Posts of 2015

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    Blog Post
    ...many developments in science and the plasmids, technologies, and researchers behind them. As part of our...started using plasmids and the revolutionary CRISPR technology in their own labs. We’re overjoyed to provide...will be sure to keep our eyes open for any new technologies that will bring about the next revolution in...
  5. Don’t Wait To Launch Your Health Science Startup

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    Blog Post
    ...individuals in their 20’s who’ve built multibillion dollar tech companies using only laptops and some server space...our network.  We started with the idea of a biotechnology investment fund that would apply a percentage... biotechnology...
  6. Another Pathway into Cells: iTOP

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    Blog Post
    ...type; however, their usage has been limited by technical problems. For instance, it’s much more difficult...proteins and reporters Previous Protein Delivery Techniques: Introduction of macromolecules into cultured...homing an artifact? Lundberg M, Johansson M. Nat Biotechnol. 2001 Aug;19(8):713-4. PubMed.  ...
  7. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

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    Blog Post
    ...Escherichia coli or Saccharomyces cerevisiae with few techniques that are easily transferrable across host species...for rational, straightforward design. Since the technique relies on base pairing, it has promise to be host-independent...engineering, this is such a powerful and flexible technique that it should be on the radar of every molecular...
  8. New and Upcoming Viral Vectors - June 2019

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    Blog Post
    ...AAVs for serotype testing jGCaMP7c for calcium detection PSAM4 GlyR for chemogenetic inhibition Flp expression...) AAV-hSyn-EGFP Biosensor AAV The ability to detect the neurotransmitters and other bioactive molecules...sensor. This “contrast” variant provides improved detection for tracking large populations of neurons in wide-field...
  9. Trading Spaces - Visiting Scientist Edition

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    Blog Post
    ...institute. A common reason is hands-on training in a technique that only a few labs have expertise in. Visiting...sure you read any relevant literature for the techniques you will be performing to familiarize yourself...help you with your research and teach you new techniques. Show up on time (don’t make your bench mentor...
  10. DAP arrays for multiplex gene editing

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    Blog Post
    ... for gene editing. They named this new array architecture the DAP array, short for “drive-and-process....payloads. Different from existing multiplex array architectures, including previous tRNA-gRNA arrays, that rely...Comparison of previous representative dual-gRNA architectures with DAP array   Qichen Yuan is a PhD...
  11. Synthetic Photobiology: Optogenetics for E. coli

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    Blog Post
    ...turns 10 years old, it’s easy to forget that this technique isn’t limited to neuroscience. In fact, precise... method for the computational problem of edge detection. Tabor et al. subsequently engineered a second...publication at Addgene. A synthetic genetic edge detection program. Tabor JJ, Salis HM, Simpson ZB, Chevalier...
  12. Finding and Joining Your Dream Lab

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    Blog Post
    ... and open source software, hardware, wetware, technology development, etc). Second, the potential hire...job satisfaction (especially for scientists and technical people) is linked to engagement and access to ...you would like to head for a career in pharma or biotech, this is one case where the topic of your research...
  13. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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    Blog Post
    ...acids in the domain). Yan et al. from Arbor Biotechnologies found that RspCas13d accessory protein RspWYL1...functional in multiple species (read Arbor Biotechnologies' press release about their Cas13d deposit here...Addgene. Learn more about research at Arbor Biotechnologies. Additional Resources on the Addgene Blog:...
  14. A Practical Approach to Choosing the B(right)est Fluorescent Protein

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    Blog Post
    ...fluorescent molecule. A higher brightness increases the detected signal and therefore high brightness is an important...brightness that does not fold well or cannot be detected in a microscopy setup has zero practical brightness...excitation wavelength, available emission filters, and detector sensitivity) and the biological system (temperature...
  15. Multiplex Genome Editing with CRISPR-Cpf1

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    Blog Post
    ...Reversing the orientation of the direct repeat protects the (+) stranded lentivirus RNA from Cpf1-mediated... is one of the latest developments in CRISPR technologies.  It’s a simple and effective method with multiple...CRISPR–Cpf1 Asing a Single crRNA Array." Nature Biotechnology 35.1 (2016): 31-34. Pubmed PMID: 27918548. 2...
  16. Behind the scenes: Addgene’s new search engine and more

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    Blog Post
    ...visible part behind these changes: the backend technology that powers the new website search. Tackling ... resources, blog posts or reagent items.  The technology behind Addgene’s new search  Like millions of...PI labs and institutions. There are many more technical details that make our new search engine unique...
  17. GPCRs: How Do They Work and How Do We Study Them?

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    Blog Post
    ...heterotrimeric G protein complex that can be used to detect G protein activation via bioluminescence resonance...response in vivo (Paek et al., 2017). In this technique, the receptor fused to APEX2 catalyzes the biotinylation...are then identified with mass spec. Using this technique termed “GPCR-APEX” the Kruse lab was able track...
  18. Viral Vectors 101: Types of viruses

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    Blog Post
    ...features with gamma-retrovirus including genome architecture, host integration, and its association with ...Schambach A. Gammaretroviral vectors: biology, technology and application. Viruses. 2011 Jun;3(6):677-713...An Overview of the Application of Viruses to Biotechnology. Viruses. 2021 Oct 14;13(10):2073. DOI: 10.3390...
  19. How Addgene Creates Content

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    Blog Post
    ...gaps and add to them as techniques evolve. Blog posts get out of date as techniques and knowledge changes... useful information on scientific literature, technical skills, and experiences from thousands of laboratories...
Showing: 381 - 400 of 794 results