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  1. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ... Rep68 to initiate replication and a terminal resolution site that serves as the target site for Rep proteins...structure. The Rep binding element (RBE) and terminal resolution site (trs) are noted. Created with biorender....

  2. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ... tap water. Flood the slide with Gram's iodine solution. Rinse briefly with tap water. Add ethyl alcohol...preparing the smear, and mixing the stains and solutions used, check out these resources.   Bruckner, ...

  3. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...enabling everyone to participate in the biotech revolution. Our lab exists in what appears to be a funky...proof-of-concept experiments for discovering novel therapeutics. Dave is a chemical biologist with a Ph.D. from...

  6. Celebrate National Book Month with Addgene

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    Blog Post
    ..., why it affects our emotions, and even what therapeutic properties it has. As with other works of Dr....about the science of race."     - Joanne Kamens, Executive Director  I Contain Multitudes by Ed Yong "Microbes...

  9. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...skills to apply those concepts and understand how therapeutic strategies work. Find Addgene Blog posts about...they had brilliant ideas on how to solve many therapeutic and pre-clinical problems! Who knows, perhaps...

  10. Visualizing Translation at the Single Molecule Level

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    Blog Post
    ... untranslated mRNAs. The high specificity and resolution of this system are achieved by using multiple...analysis in vivo of translation with nucleotide resolution using ribosome profiling.” Science 324(5924) ...

  13. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...more effectively used in a technology or as a therapeutic.  A (very) brief history GCE came onto the synthetic...to include reagents accessible to your lab. The solution GCE provides almost endless possibilities for ...

  14. Viral Vectors 101: Types of viruses

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    Blog Post
    ...gamma-retrovirus in the lab? Well, they are essentially evolutionarily optimized gene delivery devices. Their survival...naturally occurring and more synthetic capsids are routinely being developed) to choose from, which can target...

  17. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ... break site (Figure 2A). The single molecule resolution of digital PCR allows classification of individual...total gDNA is sufficient for analysis. We have routinely successfully analyzed gDNA from a few thousand...

  18. Behind the scenes: Addgene’s new search engine and more

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    Blog Post
    ...protocols for educational resources, and labs and institutions for depositors (see below). Even if the user...suggestions provide shortcuts to matching PI labs and institutions. There are many more technical details that ...

  19. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...we wanted to take a look back at some of the contributions from our depositors. Below are the 15 top requested... Their long-lasting popularity illustrates the utility these tools have in many different areas of biology...
Showing: 381 - 400 of 737 results