We narrowed to 750 results for: Uty

1. Celebrate Open Data Day with Addgene’s AAV Data Hub!

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   ...is driven by the dedicated community of data-contributing researchers who use AAV tools. This word...opportunity to cite the image to give proper attribution.  We hope that this provides a clear overview...

2. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

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   ...variety of journals. Since then, I developed a routine that works well for me to evaluate the submitted...general, I think that this is an (e.g. great contribution to the community because of..." This first couple...

3. Single-cell tracking of lineage and identity with CellTag

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   ...assay in the mouse intestine, demonstrating its utility in long-term tracking in vivo (Guo et al., 2019... induced pluripotent stem cells or the clonal evolution of cancer. If you’re looking to use CellTag, you...

4. Don’t Wait To Launch Your Health Science Startup

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   ...quite honestly, does not value your potential contribution to the healthcare landscape.  You will find ...partnerships with universities, companies, hospital institutions and funding bodies. Don't shy away from the ...

5. "Build Your Plasmid": The Game - Play to Teach

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   ...skills to apply those concepts and understand how therapeutic strategies work. Find Addgene Blog posts about...they had brilliant ideas on how to solve many therapeutic and pre-clinical problems! Who knows, perhaps...

6. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

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   ...your DNA: Concentration (ug/ml) = A260 reading x dilution factor x 50 ug/ml This method is quick and simple...You then run samples of your DNA at different dilutions and quantify based on band intensities of your...

7. Visualizing Translation at the Single Molecule Level

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   ... untranslated mRNAs. The high specificity and resolution of this system are achieved by using multiple...analysis in vivo of translation with nucleotide resolution using ribosome profiling.” Science 324(5924) ...

8. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

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   ...played enormous roles in enabling researchers to utilize and develop these tools by making their own fluorescent...Mek2, Cdk5, and Cas9. Xin has demonstrated the utility of these FLiPs in a variety of research applications...

9. Plasmids 101: Gram Negative and Positive Bacteria

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   ... tap water. Flood the slide with Gram's iodine solution. Rinse briefly with tap water. Add ethyl alcohol...preparing the smear, and mixing the stains and solutions used, check out these resources.   Bruckner, ...

10. DAP arrays for multiplex gene editing

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    ...for a higher number of MPE. Therapeutic potential  To explore the therapeutic potential of the DAP array...

11. Cultivating Community Science at BosLab

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    ...enabling everyone to participate in the biotech revolution. Our lab exists in what appears to be a funky...proof-of-concept experiments for discovering novel therapeutics. Dave is a chemical biologist with a Ph.D. from...

12. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

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    ... T helper type 2 (Th2) cells from mice that constitutively express Cas9 and screen for genes that affect...commonly called bad cholesterol). However, size distribution and concentration of ApoB-LPs has been shown...

13. Celebrate National Book Month with Addgene

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    ..., why it affects our emotions, and even what therapeutic properties it has. As with other works of Dr....about the science of race."     - Joanne Kamens, Executive Director  I Contain Multitudes by Ed Yong "Microbes...

14. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

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    ... a combination of computer modeling, directed evolution, site-saturation mutagenesis, and medium-throughput... green. A second colonizer, labelled with a constitutively fluorescent reporter can also be used to track...

15. R Bodies: Membrane-Rupturing Microscopic Tools

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    ...we can also learn a lot from Nature’s simpler solutions to transmitting forces over long distances. For...

16. How to Lead a Great Meeting

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    ...excited about the prospect of working toward a solution. At the end of the meeting, make sure to thank...Addgene Blog Get Management Advice from Addgene Executive Director Joanne Kamens FInd Tips on Picking a ...

17. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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    ...genetic manipulations. You can produce some very beautiful immunofluorescent images like in this paper (2...experimental assay that works with flies, you can utilize the resources of the Drosophila community to easily...

18. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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    ...each genotype with vulnerabilities) to design a therapeutic strategy that works. But, those darn cancers ... using attributes of cancer variants such as evolutionary conservation, or predicted deleteriousness of...

19. Building and Validating Recombinant Antibodies: A Case Study

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    ... the mAb heavy and light chain, each from a constitutively active CMV promoter. Constructs were then verified...plasmids have been deposited with Addgene for distribution to the scientific community with the plasmid...

20. Viral Vectors 101: Inverted Terminal Repeats

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    ... Rep68 to initiate replication and a terminal resolution site that serves as the target site for Rep proteins...structure. The Rep binding element (RBE) and terminal resolution site (trs) are noted. Created with biorender....