...pragmatic adoption (although it might be argued various authors have simply chosen to submit elsewhere)...effort. Our standards of data recording are highly variable and usually left to individual researchers. Yet... said to be representative or the statistical variation is calculated and placed in the legend. Widely...

... used by scientists today come in many sizes and vary broadly in their functionality. In their simplest...become an essential tool in molecular biology for a variety of reasons, including that they are: Easy to work...- Plasmids can drive gene expression in a wide variety of organisms, including plants, worms, mice, and... coding gene function, they are now used for a variety of studies used to investigate promoters, small...produced. Expression vectors can drive expression in various cell types (mammalian, yeast, bacterial, etc.),...is a group of newly synthesized DNA strands of varying lengths whose last nucleotide is labeled. Once ...

...have successfully helped researchers develop a variety of therapies. For example, many scientists use ...and easy to work with as they can survive in a variety of environmental conditions, and double every 2... Various Addgenies...

...fluorescent protein (GFP) from Aequorea victoria and its variants are genetically encoded fluorescent probes. One...photostability, maturation time, and acid sensitivity of various fluorescent proteins (Botman et al, 2018; Cranfill...linker length, the specific fluorescent protein variant, and where to add the fluorescent tag. These choices...

...constantly evolving and demanding field, requiring a variety of biological and molecular tools. One such tool... Arabidopsis include understanding of allelic variation between different plant species and metabolic ... Various Addgenies...

...common housekeeping proteins are influenced by a variety of experimental conditions (Ghosh et al., 2014)...that expression of your reference protein does not vary under your experimental conditions. Otherwise, you...control won’t be strongly influenced by unexpected variations in any individual protein. This ensures you are...

...splenocytes, you might only be interested in one of the various cell populations; for example, CD8+ T (“T killer...membrane-impermeant. With each cell division, the (invariant) amount of dye is reduced per cell (Figure 4a)...Research Associate in the lab of Arlene Sharpe at Harvard Medical School. His projects include investigating...

...processed piece of tissue, where cells are present that vary in cell type, size, and marker proteins. But even...parameters the machine uses to differentiate between variations in those factors. To understand the principles...Research Associate in the lab of Arlene Sharpe at Harvard Medical School. His projects include investigating...

...Krissy Lyon, a PhD candidate in Neuroscience at Harvard University. Just as computers, cell phones, and...accurately. We're sure to see many improvements in a variety of biological technologies as both time and research...Krissy Lyon is a PhD candidate in Neuroscience at Harvard University currently studying the serotonergic ...

...ll want to find in candidates’ resumes or CVs; “vivarium”, “weaning”, “colony”, etc. This will help save..., salaries in Kansas versus salaries in NYC will vary widely. Therefore, start up awards should reflect...respond by saying, “The exact start up costs may vary greatly. However, I have a list of what I really...

...Uses Viral Integration Serotypes Pseudotyping AAV Variants References AAV Components The small (4.8 kb) ssDNA...therapeutic applications. Clinical trials using AAV for various gene delivery applications are currently underway...of AAV-DJ, but with enhanced brain uptake. AAV Variants As mentioned above, AAV improvements have included...hybrid viruses with new properties. Other system variants include: Self-complementary AAV (scAAV): One downside...Mol Ther. 2009 Aug;11(4):442-447. PubMed . AAV variants: Self-complementary recombinant adeno-associated...

...Tet-Off vectors (Tet-Advanced)” are the most recent variants of the Tet-Off system.  For more information on...cells, this system has also been implemented in a variety of other organisms, such as sphingomonads and bacillus...pADH1 activity. Researchers have engineered pADH1 variants that are less sensitive to ethanol, including ...

... mutants, identifying the two  best-performing variants as iGluSnFR4f (fast deactivation) and iGluSnFR4s...new tools promise to be a popular choice for a variety of synaptic imaging applications. Find iGluSnFR4...nicking, producing STITCHR. STITCHR can make a wide variety of genomic edits — single-base changes, small deletions...

...instead activates/represses gene expression by various mechanisms. The RNA guide is typically 20 nts and...of optimization which have led to more specific variants. HypaCas9, sSpCas9(1.1), SpCas9HF1, and evoCas9...Every experiment is unique and off-target risks will vary depending on many factors. Hopefully now you have...

...polysaccharide structures, many of which are easily varied by the cell and whose structures are just beginning...recombinant proteins. Optimal salt concentration will vary with each protein, but several of the phage RBPs...Cloning Sytem to Clone Your Gene of Interest Into a Variety of Expression Vectors Deposit Your Microbiology...

...blogger Nathaniel Roquet, a PhD student in the Harvard Biophysics program and researcher in the Lu Lab...interference between the transcribed gene and its 5’ UTR. Various self-cleaving hammerhead ribozyme parts are described...Roquet! Nathaniel Roquet is a PhD student in the Harvard Biophysics program and researcher in the Lu Lab...

... in the cell line of interest. Importantly, by varying these ratios, you can either maximize or minimize...is a powerful technology that may be used for a variety of research applications, including making transgenic...over 6 years in the lab developing and using a variety of gene editing technologies including Zinc Fingers...

...While these benefits make AAVs useful for a wide variety of experiments, care still needs to be taken when...types. In the CNS, it has been shown that there is variable transduction of oligodendrocytes, microglia, astrocytes...Many AAVs will show significant expression in a variety of cell types, but you should try to find the best...

...integrations per cell, and my stable cell pool had wildly variable Cas9 expression (Figure 1). This is a problem ...monoclonal lines (Figure 2) and I was surprised at how variable Cas9-expression was across the different cells...expressing A549 cells transduced with BRAF gRNA at various MOIs. The presence of the 428 bp Surveyor nuclease...

...researchers feel that they lack formal training in a variety of transferable skills. At Addgene we've set out...spent my senior year isolating pheromones from various C. elegans mutants by myself, I would have slowly...team should have a leader, other team roles will vary depending on the type of team. Knowing what roles...