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We narrowed to 772 results for: Vars;

Showing: 381 - 400 of 772 results

  1. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...fluorescent protein (GFP) from Aequorea victoria and its variants are genetically encoded fluorescent probes. One...photostability, maturation time, and acid sensitivity of various fluorescent proteins (Botman et al, 2018; Cranfill...linker length, the specific fluorescent protein variant, and where to add the fluorescent tag. These choices...

  2. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...beat in terms of size. Like CRISPR, there is a variety of small RNA types to choose from and can be catered...translation machinery. The composition of the RISC varies, but at the very minimum requires an Argonaute ...19px;font-family: helvetica;","color":"#FFFFFF","variant":null,"font":"helvetica","style":"font-family: ...

  3. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ...common housekeeping proteins are influenced by a variety of experimental conditions (Ghosh et al., 2014)...that expression of your reference protein does not vary under your experimental conditions. Otherwise, you...control won’t be strongly influenced by unexpected variations in any individual protein. This ensures you are...

  4. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...splenocytes, you might only be interested in one of the various cell populations; for example, CD8+ T (“T killer...membrane-impermeant. With each cell division, the (invariant) amount of dye is reduced per cell (Figure 4a)...Research Associate in the lab of Arlene Sharpe at Harvard Medical School. His projects include investigating...

  5. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...processed piece of tissue, where cells are present that vary in cell type, size, and marker proteins. But even...parameters the machine uses to differentiate between variations in those factors. To understand the principles...Research Associate in the lab of Arlene Sharpe at Harvard Medical School. His projects include investigating...

  6. Evolution of Lab Techniques

    Type
    Blog Post
    ...Krissy Lyon, a PhD candidate in Neuroscience at Harvard University. Just as computers, cell phones, and...accurately. We're sure to see many improvements in a variety of biological technologies as both time and research...Krissy Lyon is a PhD candidate in Neuroscience at Harvard University currently studying the serotonergic ...

  7. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...ll want to find in candidates’ resumes or CVs; “vivarium”, “weaning”, “colony”, etc. This will help save..., salaries in Kansas versus salaries in NYC will vary widely. Therefore, start up awards should reflect...respond by saying, “The exact start up costs may vary greatly. However, I have a list of what I really...

  8. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ... mutants, identifying the two  best-performing variants as iGluSnFR4f (fast deactivation) and iGluSnFR4s...new tools promise to be a popular choice for a variety of synaptic imaging applications. Find iGluSnFR4...nicking, producing STITCHR. STITCHR can make a wide variety of genomic edits — single-base changes, small deletions...

  9. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...instead activates/represses gene expression by various mechanisms. The RNA guide is typically 20 nts and...of optimization which have led to more specific variants. HypaCas9, sSpCas9(1.1), SpCas9HF1, and evoCas9...Every experiment is unique and off-target risks will vary depending on many factors. Hopefully now you have...

  10. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...Tet-Off vectors (Tet-Advanced)” are the most recent variants of the Tet-Off system.  For more information on...cells, this system has also been implemented in a variety of other organisms, such as sphingomonads and bacillus...pADH1 activity. Researchers have engineered pADH1 variants that are less sensitive to ethanol, including ...

  11. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...polysaccharide structures, many of which are easily varied by the cell and whose structures are just beginning...recombinant proteins. Optimal salt concentration will vary with each protein, but several of the phage RBPs...Cloning Sytem to Clone Your Gene of Interest Into a Variety of Expression Vectors Deposit Your Microbiology...

  12. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ...blogger Nathaniel Roquet, a PhD student in the Harvard Biophysics program and researcher in the Lu Lab...interference between the transcribed gene and its 5’ UTR. Various self-cleaving hammerhead ribozyme parts are described...Roquet! Nathaniel Roquet is a PhD student in the Harvard Biophysics program and researcher in the Lu Lab...

  13. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ... in the cell line of interest. Importantly, by varying these ratios, you can either maximize or minimize...is a powerful technology that may be used for a variety of research applications, including making transgenic...over 6 years in the lab developing and using a variety of gene editing technologies including Zinc Fingers...

  14. Antibodies 101: Validation

    Type
    Blog Post
    ...and specificity. However, there are countless variables that impact an antibody’s affinity and specificity...very similar proteins. Additionally, they require variable expression of your target across samples - it ...it is hard to distinguish between specific, invariable expression and non-specific, background signal, ...

  15. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...vector for this step may save time as it reduces variability that may be encountered with DIO/FLEx vectors...off-target expression. Since recombination rates vary between every lot of AAV vector, a range of dilutions...rare for a methods section to include all of the variables that need to be accounted for (i.e. serotype, ...

  16. Important Considerations When Using AAVs

    Type
    Blog Post
    ...While these benefits make AAVs useful for a wide variety of experiments, care still needs to be taken when...types. In the CNS, it has been shown that there is variable transduction of oligodendrocytes, microglia, astrocytes...Many AAVs will show significant expression in a variety of cell types, but you should try to find the best...

  17. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...integrations per cell, and my stable cell pool had wildly variable Cas9 expression (Figure 1). This is a problem ...monoclonal lines (Figure 2) and I was surprised at how variable Cas9-expression was across the different cells...expressing A549 cells transduced with BRAF gRNA at various MOIs. The presence of the 428 bp Surveyor nuclease...

  18. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...researchers feel that they lack formal training in a variety of transferable skills. At Addgene we've set out...spent my senior year isolating pheromones from various C. elegans mutants by myself, I would have slowly...team should have a leader, other team roles will vary depending on the type of team. Knowing what roles...

  19. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...approach are thought to be off-target effects and variable silencing efficiency. To address these shortcomings...screening method was limited in two substantial ways: variable silencing efficiency and labor-intensive processes...sufficient degree remain alive. This likely reduces variability in the RNAi output and trivializes downstream...

  20. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...The mycoplasmas enter the cell culture through various sources that are difficult to trace. These include..., PCR primers specific to the 16s RNA genes of various commonly infecting mycoplasmas are used. A broad...coughing in the lab. The human mouth contains a wide variety of mycoplasmas and these can enter the cell culture...
Showing: 381 - 400 of 772 results