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...around the Addgene office as we peruse the #DeckTheLab submissions. We asked all of the Addgenies to pick...MerryChristmas #happyholidays2018 #DeckTheLab @Addgene @joachimgoedhart @SAbiega @EikeMahlandt @Saskia_deMan... @EmoryChem lab office looking festive 🎄🌟🎁 @Addgene #DeckTheLab pic.twitter.com/bDRqAKrWHz — Dyer Lab...winners will receive a holiday care package from Addgene. We hope all of our entrants had fun decorating... agar art from Nika Heijmans stood out to the Addgenies. Happy holidays!🎄☃️ More #agarart from our ...If you’re feeling as chilly this winter as the Addgenies are in Massachusetts, have a look at the Dyer ...

...experiments, let us know (datahub@addgene.org) and we might just send you a new Addgene Data Hub t-shirt!      ... out the redesigned, revamped, and re-launched Addgene AAV Data Hub, the place for scientists to share...curated collection of community submissions for Addgene and CLOVER AAV experiments - and we've updated ...Submitting to the Data Hub If you've ever validated an Addgene or CLOVER AAV, you can (and dare we say, should...step-by-step submission guide or email datahub@addgene.org to learn more. A great submission includes: ...

...experiment!” but were unable to find the plasmid at Addgene? Well, we’re here to help with our Suggest a Plasmid...Campaign. How to suggest a plasmid The scientists at Addgene work hard to seek out plasmids to add to our collections... potential depositors. 2. Tweet at us! Tweet @Addgene with #suggestaplasmid, the name of the plasmid ...

...our hot plasmids segment on the Addgene Podcast. Subscribe to the Addgene blog to get all the latest hot...transposon vectors tested in this work are available at Addgene. Kim, et al. Stem Cell Reports. 2015. PubMed PMID...spines. The set of CyRFP constructs available at Addgene includes bacterial and mammalian mCyRFP1 or CyRFP1...lab has deposited all mScarlet constructs with Addgene. Bindels DS, et al. Nat. Methods. 2017. PubMed... and Cre recombination. Find PhoCl plasmids at Addgene. Zhang W, et al. Nature Methods. 2017. PubMed ...check out the growing list of popular plasmids in Addgene’s FP collection. Just last month, Robert Campbell...Plasmids for CRISPR coselection are available at Addgene, and the Doyon lab has also provided a detailed...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Additional Resources on the Addgene Blog Listen to Our Podcast Interview with Eye Researcher Connie Cepko...Science Videos from Tom McFadden Resources at Addgene.org Sign up to Receive Our Newsletter Check Out ...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Additional Resources on the Addgene Blog Listen to Our Podcast Interview with Eye Researcher Connie Cepko...Science Videos from Tom McFadden Resources at Addgene.org Sign up to Receive Our Newsletter Check Out ...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Additional Resources on the Addgene Blog Check out additional microbiology resources Stem cell models for...additional plant biology blog posts Resources at Addgene.org Sign up to Receive Our Newsletter Check Out ...

... Science Training Resources on Addgene.org Check Careers at Addgene ...training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting...small to mid-sized companies and organizations. Addgene as a nonprofit organization is very considerate...the community of biological scientists who use Addgene everyday. To get this and other projects like it... it going, someone (likely several people at Addgene) had to take the initiative to find a suitable partner...outside your comfort zone As part of my position at Addgene I am now involved in Strategic Partnership Management...collaborations is important? In my current job at Addgene I work with people from various organizations in...

...Additional Resources on the Addgene Blog Important Considerations When Using AAVs Addgene's AAV Quality Control...performed on all new viral vector preparations. Addgene has used this method to generally validate our ...   Figure 1: Electron micrograph of an Addgene AAV viral vector preparation after negative staining...    Which titer do people usually report? Addgene and other viral vector manufacturing facilities...compared.   Figure 2: Label that comes on Addgene’s AAV aliquots. Our titer values (circled in red...improvement over qPCR (Taylor et al., 2017). What do Addgene AAV titers mean for your experiments? Because (...AAV in vivo to determine the optimal dosage. At Addgene, we don’t reserve lots, but if you’re reordering...

...Addgene was built on the concept of accessibility: making plasmids more accessible to scientists around...the next twenty years. But learn we did! From Addgene's very beginning, we had to consider digital accessibility... accessibility on our website.  As members of Addgene’s content team, we — Jo, Brook, and Angela — most... in a poster compared to an online abstract or Addgene blog post.  Here, we are presenting this image ... with the rest of the workshop.  For Figure 1, Addgene chose this alt text: "The left panel is a profile...writing!   Resources Additional resources on the Addgene blog Five Steps to Writing Grant Proposals to Grad...

...achieve temporary repression of protein expression. Addgene has a dCas9 KRAB plasmid for transient knockouts...Want to transiently induce expression? Check out Addgene's Lentiviral dCas9 SAM vector and Piggybac dCas9...on/off promoters and modes of action Check out Addgene's Lentiviral Tet-on and AAVS1 targeted Tet-on transgene...degradation. Adapted from Nabet et al. Check out Addgene's Lentiviral dTAG cloning vector and SMASh degron...PubMed PMID: 29581585. Additional resources on the Addgene blog Plasmid 101: Inducible Promoters Plasmid...for my Experiment Additional resources on the Addgene website Promoter Guide CRISPR Guide Lentiviral...

..., and that means it's officially the start of Addgene's winter holiday celebrations! We're kicking it ...Joachim Goedhart  Created in collaboration with Addgene writers High Complexity Golden Gate Assembly with...big thank you to all who have contributed to the Addgene blog this year! Your contributions have helped ...guest authors, while others chose to work with Addgenie writers to help share their research, tools, and...

... your research Subscribe to the Addgene blog Resources on Addgene.org Learn about the Michael Davidson...Allele Biotechnology that now can be deposited at Addgene. What makes these fluorescent proteins unique and...scientific community, please deposit the plasmids at Addgene! References Ai, Hui-wang, et al. "Directed evolution...PMCID: PMC3811051. Additional resources on the Addgene blog Read our fluorescent protein blog posts Find...Davidson fluorescent protein collection Check out Addgene’s fluorescent protein plasmids and resources Find...

...have probably heard about Addgene’s viral service but did you know that Addgene also performs independent...101 eBook Additional resources on Addgene.org Learn about Addgene's viral service Learn about viral production...research? Since launching the viral service in 2016 Addgene’s research team has been actively investigating ...PMCID: PMC2957237. Additional resources of the Addgene blog Read our beginner's guide to viral vectors...

...options Resources on Addgene.org Check out Addgene's job openings Find eBooks from Addgene, including the ...development tips, take a look at the Addgene Science Career Guide eBook Addgene Science Career Guide eBook.   ...   Resources Resources on the Addgene blog Learn about career planning resources What Do I Do Now? ...

...Natronobacterium gregoryi, or NgAgo for short. Addgene has already distributed this plasmid all over the...supplied. Find Plasmids from the NgAgo Publication at Addgene Cas9 and NgAgo head to head NgAgo has a number ...27136078. Find plasmids from this publication at Addgene. 2. Swarts, Daan C., et al.  “DNA-guided DNA interference...PMC4697943. Find plasmids from this publication at Addgene. 3. Swarts, Daan C., et al. “Argonaute of the ...PMCID: PMC4446448. Additional Resources on the Addgene Blog Read About Cas9 Homolog Cpf1 Check Out High...Genome Engineering Posts Additional Resources on Addgene.org Check Out Other Argonaute Technologies from ...

...ready to request from Addgene so you can combine the sensitivity of HiBiT with Addgene's quality control, ...Collection that express similar constructs available at Addgene. Read the original paper for more detail on whether...disruptive tag. Find HiBiT-TP53 Fusion Vector at Addgene! Following extracellular RNA editors Tracking factors...flow via EVs. Find ADAR-HiBiT Fusion Vector at Addgene! Watching protein degradation in real time Proteins...measurements. Find CFTR-HiBiT Fusion Vector at Addgene! Real-time tracking of receptor internalization...Find HiBiT-EGFR and EGFR-HiBiT Fusion Vectors at Addgene! Measuring messengers: growth factors Growth factors...receptor complex. Find sHiBiT-KDR Fusion Vector at Addgene! HiBiT advances detection of endogenous proteins...

...plasmids from this paper at Addgene. Additional Resources on the Addgene Blog Check Out Our CRISPR Featured... PMC5127784. Find plasmids from this paper at Addgene. 2. East-Seletsky, Alexandra, et al. "Two distinct...PMID: 27669025. Find plasmids from this paper at Addgene.  3. Gootenberg, Jonathan S., et al. "Nucleic ... PMC5526198. Find plasmids from this paper at Addgene. 4. East-Seletsky, Alexandra, et al. "RNA Targeting...: 28475872.  Find plasmids from this paper at Addgene.  5. Abudayyeh, Omar O., et al. “RNA targeting...PMID: 28976959. Find plasmids from this paper at Addgene. 6. Shmakov, Sergey, et al. "Discovery and functional...Multiplex Genome Editing with Cpf1 Resources on Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs...

... deposited the plasmids used for this study at Addgene which include a C-terminal deletion in the spike...containing these mutations have been deposited at Addgene. Plasmids deposited from Alejandro Balazs’s lab... Bst polymerase constructs from their assay to Addgene.  Drew Endy’s and Philippa Marrack’s labs also ...investigate how the virus interacts with human cells. Addgene’s mammalian target plasmids express these and other...Brunello (knock out) and Calabrese (activation), from Addgene to better understand which human genes are important...10.1089/crispr.2020.0030 Additional resources on the Addgene blog Learn more about mapping the SARS-CoV-2 -... Plasmids & Resources page Stay up-to-date on Addgene's operations during the pandemic Find SARS-CoV-2...

...26411297. Resources at Addgene Find CRISPR plasmids at Addgene Read Addgene's CRISPR Resource Guide View...26422227. Find plasmids from this publication at Addgene. 2. Zetsche, Bernd, et al. "Multiplex Gene Editing...27918548. Find plasmids from this publication at Addgene. 3. Kim, Daesik, et al. Genome-wide analysis reveals...27272384 Find plasmids from this publication at Addgene. Kleinstiver, Benjamin P., et al. "Genome-wide...27347757. Find plasmids from this publication at Addgene. Makarova, Kira S., et al. "An updated evolutionary...