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Showing: 381 - 400 of 1091 results

  1. Introducing the Addgene Antibodies 101 eBook!

    Type
    Blog Post
    ...Addgene is very excited to share the very first edition of our Antibodies 101 eBook! The latest addition... always, don’t hesitate to reach out to help@addgene.org with any thoughts, questions, or feedback. Happy...

  2. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...posts @Addgene: The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years Addgene’s Top...2011, it was only recently made available through Addgene and we want to highlight it for use in your research...systems have been developed and deposited with Addgene which allow scientists to use CRISPR-Cas technology...determining the structures are now available from Addgene - bfloGFPa1 and bfloGFPc1. Bomati et al., Sci ...further reading about SpyLigase technology, read the Addgene interview with Mark Howarth. Shining a light...ChrimsonR, CsChR, and CoChR have been deposited to Addgene, including lentiviral and AAV expression vectors... of various optogenetics plasmid tools, visit Addgene’s optogenetics guide. More optogenetics tools...

  3. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...addition to useful plasmids and toolkits available at Addgene.  C. elegans open resources The C. elegans Neuronal...Kyoda et al., 2012).  C. elegans plasmid tools at Addgene In addition to the wealth of open resources described...highlight a few toolkits that we have available at Addgene that were discussed primarily at the workshops ...interest.  SapTrap Tool Kit for CRISPR Cas9 edits At Addgene, we have the entire SapTrap toolkit from the Jorgensen...resources about C. elegans and the plasmids we have at Addgene check out our worm expression resources page. This...PMCID: PMC4689222. Additional resources on the Addgene blog Learn more about SapTrap Read about CRISPR...Browse our Plasmids 101 blog posts Resources on Addgene.org Visit our worm plasmids and resources page Browse...

  4. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...maps. Resources on Addgene.org Sequence Analysis of your Addgene Plasmid Addgene Plasmid Guide     ...This post was updated on Dec 4, 2017. At Addgene, we continually use the Basic Local Alignment Search...databases, and elements of the BLAST search pages. At Addgene, we use blastn to identify any discrepancies in... viewing blastx results. When using blastx at Addgene, we use the default “Non-redundant protein sequences...NCBI BLAST website. Additional Resources on the Addgene Blog: Plasmids 101: How to Verify Your Plasmid...

  5. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...discuss the importance of actionability on the Addgene blog and how SITN short-form blog posts help readers...Additional Resources on the Addgene Blog Resume Writing for Non-academic Science Careers 10 Steps to a Perfect...

  6. CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats

    Type
    Blog Post
    ...was contributed by guest blogger, member of the Addgene Advisory Board, and Associate Director of the Genetic... that model. Both libraries are available from Addgene as pools provided in two sets, each with 4 gRNAs...individual plasmid formats A first for a library at Addgene, you can also order the individual lentiGuide gRNA...PubMed Central PMCID: PMC4744125. Resources on the Addgene Blog Learn to Conduct Genome-wide Screens with...CRISPR for Single Base Editing Resources on Addgene.org Browse Our New and Improved Pooled Library Page...

  7. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...the wide array of Cas9s (many available through Addgene’s repository) and make it easy to find your perfect...variety of species and cell types can be found on Addgene’s website. Activating target genes using dCas9-based...variety of species and cell types can be found on Addgene’s website. Fig. 3: Cas9 repressors include...What do i do next? Designing or selecting a sgRNA Addgene may carry a validated sgRNA for your target gene...Next CRISPR Experiment Cas9 expression systems Addgene carries a variety of Cas9 containing plasmids that... ). 368, 290–296 (2020). More resources on the Addgene blog Read all the posts in our CRISPR 101 Series...Library Experiments Additional resources on the Addgene website Find CRISPR Plasmids Catch up on your ...

  8. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...plasmids with Addgene. Additional control plasmids for the TKO library will be available at Addgene soon. Hart...cellular compartments (cytosol, Addgene catalog #71245; mitochondria, Addgene catalog #71247) Rosella provides...read? Listen to our hot plasmids segment on the Addgene Podcast. View Plasmids from:  March (Q1) | June...sequencing. The STARR-seq backbone is available from Addgene in both human and fly versions. Arnold, et al....alongside a growing number of CRISPR libraries from Addgene’s repository that can be used to perform screens...PMID: 24395366 Oxford Drosophila CRISPR library Addgene has had the privilege of distributing several human...promoter and Cas9 from the actin 5C promoter. Addgene’s fly library web page offers more details and protocols...

  9. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...Additional resources on the Addgene blog Read about company culture at Addgene See some of our previous ...costumes, and spooky science stories. Yes, please! At Addgene, we love finding ways to incorporate science themes...teams dressed up as plasmids, Blugene, and the Addgene ping pong table. This year, in addition to the ...Halloween is no exception. For the past 10+ years, Addgenies have formed teams, chosen a theme for the costume...

  10. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ...to highlight some of the top articles from the Addgene blog this year. Spoiler alert: many of the top ...SARS-CoV-2, including many with plasmids deposited at Addgene! 2. DNA Purification Without a Kit by Beth Kenkel...

  11. Addgene’s Top 10 Blog Posts from 2014

    Type
    Blog Post
    ...community, so email us at blog@addgene.org if you are interested. Addgene's Most Popular Posts of 2014 ...year in science – as seen through the lens of Addgene’s blog and plasmid repository. Our blog is just ...

  12. Find and Share AAV Data with Addgene's New AAV Data Hub

    Type
    Blog Post
    ...promoter in rat. Contribute your AAV data Just like Addgene’s plasmid repository, the AAV Data Hub is a community-driven...only accepting data for materials distributed by Addgene.  Future directions As the AAV Data Hub grows, ...accelerate your research. Sincerely, Melina & The Addgene Team ...

  13. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    ...plasmids you’d like to deposit with Addgene, be sure to visit addgene.org/deposit. Who knows, maybe yours ... articles cite the use of pMD2.G obtained from Addgene. Skimming the list, one gets a sense of the broad...searchable list here). Tips for using pMD2.G At Addgene, we use pMD2.G for producing our ready-to-use lentivirus...Lentiviral Transduction Additional Resources on Addgene.org Check out our Viral Service Learn more about...

  14. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    ...tools. Find the mouse CRISPR knockout library at Addgene. Henriksson et al., Cell, 2019. PubMed PMID: 30639098...cardiovascular disease. Find the LipoGlo plasmids at Addgene! Lawler et al, QJM: An International Journal of...systems. Find these CRISPR activation plasmids at Addgene. Liu et al., Nature Communications, 2019. PubMed...worms and have made these constructs available at Addgene. These include Arch(D95N) and QuasAr, as well as...biosensors at Addgene. Hashemi et al., PNAS. 2019, PubMed PMID 31371514. ... Various Addgenies...

  15. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...Additional Resources on the Addgene Blog Read Additional Cancer Blog Posts Addgene's Cancer Collection Pages... between tumor and immune cells in real time. Addgene depositor Professor Max Krummel from UCSF showed...engineering (Ref 5)  as described by Blue Flame Addgene depositor Professor Michel Sadelain from the Memorial...poster is pTomo-Ras which has been deposited at Addgene was used to produce the glioblastoma model for ...with the scientific community, contact deposit@addgene.org to find out more about our free deposit and distribution...: Deciphering the Relationship Resources on Addgene.org Browse Cancer Plasmids Browse Immunology Plasmids...

  16. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    ... planning an experiment to performing one.  At Addgene, over 120 labs have deposited CRISPR reagents, ...already been shown to work in CRISPR experiments. Addgene’s dedication to resource sharing has led us to develop...table columns are target gene, species, sequence, Addgene plasmid ID (if we have one associated with the ...Sharing. 2016. In press. Additional Resources on the Addgene Blog Learn How to Design Your gRNA Use the CRISPR...This post was written jointly by Addgenies Brook Pyhtila and Nicole Waxmonsky Resource sharing shortens...of Our CRISPR Posts Additional Resources on Addgene.org Browse gRNA Design Tools Read Our CRISPR Guide...

  17. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ...University Medical Center are now available at Addgene along with a description of their experimental ...The three plasmids which have been deposited to Addgene are: pAdSh.PGK.Cas9, pAdSh.U6.gRNAS1, and pAdSh.U6...Maddalo et al., 2014). These tools are available at Addgene and could be useful to labs wishing to create this...including protocols, check out the plasmids at Addgene: pAdSh.PGK.Cas9 (expresses S. pyogenes Cas9 from...technology guides, FAQs, and CRISPR resources on Addgene's CRISPR page. References Beatty MS, Curiel DT ....1089/hum.2015.087 Additional resources on the Addgene blog How to Design Your gRNA for CRISPR Genome...

  18. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...adeno-associated viral (AAV) vectors available at Addgene are suitable for in vivo studies, our scientists...measuring viral titer and assessing purity. But Addgene is also conducting more rigorous quality control...plexWell technology, we can now perform NGS at Addgene to sequence all the DNA packaged inside the viral...AAV-68544, the best match for the contaminant is Addgene plasmid #44362. Method validation, limitations,...commonly performed for research-grade materials. Addgene is going the extra mile and pioneering the systematic... Press, pp 247–278 Additional resources on the Addgene blog Check out our Viral Vectors Featured Topic...discuss. We are also available by phone or at help@addgene.org to answer your questions about our quality control...

  19. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    ...yielding promising results in the laboratory, and Addgene is accelerating this research by helping to make...possible. Connecting the research community - Addgene's cancer collection pages Scientists are making...latest resources? In response to this challenge, Addgene has launched an initiative to curate our collection...direct the investigator to a list of all available Addgene plasmids he or she can use to study it (as seen...section below. Michael G. Lemieux is a Scientist at Addgene with interests in education and scientific writing...Institute: Ras Pathway v2.0 Page Resources on the Addgene Blog Tackling Cancers' Drug Resistance with a ...Used in Cancer Lineage Tracing Resources on Addgene.org Visit our Cancer Collection Pages Deposit Your...
Showing: 381 - 400 of 1091 results