...retrofitting our car with a freezer wired to a charger and two deep cycle batteries for power so the samples... voltmeter on top of the battery box to monitor charge status and a disconnect switch between the battery...spring requiring an overnight hike in. Navigating challenges: collecting and processing environmental samples... Paul and Anastasia (Potanina) Neddersen characterized a few samples by 16S rRNA gene sequencing which...thermostable Cas9 enzymes have previously been characterized: GeoCas9 from the Doudna lab and ThermoCas9 ...

...researchers working on rare diseases, offering them the chance to win some of the latest tools and technologies...They now have some theories on the underlying mechanism, involving the primary cilia. They’ll put a variety...number of stressors, including emotional stress or changes in temperature.  “The disease was first described...a rare disease that can cause hearing loss and changes in coloring of the hair, skin, and eyes. The researchers...syndrome (WS4) by Pediatrician Duangrurdee Wattanasirichaigoon carried different mutations in a gene called...

...was researching, but I decided I needed a new challenge. Looking for sales and marketing jobs Like most... choices we make, my job choices were mostly by chance. Luckily, around the time I decided to look for...have been their target market for many years and purchased their products, so you have some of the best ideas...started with the Harvard Business Review or some Chartered Institute of Marketing reviews, I would have struggled...post-doc a Cambridge University where he studied mechanisms of cell division. Recently Seán got the entrepreneurial...

... to a channel number based on intensity. Higher intensity events are assigned to higher channels. In a...then flow cytometry can help you overcome these challenges and make these types of experiments easier and...of the protein. In some cases this can lead to changes or a complete loss of protein function. In addition...fluorophores Fluorescent antibodies get around the challenges of fluorescent protein fusions by binding to ...stream oscillates to generate droplets that are charged as they pass through a metal deflection plate. ...

...Fluorophore detection Live APC-Cy7 channel eGFP FITC channel CD45 BV421   Figure...of fluorophores from Figure 1. For example, the chart tells us that PE and PE-Cy7 are "very bright" when...conjugated to Cy7, however, (APC-Cy7) the brightness changes to "dim" for the same laser type. Knowing that ...fluorophore, we have to tell the cytometer which channel to use to detect eGFP. As you saw in Figure 4, ...overlaps with FITC, hence reading out the FITC channel in the cytometer's software (without using the ...

...above) include Channelrhodopsin2 (Chr2), a blue-light sensitive non-specific cation channel, activation ...inhibition of action potential generation. Archaerhodopsin (Arch) is activated at a similar spectrum as...reliably locate your injection site. We recently purchased this model ( no longer available through Kopf)...editorial board, Jacqueline N Crawley  [et al]. 2012;Chapter 1:Unit 1 20 1-18. doi: 10.1002/0471142301.ns0120s59...PMC3337962. 8. Zhao S, et al. Cell type-specific channelrhodopsin-2 transgenic mice for optogenetic dissection...

...research projects.   Understanding drug resistance mechanisms of Mycobacterium tuberculosis Dr. Patricia Sheen...her group are interested in understanding the mechanisms behind Mycobacterium tuberculosis resistance ... these proteins will be tested. Investigating changes in neuronal activity after ischemic brain injury... investigates the molecular and cellular etiopathomechanisms of ischemic stroke, the most common type ... Tsakanova to image cortical neurons and study changes in neural activity after ischemic brain injury....

...constructs with AAV. In the case of channelrhodopsins, fewer channel proteins are required for the transduced...is to increase the AAV concentration. In this flowchart, you can see the dwindling proportion of AAV particles...gets transported up the cell’s axon. While the mechanism for this function is not yet fully understood,...S9-S15. PubMed PMID: 11841606. 2. Schultz BR, Chamberlain JS. Recombinant Adeno-associated Virus Transduction...

...necessarily to chat, but to mimic your usual work environment.  Be kind to yourself. This is a challenging period...Receive and provide support while being safe. @GHP_HarvardChan — Shekhar Saxena (@ssaxenageneva) March 16,...give back by supporting local businesses (ex: purchasing a gift card to use later or ordering take-out...exploration site Inventing Heron has a radio reporter challenge, and code.org has coding tutorials for all ages...

...transcriptional changes. Using this road map, and what was known about previously characterized TFs, the Zhang...association of the uncharacterized TFs is a useful finding. Transcriptional program changes from this screen...can be used with other selection and screening mechanisms, although the unique barcodes for each TF help...

...and numeracy on perceived climate change risks. Nature Climate Change, 2(10), 732-735. doi:10.1038/nclimate1547...sources of information if those sources affirm or challenge their beliefs. I asked participants to read short...read an abstract for a study with findings that challenged their beliefs, they were much more likely to ...a phenomenon called “motivated reasoning,” the catchall term for a range of neat little tricks our minds...

... virus used. All of these expression control mechanisms require a specific modification of the viral ... years ago. Since then, medical technology has changed a lot, but viruses have stuck around. Vaccine development...Viral vectors are being explored as the delivery mechanism for increasingly popular nucleic acid-based vaccines...specifically AAV, are also being exploited as delivery mechanisms for gene therapy. The tractability of AAV, low...1742-4690-4-66. PMID: 17888184; PMCID: PMC2075523. Bulcha, J.T., et al. Viral vector platforms within the...

...open-source microscopy seeks to change this. This recent push gives users the chance to understand the "black...much more involved. Challenges such as the provision of an accessible supply chain for parts that are ...Krishnamurthy, Ethan Li, Pranav Vyas, Nibha Akireddy, Chew Chai, Manu Prakash, "Squid: Simplifying Quantitative ...

... the materials in our catalog are very well-characterized. During this time, we’ve also created a variety...this, throughout the year we rolled out multiple changes to our website, including the recent homepage and... a look at the least visible part behind these changes: the backend technology that powers the new website...granularly customize the search engine, and a scoping mechanism that allows category-specific, narrow searches...among others. We also created a normalization mechanism to “collapse” variations and synonyms of the same...

...designed by the Toettcher Lab, OptoNBs are photoswitchable engineered nanobodies whose binding of their...small (~15 kDa), single monomeric variable heavy chain antibody domain.  How do these components interact... Sons of Sevenless (SOS), a guanine nucleotide exchange factor that activates Ras. They expressed this...message reaches the nucleus where gene expression changes lead to cell proliferation and differentiation....Optogenetic control of protein binding using light-switchable nanobodies." BioRxiv (2019): 739201.  Additional...

...results. GBEs seemed to follow the same initial mechanism as in mammalian cells — by excising the freshly... A's, producing C → A edits. Figure 3: Mechanism of cytosine transversion editors. Red arrows indicate...agreed on the first part of the likely editing mechanism: cytidine deaminase converts C to U, and UNG excises... Base Editing terms   Base transition Base changes between purines (A ↔ G) or between pyrimidines ...pyrimidines (C ↔ T).   Base transversion  Base changes from purine (A or G) to pyrimidine (C or T) or vice-versa...Glycosylase base editors enable C-to-A and C-to-G base changes. Nature Biotechnology, 39(1), 35–40. https://doi.org...

...go ahead with raises. What will these further changes mean? The abrupt reversal in salary raises at institutions...Continuing efforts to gather information and expedite change We are continuing to update our FLSA and Postdocs..., under “How institutional plans have/have not changed since the injunction”. Postdocs around the country...enterprise and promote their voices in driving changes in science. Follow him on Twitter @BiophysicalFrog...the Addgene Blog Read Gary's Previous Article: Changing Labor Laws Bring Increased Postdoc Wages Read ...

... in D.C. for a few years, Susan was ready for a change of scenery. She moved to Seattle and continued ...to articles for the Fred Hutch website, social channels and Hutch magazine. Getting pulled in all of these...finding that uninterrupted time to be creative is challenging,” says Susan.  Another difficulty is that some... scientist or someone who’s affected by climate change or affected by a superbug. I think we solve these...you enjoyed this series, subscribe to the Career Channel of the blog so you don’t miss out on future career...

...from a camelid antibody lacking a light chain, called a heavy chain antibody (Muyldermans S., 2013). They...antibody: An ~150kD protein with both light and heavy chains made by immune systems. But here, we're not interested... or less immunogenic version is needed.  Single-chain fragment variables (scFvs) are ~27kD engineered ...They are comprised of the variable heavy and light chain regions, connected by a short linker peptide. The...

...matter of applying that framework to the myriad challenges we encounter. As you spend time in a lab learning...protocol modifications, and ultimately decided on the changes to implement. As with any skill, there will always...yourself if you missed any important details. Or challenge yourself to think of more creative solutions. ... know-how and more about how you approach new challenges.     Know your strengths! Research experience...employer that you are well equipped to work through challenges from day one in your new position. Of course,...