We narrowed to 645 results for: emb

...and equal protein transfer from the gel to the membrane. A loading control is critical for data interpretation.... Always probe the loading control on the same membrane that the target protein was measured on.    Flow...

...enhancers. Although the lab used this vector in chick embryos, it also functions in mammalian expression systems...vivo cis-regulatory interactions in the chicken embryo. BioRxiv: https://doi.org/10.1101/135525. Find...

...selection marker, then electroporate it into mouse embryonic stem cells. After selecting for clones with the...the mutation and inject the ES cells into a mouse embryo to create chimeric mice. This laborious process...

...Show suitability for an intended application - Remember, just because your antibody performs as expected...is just as helpful as knowing when it will. And remember, antibodies are like puppies. Just because an ...

...contributed by guest blogger, Addgene Advisory Board member, and Institute Scientist at the Broad Institute.... While this seems obvious, it is important to remember that the same is true when designing gRNAs for...

...the background. Dyes that can pass through cell membranes can prove useful for live-cell imaging. Experimental...coupled receptors. Biochimica et Biophysica Acta - Biomembranes, 1838(1), 15–33. https://doi.org/10.1016/j.bbamem...

...commonly added to aid in protein purification. Just remember to remove the stop codon for C-terminal tags and...yeast expression vector that adds a SV40 NLS Myr Membrane localization pWZL-Neo-Myr-Flag-DEST - ...

...Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors...PMC3161101. 20. Chang, C.J., et al., Production of embryonic and fetal-like red blood cells from human induced...

...imaging and other applications. And see PalmGRET , a membrane-anchored version useful for labeling extracellular...Addgene. Last Updated Content last reviewed on 26 September 2025. Catalog items are updated more frequently...

...ribonucleoprotein particles (RNPs) to both zebrafish embryos and the mouse inner ear. Fourth-generation cytosine...Tsang in August 2020 and by Emily P. Bentley in September 2025. References and Resources References Chen...

...read our three tips to improve HDR efficiency . Remember that HDR also requires donor DNA to serve as template...can transfect at high efficiency, such as human embryonic kidney 293 (HEK293) cells. For more difficult ...

...via genetically encoded proteins. PhoCl is a new member of this class that works via a different mechanism...ribonucleoprotein particles (RNPs) to both zebrafish embryos and the mouse inner ear. These exciting papers ...

...concentration, and the cell lines used previously. Remember that the orthogonal pairs of aaRS and tRNA that...window) Last Updated Content last reviewed on 19 November 2025. Catalog items are updated more frequently...

...answered.  Interested? You can learn more here - and remember, submissions are due Nov. 21!   ...

...tagged with #DeckTheLab throughout the month of December are eligible to win a: That will warm up the ...

...what we lacked were videos guiding completely new members of the lab or those taking introductory lab courses...

...tagged with #DeckTheLab throughout the month of December are eligible to win a hot chocolate party that...

...to Addgene, Aavery!       Original post: In December, we introduced you to Blugene’s new virus friend...

...repository in ways that will be most useful to you, the members of our community.  ...

...! Executive Director Chonnettia Jones and Board Member Joanna Brownstein were also on the welcoming committee...