We narrowed to 437 results for: ena

1. Plasmids 101: Broad Host Range Plasmids

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   ...spp., Alcaligenes spp., Aliivibrio salmonicida, Anabaena spp., Azospirillum braziliense, Azotobacter spp...

2. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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   ...genome modifications. Cas9 has been modified to enable researchers to knock out, knock in, base edit, ...

3. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

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   ...FLP-FRT, Cre-lox, and how combinations of FLP and Cre enable additional levels of genetic control. What is FLP...

4. 5 Reasons to Use Reddit for Science Communication

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   ...moderators for keeping the quality high and thus enabling a productive discourse. They even advised me on...

5. CRISPR Activation: A Practical Guide

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   ...Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H,...

6. Tips for CRISPR Gene Editing in Mice

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   Blog Post

   ...that could be used to study a wide variety of phenomena, it’s possible that we’ll learn much more from...

7. Getting the Most from Your Lentiviral Transduction

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   Blog Post

   ...Majdoul, S., Martin, S., Merten, O.W., Galy, A., Fenard, D. ”Influence of mildly acidic pH conditions on...

8. Tips for Using FRET in Your Experiments

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   Blog Post

   ...Indeed, FRET was one of the first techniques which enabled the measurement of single molecule interactions...

9. Fluorescent Proteins 101: When GFP lets you down

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   Blog Post

   ...module that can report on location. This approach enables the study of proteins in cells, but not other biomolecules...

10. The Fluorescent Vegetables in Aptamer Soup

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    ...PubMed Central  PMCID: PMC5811451. Dolgosheina, Elena V., et al. "RNA mango aptamer-fluorophore: a bright...

11. Plasmids 101: Biotinylation

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    ...determined to be due to a deficiency in Vitamin H later renamed Vitamin B7/biotin. Egg white injury would plague...

12. Five Popular Model Organisms, Part 2

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    .... (2018). Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria...

13. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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    ...which, by undergoing two rounds of gene-editing enables scarless introduction of an intended mutation while...

14. Transferable Skills Guide: Conflict Resolution

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    Blog Post

    ...nobody is really owning. Conflict in this latter scenario usually manifests as the assumption “I thought...

15. Antibodies 101: Single Chain Fragment Variables (scFvs)

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    ...intensity of the fluorescent signal is boosted and enables single molecule tracking in living cells. The HA...

16. New Optogenetic Tools for Cytoskeleton and Membrane Control

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    ...GTPase activity on a global to subcellular scale, enabling precise control over vascular endothelial barrier...

17. Plasmids 101: Expressing shRNAs for RNA Interference

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    ...target. RNAi is a valuable tool for your research arsenal, and there is still a dedicated field of study ...

18. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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    ...  Transfecting these three plasmids into cells enables the production of lentiviral vectors that are competent...

19. Antibodies 101: Beyond Surface Labeling

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    Blog Post

    ...while fluorescence-activated cell sorting (FACS) enables you to include further (non-lineage) markers alongside...

20. CRISPR 101: Homology Directed Repair

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    ... the repair of breaks that lack a second end to enable DSBR or SDSA.   Homology directed repair and genome...