Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 11 results for: ena

Showing: 1 - 11 of 11 results

  1. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...base pairs. The initial denaturation step is commonly performed at 94–98°C. Denature 30 seconds at 94°C: ...time and temperatures. Initial Denaturation for 2 minutes at 94°C. Denature for 30 seconds at 94°C. Anneal... of reagents and a basic heating and cooling (denaturing and annealing) cycle. The process became automated...withstand many heating and cooling cycles, which would denature DNA polymerases from other species. In addition...in an appropriate buffer, a series of heating (denaturing) and cooling (annealing) steps allow the Taq ...nanograms of template DNA through several cycles of denaturation, annealing, and synthesis. After the PCR is ...Chain Reaction (PCR). Basic PCR Program Initial Denaturation for 2 minutes at 94°C: This initiation step ...

  2. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...with your plasmid Resuspension buffer Denaturing solution Renaturing solution 2 mg/mL RNase A TE or water-saturated...plasmid DNA, into solution. Add a renaturing solution to the denatured bacteria. Note: This step brings...that is compatible with the next solution. Add a denaturing solution to the resuspended bacteria. Note: This...mM EDTA Store Solution I at 4°C Solution II - Denaturing Solution 0.2 N NaOH 1.0% SDS Store Solution II... II at room temperature Solution III - Renaturing Solution (Potassium Acetate) 120 mL 5M Potassium acetate...clear and thicker as the proteins and DNA are denatured. Pro-Tip Do not vortex at this stage or the genomic...

  3. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...generating cell lines using lentiviral vectors enables long-term protein expression studies. Moreover,...resistance (e.g., puromycin, blasticidin), which enables selection of a stable cell culture after transduction...Performing antibiotic selection on transduced cells enables elimination of untransduced cells, resulting in...

  4. AAV ddPCR Titration

    Type
    Protocol
    ...min) Ramp Rate (°C/sec) # Cycles Denaturation 95 10 2 1 Denaturation 95 0.5 2 50 Annealing/Extension 60...

  7. Gibson Assembly Protocol

    Type
    Protocol
    ... assembled antibiotic gene. This trick can also enable replacement of "inverse PCR" reactions with a two-part...

  8. Pipetting Protocol

    Type
    Protocol
    ...molecular biology. You’ll use a pipette in many scenarios to accurately dispense small amounts of liquid...

  10. AAV Production in HEK293 Cells

    Type
    Protocol
    ...cells. Pass the cells twice a week during the maintenance phase and do not allow cells to reach 100% confluence...

  11. Lentivirus ddPCR Titration

    Type
    Protocol
    ...°C/sec) # Cycles Enzyme Activation 95 10 2 1 Denaturation 94 0.5 2 40 Annealing/Extension 60 1 2 40 Enzyme...
Showing: 1 - 11 of 11 results