We narrowed to 636 results for: erf
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TypeBlog Post...also included a feature to help save you from performing any unnecessary cloning - the list of "Associated...
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How Exercise Purges Stress and Keeps Depression at Bay
TypeBlog Post...wondering, depression shows up in mice as poorer performance on forced swim tests and less interest in sweets... -
6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results
TypeBlog Post...sequencing If your sequencing facility requires you to perform your own Big Dye PCR amplification reaction (as... -
Keeping Up With CRISPR/Cas9
TypeBlog Post...The New York Times and Forbes respectively: "A Powerful New Way to Edit DNA" and "This Protein Could Change... -
Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors
TypeBlog Post...Organizing that event and seeing the outcome was wonderful! I also volunteer for a dog rescue organization... -
Science Communication for Everyone
TypeBlog Post...and consider their background. Social media is a powerful tool. Use hashtags effectively. This would help... -
Stepping into a Virtual Internship: My Experience at Addgene
TypeBlog Post... careers. I figured this internship would be a perfect way of exploring my different interests but also... -
Bringing Sustainable Practices to the Lab: Innovations
TypeBlog Post...better for the environment without sacrificing performance or quality. To do this, we can use the ACT label... -
Editor's Choice, September 2016
TypeBlog Post...also our best month ever! Hats off to all of our wonderful writers and all those who have helped edit over... -
Editor's Choice, August 2016
TypeBlog Post...Cas9 Activators: A Practical Guide 10 Steps to a Perfect Science Talk Developing Lab Management Software... -
Modulate the Activity of 17 Signaling Pathways with One Kit!
TypeBlog Post...published studies in the literature to curate the perfect library, obtained the appropriate cDNAs from other... -
From our Table to Yours: An Inside Look at Lunch at Addgene
TypeBlog Post...lunch. I think these random lunches would be a wonderful tradition for university departments to establish... -
SciComm with the Experts at Science in the News Part 2
TypeBlog Post...for Non-academic Science Careers 10 Steps to a Perfect Science Talk 5 Reasons to Use Reddit for Science... -
IDP and your PI: A Roadmap for Career Planning and Personal Development
TypeBlog Post...As DePace says, science is full of “terrible perfectionists,” and we don’t often give ourselves credit ... -
Tag Your Favorite Yeast Genes with Ease
TypeBlog Post...fluorescent tags in S. cerevisiae, looking at their performance in categories such as brightness, stability, ... -
Plan Your Experiment
TypeGuide...Activation/interference Needs good primer design Western blot Knockouts Activation/interference Needs a ... nterspaced S hort P alindromic R epeats) is a powerful system that enables researchers to manipulate ...Selecting the genetic manipulation you plan to perform will help you narrow down which components you’...optimization can be challenging Prime Edit Repress or Interfere (CRISPRi) Reduce gene expression without permanently...effective than dCas9 alone for mammalian cell lines Interfere Activate (CRISPRa) Increase gene expression without...be effective, as the Cas9 bound to the DNA can interfere with transcription elongation. CRISPR Activation...between on-target and off-target activity. In a perfect world, your gRNA sequence would be an exact complement... -
What's New in CRISPR - September 2019
TypeBlog Post...has previously used the library with SpdCas9 to perform pooled CRISPRi screens to identify essential genes... -
2023 and 2024: Reflections and Looking Ahead
TypeBlog Post...comes at the start of every new year, it is a wonderful opportunity to once again reflect on our achievements... -
New Norepinephrine Indicators: nLightG and nLightR
TypeBlog Post...sensitive than green fluorescent proteins. nLightR did perform similarly to GRABNE, and having both colors allows... -
Supporting Rare Disease Research with BeHeard 2017
TypeBlog Post...model to identify SMN1 interacting partners and perform whole animal screens for small molecules that block...