We narrowed to 825 results for: mos

...viruses that prey on bacteria) may be the most numerous and most diverse biological entities on our planet...recognition factors of phages) constitute one of the most popular classes of phage proteins to study. These...

... recognizing mentee Lucía Ramos (University of Valencia, Spain) as the most improved trajectory during...during the pilot, and Joaquín López (MIT, USA) as most outstanding mentor in this first program. Although ...

... used. Inducible lentiviral transfer vectors The most widely used inducible lentiviral vector system is...this, you can use the transfection reagent you are most comfortable with. If you don’t have any experience...depends on the type of cell you would like to infect. Most of the protocols you will find will recommend using...

...development, the history of optogenetics begins almost 50 years ago with the discovery of microbial rhodopsins...electrical signal down the axon (Lodish et al., 2000). Most neurons in the brain are not naturally light-sensitive...system due to their relatively stable extra-chromosomally long-term expression and low ability to induce...

...ensuring the PAMs remain on the chromosome after DSB formation, was most efficient (Farboud et al., 2019...Wieland I, Gaul U, Forstemann K (2014) Efficient chromosomal gene modification with CRISPR/cas9 and PCR-based...

... Specialist in Patrick Hsu's lab at UC Berkeley. Most of the scientists I’ve met began doing research ...epidemiology, public health, and more.     A few of the most common roles that you’ll see in a lab. Position...dedicate your time and energy to. How can I make the most of my experience in the lab? Ask your questions—...

... to correct only a minority of those variants in most cell types. But prime editing, a CRISPR technique...efficiency of HDR is relatively low, meaning that most Cas9-induced DSBs are repaired by non-homologous... end joining. As a result, Cas9 treatment causes most products to be indels while the efficiency of HDR...application, and the efficacy varies widely — it still almost always results in higher ratios of desired edits...binding site, increase processivity, or improve thermostability. What came out on top? The Cas9 nickase fused...

...the number of cells needed is lower, and perhaps most important, targeting all genes doesn’t necessarily...group works on immune cells in different contexts. Most of the immune cells we study are migratory, and ...candidate genes. Green Listed, as of now, contains most of the full genome knockout human and mouse libraries...

...where excitation and emission are the most efficient (i.e., happen most of the time). Figure 1 shows a graph...molecule), their bleed is so strong that these spectra almost overlap. Hence, they should not be used within ...

...features would allow for the best performance in the most contexts (Figure 1). Together the features with ... situation (Devaraj & Finn, 2021). In one of the most common Click Chemistry reactions, one component ... But the reactive groups of Click Chemistry are almost completely absent from living systems. What are...the ER and Golgi apparatus and outside the cell, most cysteines are oxidized in disulfide bonds. Reactions...

...take a quick flash back to 2004. “Shrek 2” was the most popular movie in theaters, the Razr was the hottest...request for a kit came on March 9, 2005. It took almost six years to reach our first major repository milestone...distributing ready-to-use viral vectors from some of our most popular lentiviral and AAV vectors, as well as developing...guys rock." — Andrew York, Calico Life Sciences. Most of all, we’re incredibly thankful for all the scientists...

...the CTD (also known as the PI), while REC includes most of the bridge helix. Created with BioRender.com....conformation frequently (Osuka et al., 2018), but it’s most often shown in an “open” state, with the NUC and...region is disordered, Cas9 binds DNA very weakly, most likely through unstructured electrostatic interactions...

...same chemical reaction performed by CBEs. However, most CBEs include an inhibitor of base excision repair... a C → G editing efficiency of up to 68% at the most favorable target sites. Differential cytosine editing...on to develop more efficient and precise editors. Most researchers agreed on the first part of the likely...

...Thermo Scientific NanoDrop is one of the most popular options, so most of this post is based on reading results...detect by spectrophotometry than others. The ones most likely to impact absorbance readings are phenol,... of RNase treatment to degrade bacterial RNAs in most purification protocols reduces the risk of RNA contamination...

...alternative approach is RNA interference (RNAi). The most common method is transfection of short interfering...protein), which has been designated CD365 in the most recent HLDA workshop (HLDA10), as an example. For...cell lines using these two antibodies. This is the most usual level of evidence supplied to support the ...

....  The information presented may not reflect the most current legal developments.  Further, this post ... to their areas of interest. Additionally, since most universities and non-profit organizations do not...applicable to scientists). High Salary –not relevant to most postdocs. Commercial successes in the performing...

...vectors in fast-growing bacteria first. In fact, most yeast vectors and many mammalian vectors are designed...will be lost from the population. A bacterial chromosome has a single origin of replication (ori), where... the cells that contain it. Antibiotic selection Most bacterial plasmids used in the lab contain an antibiotic...gene for growth in bacteria. Expression You’re almost there: you’ve planned for vector replication and...

...material is right for their experiment.     Table 1: Most popular guides, protocols, and collections of 2023...visitors in 218 countries. In Table 1, you can see our most popular educational resources for each type of resource...different countries (Figure 4). Four of the five most popular videos are protocol videos, where showing...a step can provide needed clarity (Table 1). Our most popular video, however, is “What is a Plasmid?”,...

...such as malaria and toxoplasmosis. Despite their importance to global health, most apicomplexan genes ...bidirectional promoter, integrate at 11 specific chromosomal locations, and stably express the integrated ...are derived from the Ralser lab's own pHLUM minichromosomal vector, and besides being used for restoring...

...regalada a Addgene por Daniel Mejías Sánchez, ¡y no podríamos estar más emocionados! Después de recibir la traducción...científica profesional, L. Denise Roche, para editarla. Estamos orgullosos de anunciar que CRISPR 101: Una herramienta...