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  2. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...antibodies, we have developed a criterion that includes accuracy, strength, and reproducibility. We test... negative control to a sample with the primary included to confirm that the signal is from the primary...

  3. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...sequences in DNA resulting in a variety of outcomes including excision or insertion, inversion, translocation...Lab constructs targeting GABA neurons. Serotypes include AAV1, and AAV9. Find viral preps here pAAV-hSyn-Cre-P2A-dTomato...

  5. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...order to see an effect. Hemizygous mice can be included in breeding strategies identical to those used...with transgenic mice that can impact reproduction include embryonic lethality, a short lifespan, or infertility...

  9. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...widespread use of FRET with fluorescent molecules, including fluorescent proteins, has led to the alternative...measuring the change in FRET efficiency, from which conclusions regarding the proximity of the two proteins can...

  10. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...plasmids use Rho-independent terminators, which include naturally occurring terminators, such as T7 and...mammalian terminators (SV40, hGH, BGH, and rbGlob) include the sequence motif AAUAAA which promotes both polyadenylation...

  11. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...quantification tend to be overestimates since they can include free p24 and defective viral particles. Direct ...infectious virus in a prep as the measurement will include defective particles. Studies have found that values...

  12. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...biologists working together on a variety of projects including the OpenWorm project. The main goal of OpenWorm... track them. We have a number of microscopes, including an automated microscope but we need to develop...

  13. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...website and also for offline use via GitHub (which includes prediction in batch mode). To predict the best...passion for computational biology. His expertise includes applying machine learning techniques to make sense...

  14. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ...compensation between each other. We can therefore conclude that this population is double-positive for both...that there is no issue with compensation here. Conclusion Compensating between your fluorescent markers...

  16. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...introduce yourself and what you do. You might want to include your university and your advisor’s name for some... uploading your work to a preprint server (and include this information on the poster) so your visitors...

  18. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...standard quality control assays for AAVs. These steps include measuring viral titer and assessing purity. But...also conducting more rigorous quality control, including next generation sequencing (NGS) of the viral ...
Showing: 381 - 400 of 758 results