We narrowed to 457 results for: nsf

1. Plasmids 101: Codon usage bias

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   ...Charles G. Kurland. "Growth rate dependence of transfer RNA abundance in Escherichia coli." The EMBO journal...

2. Designing Your Chalk Talk for the Academic Job Interview

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   ... for presenting a science talk Check out our transferable skills guide Read all of our career blog posts...

3. To Codon Optimize or Not: That is the Question

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   ...important? Translation and protein synthesis relies on transfer RNAs (tRNAs). tRNAs bind to and deliver amino ...

4. Hot Plasmids - August 2020

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   ...structures within cells. HeLa cells were transfected with plasmids encoding FPs tagged with the plasma...

5. Antibodies 101: Immunoprecipitation

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   ...protein of interest, such as a sample transiently transfected or stably infected with the protein of interest...

6. Bioinformatics at Addgene

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   ...automating the bioinformatics steps needed to transform the raw sequencing data into a complete plasmid...

7. New CRISPR Tools: Cas7-11 and PASTE

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   ...target. In addition, when they looked at the transformed E. coli expressing Cas7-11, they found no evidence...

8. Career Insights: Technical Support Specialist

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   ... The benefit is that these skills are highly transferable and will help you enter other roles in project...

9. Data Freedom: The Expansion of Data Sharing in Research Publications

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   ...responsible for storage.  If datasets are too large to transfer to a journal repository, they are likely too large...

10. Antibodies 101: Beyond Surface Labeling

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    ...downstream applications, like an in vivo adoptive transfer of those CD8+ T cells, require the cells to be...

11. Harnessing Bacterial Toxins for Allelic Exchange

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    ...rely on exogenous enzymes, require multiple transformation and plasmid curing steps, and can generate ...

12. Five Popular Model Organisms, Part 2

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    ...Arabidopsis. There are also important tips for plant transformations and the use of Arabidopsis in the classroom...

13. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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    ...retinal gene therapy Learn about the parts of an AAV transfer plasmid Resources on Addgene.org: Check out ...

14. CRISPR 101: Validating Your Genome Edit

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    ...optimizing this step, or consider enriching for transfected cells by flow sorting or drug selection, assuming...

15. Plasmids 101: Biotinylation

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    ...and decarboxylases. These enzymes catalyze the transfer of carboxyl groups to organic acids to produce...

16. Evolution of Lab Techniques

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    ...fragmented into smaller pieces, run on a gel, and transferred to a membrane for analysis through hybridization...

17. Thoughts on the Future of Research 2015 Boston Symposium

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    ...the training they receive as postdocs is not transferrable to other environments. Slide from Paula Stephan...

18. Hot Plasmids: Spring 2025

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    ...(LAMP5) — as well as cholinergic (choline acetyltransferase, ChAT) neurons. The authors characterized ...

19. CRISPR 101: Off-Target Effects

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    ...genome. While this may effectively serve as a transfection or transduction control, a different gRNA would...

20. A Control for All Seasons

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    ...equal loading across samples and equal protein transfer from the gel to the membrane. A loading control...