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We narrowed to 466 results for: organization Addgene

Showing: 381 - 400 of 466 results

  1. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...DNA and lentiviral/retroviral vectors. Used by Addgene for pooled library amplification. F' mcrA Δ(mcrBC-hsdRMS-mrr...of genetic markers for your reference. Browse Addgene's curated list of Bacterial Expression Systems. ...blog post covering E. coli and others are model organisms! Our Most Popular Plasmids 101 Posts: Origin...

  2. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...this year!  Find Addgene's own Lentiviral backbone plasmids here! Figure 2: Addgene cloned EGFP into...Lenti CMV N-HA Puro (Addgene #236079) to create pAG Lenti CMV HA-EGFP Puro (Addgene #236081), then generated...particles in HEK293T cells with pMD2.G (Addgene #12259) and psPAX2 (Addgene #12260). HeLa cells were infected...Anti-GFP [N86/38.1R] (Addgene #180084), 0.01 µg/mL Anti-HA [12CA5] - Chimeric (Addgene #198003)) and Goat...increased sensitivity and tailored deactivation rates Addgene's own empty lentiviral backbones New recombinant...2025.03.20.643984.  Build your way: Explore Addgene's new empty backbones By Meghan Rego You asked, ...EGFP-expressing lentiviral backbones designed right here at Addgene. Here's what makes these new backbones special:...

  3. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...of the Tet-Off system.  For more information on Addgene Tet plasmids, see our Tetracycline resource page...has also been implemented in a variety of other organisms, such as sphingomonads and bacillus.  ADH1 The...PMC2942869. Find plasmids from this publication at Addgene. del Valle Rodriguez, Alberto, Dominic Didiano...PMC2883883. Find plasmids from this publication at Addgene. Wei, Xing, Christopher J. Potter, Liqin Luo &...PMC3846601. Find plasmids from this publication at Addgene. Riabinina, et al. “Improved and expanded Q-system...PMC4344399. Find plasmids from this publication at Addgene. Riabinina O, Potter CJ. The Q-System: A Versatile...PubMed PMID: 22129153. Additional Resources on the Addgene Blog Quick guide to working with Drosophila Terminators...

  4. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...s toolbox. Depending on the growth rate of the organism of interest, from the day primers arrive, the ...PubMed PMID: 24733238. Additional resources on the Addgene blog Read our Plasmids 101 blog posts Find our...about Lambda Red recombineering Resources on Addgene.org Find plasmids for allelic exchange Read our ...

  5. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...functional, fluorescent protein in almost all organisms and cell types, (ii) no need to add a co-factor...PMCID: PMC5837189. Additional Resources on the Addgene Blog Read our Introduction to GFP Read our Fluorescent...mTurquoise2, not shown) remains fluorescent in acidic organelles (Figure 1), showing that its acid tolerance is...Fluorescent Proteins 101 eBook Resources on Addgene.org Browse the Fluorescent Protein Collection Find...

  6. Evolution of Lab Techniques

    Type
    Blog Post
    ... abundance. If a scientist wants to know if an organism has a particular gene mutation, southern blotting...PubMed Central PMCID: PMC4411503. Resources on the Addgene Blog Learn More about Restriction Cloning Get ...Check out Plasmid Cloning by PCR Resources on Addgene.org Check out Our Protocol Pages Find Molecular ...

  7. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...which allow AAVs to broadly spread throughout an organism, including difficult-to-target tissues like the...number to the capsid. Most vectors provided by Addgene are pseudotypes using the genomic structure of ...system. Table 2: Availability of AAV serotypes at Addgene. Serotype In-stock viral prep Packaged on Request...capsid is a good option for your experiment? Use Addgene’s AAV Data Hub to find experimental reports on commonly...10.1128/jvi.00895-06  Additional resources on the Addgene blog Viral Vectors 101: An Introduction to AAV...validation data after your experiment! Thanks to Addgenies Brian O’Neill and Ina Ersing for contributing ... Vectors 101: Systemic Capsids Resources on Addgene.org AAV Guide AAV Data Hub AAV Packaged On Request...

  8. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...by visiting Addgene's Optogenetics Guide . Browse optogenetics plasmids available at Addgene. CRISPR/Cas9...Vectors 101 Youtube Videos Addgene's Biosafety Resources Resources on Addgene's Blog Parts of the AAV Transfer...In-Stock AAV Preps Resources on addgene.org AAV Plasmids Available at Addgene Viral Vectors 101 eBook Viral...glossary at the end! Browse AAV plasmids available at Addgene. Adeno-Associated Virus versus Adenovirus Adenoviruses...information on cloning and working with plasmids, visit Addgene’s Molecular Biology Reference . Production The three...vivo models. For more detailed protocols, see Addgene’s Viral Vector Protocols . Figure 3: Overview of...genes. rAAV episomes can develop chromatin-like organization and persist in non-dividing cells for a period...

  9. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ... transposons are delivered to cells or a model organism, they insert into the host’s genome. Then, the....3390/ijms22105084 Additional resources on the Addgene blog Learn about the INTEGRATE system for bacterial...

  10. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...impact of CRISPR editing in the model cell line or organism. Several options exist for unbiased off-target...PubMed PMID: 27494557. Additional Resources on the Addgene Blog Learn to Use Cas9 Activators Perform Genome-Wide...Models with CRISPR/Cas9 Additional Resources on Addgene.org View Our CRISPR Guide Pages Find CRISPR Plasmids...

  11. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...Resources on Addgene.org Addgene's Antibody Guide Ready-to-use antibodies in the Addgene repository Antibody...different in nature (eGFP being a protein and FITC an organic molecule), their bleed is so strong that these ...sailing, and reading.   More resources on the Addgene blog Antibodies 101: Introduction to Gating in ...

  12. Modular Cloning Guide

    Type
    Guide
    ...cloning (MoClo) and find MoClo kits deposited with Addgene, including the MoClo Toolkit and the MoClo Plant...et al., 2011. MoClo Plasmid Kits Available from Addgene Browse or search the table below to find MoClo ...at 18 levels for rapid characterization in new organisms. Mobius Assembly Vector Toolkit Bacterial Expression...reconstituting the ubiquitination cascades of different organisms in E. coli . MetClo DNA Assembly Kit Bacterial...Platinum TALENs for use in a wide variety of target organisms. TALEN Kit Genome Engineering, TALEN Stephen Ekker...fusion proteins for use in many different model organisms. Diatom uLoop Assembly Kit Other, Yeast Expression...tags, and signal peptides, for use in a range of organisms including diatoms, yeast, plants and bacteria....

  13. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Alternatively, gRNA-only plasmids can be used in cells or organisms in which Cas9 has been integrated into the genome...Many gRNA empty vectors have been deposited at Addgene. To find the gRNA vector for your system, you can...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  14. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...fluorescent dyes have enabled the visualization of both organic tissues and cell cultures, opening biological interiors... with far-red and near-infrared fluorophores. Addgene's collection contains many self-labeling protein...10.1016/j.bbamem.2013.09.005  More resources from Addgene Live and Let Dye: Self-Labeling Protein Tags Fluorescent...cellular components. Some dyes target specific organelles like mitochondria, and different dyes can result...

  15. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ... and do not necessarily represent the views of Addgene. Boston, MA, USA 42.3601°N 71.0589°W – Alina Chan...I was also incredibly impressed with how well organized the march was, as well as the friendly atmosphere... and @SciCATSyvr. Additional Resources on the Addgene Blog Read Our Previow Post on the March for Science...

  16. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...101: Validation Resources on addgene.org Addgene's Antibody Guide Addgene's Antibody Protocols...efforts and information provided by external organizations like the Human Cell Differentiation Molecules...10.1038/s41598-019-55133-7 More resources from the Addgene blog Introduction to Gating in Flow Cytometry Antibodies...

  17. CRISPR Guide

    Type
    Collection
    ...window) . Addgene Website & Blog References Website CRISPR plasmids and resources at Addgene CRISPR 101...cell lines Viral service at Addgene Viral plasmids and resources at Addgene Blog CRISPR topic page How ...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science...knock out target genes in various cell types and organisms, but modifications to various Cas enzymes have...You Use a CRISPR Library? CRISPR libraries from Addgene are available in two formats: as liquid DNA, or...be added to detect the presence of infectious organisms (like SARS-CoV-2 ) and genetic mutations. Similar...located. The majority of the CRISPR plasmids in Addgene’s collection are from S. pyogenes unless otherwise...

  18. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    ...plasmids used for these methods can be found on Addgene’s stem cell page. On this page, you can also find...transdifferentiation factors are directly injected into the organ of interest for in vivo transdifferentiation. Multiple...PubMed PMID: 18940730. Additional Resources on the Addgene Blog Stem Cell Models for Disease & Open Science... can be used for gene delivery Resources on Addgene.org Visit our Stem Cell Pages Find Viral Vectors...

  19. Molecular Biology Reference

    Type
    Guide
    ... is the platform Addgene primarily uses for sequencing plasmids deposited at Addgene. The actual process...Plasmid Backbone Plasmids 101 series on the Addgene blog Addgene Protocols Content last reviewed: 22 October... A, T, C, and G, respectively). The bases are organized into a right-handed, double-stranded helix. To...the instructions to make every protein within an organism. Before proteins can be translated, the DNA is...can drive gene expression in a wide variety of organisms, including plants, worms, mice, and cultured human...use in an experiment. Plasmid Type Description Addgene Resource(s) Cloning Plasmids Used to facilitate...coli strains below and recommend checking out Addgene’s blog posts about common E. coli lab strains and...

  20. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...FAQs, Le Cong worked with Addgene Senior Scientist, Matt Ferenc, to organize the CRISPR forum's most popular...Additional resources on Addgene.org CRISPR guide CRISPR protocols by Addgene depositors gRNA design software...homology of the protospacer followed by PAM. See Addgene's CRISPR resources page for gRNA design software...scientific community and sharing these resources with Addgene. Thank you, Le! This blog post was updated to include....cell.2013.08.022  Additional resources on the Addgene blog CRISPR Cheat Sheet Build Your CRISPR Vocabulary...
Showing: 381 - 400 of 466 results