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We narrowed to 755 results for: rest

Showing: 381 - 400 of 755 results

  1. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    ...statistical methods, omission of some data, etc.  The firestorm that followed implementation of the policy comprised...Lastly, data is our raw product. If we don’t take interest in how it is released, we will be in danger of... publishers and funders. Clearly, it is in our interest to ensure that our data are reproducible and useful...

  2. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...necessary for getting the plasmid express our gene of interest. This element is responsible for initiating the...role in determining where and when your gene of interest will be expressed.      The RNA polymerase...results or even kill your cells if your gene of interest is toxic. To combat this, scientists have created...

  3. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...activation motif (ITAM). By fusing ITAM to an RTK of interest and co-expressing a cytosolic ZtSH2 reporter, ...that pYtags can be generalized to other RTKs of interest, they create sensors for FGFR1, EGFR and ErbB2...KRaION potassium sensors Whatever your analyte of interest, it’s important to choose a biosensor that has...

  4. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...that’s what keeps things interesting. And hopefully they’ll be just as interesting as the finished product...partially reopened. But there were still many restrictions in place, most importantly, masks.  Besides ...

  5. March for Science

    Type
    Blog Post
    ...together as an interest group (15) While marchers are a group with one unifying interest (the advancement...thought of as just another party. Additionally, interest groups are often pandered to when strong or overlooked...

  6. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...Google.slides or Powerpoint file right now to capture interesting key results, striking images, creative ideas,... write itself when the time comes. 5. Choose interesting content Talk about something you know a lot about...commercialized, just talking about the possibility is interesting. Check out Joanne's Reddit AMA 6. Practice, practice...

  7. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...multiple exons that, when deleted through NHEJ, will restore the dystrophin reading frame. gRNAs bind in intronic...this result, Nelson et al. find that dystrophin restoration is maintained for at least six months. Future..., Olson EN. Postnatal genome editing partially restores dystrophin expression in a mouse model of muscular...

  8. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ..., please refer to the paper. Why might one be interested in state machine technology? Biological research...and/or sortable reporters in certain states of interest. For example, in Figure 2 below we present a two-input...researcher in the Lu Lab at MIT. He is broadly interested in biocomputing and biological circuits.     ...

  9. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...million results (Here’s the top one, in case you’re interested). “But I’m an introvert!” is not an excuse. Every...someone else on that team is putting their own self-interest ahead of the team’s goals, then that team is destined...other person really is acting against the team’s interests or not; once that seed of distrust is planted,...

  10. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...able to knock-in a 2xFLAG epitope into my gene of interest, I encountered low efficiency (0.13%) and laborious.... Figure 1: In the background are 504 restriction digests of PCR products used to identify a single...CRISPR plasmids page. Future perspectives It is interesting to note that recipients for the 2015 Breakthrough...

  11. Important Considerations When Using AAVs

    Type
    Blog Post
    ...chemogenetic/optogenetic constructs into our cells of interest. Although they have a small packaging capacity...chemogenetics vs. optogenetics) I’m particularly interested in using chemogenetic and optogenetic AAV-based...2001): 1248. PubMed PMID: 11509958. 8. Ferrari, Forrest K., et al. "Second-strand synthesis is a rate-limiting...

  12. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...to a fluorophore. When paired with a protein of interest, these tools provide an easier means to identify...of cellular components, typically a protein of interest. Fluorescent dyes and ligands vary in their chemical...fluorescent ligands can elicit varying degrees of β-arrestin-2 recruitment to receptors, indicating their agonistic...

  13. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...focus fluorescence from reaching a detector or restricting fluorescence to specific excitation volumes to...reduction in signal intensity. 2-Photon microscopes restrict excitation (and bleaching) to a single focal point... a large axial range. 2-photon imaging, which restricts excitation and photobleaching to the focal plane...

  14. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...transposase. The donor plasmid contains the gene(s) of interest flanked by terminal repeat sequences, which the...plasmids that express the reprogramming factors of interest. The advantages of this method are that it’s relatively...KLF4 LIN28 Check out these plasmids if you’re interested in Tet-inducible lentiviral expression of OSKM...

  15. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ...Diagnostic Restriction Digest Diagnostic Restriction Digest You may also like... Restriction Digest of ...another. A diagnostic restriction enzyme digest takes advantage of the fact that restriction enzymes cleave ... your plasmid of interest (see below for recommended amounts) Appropriate restriction enzyme (see manufacturer's...Orientation by Restriction Digest If you clone an insert into a vector by a single restriction enzyme, you...DNA Agarose Gel Electrophoresis Introduction Restriction enzymes are naturally occurring bacterial endonucleases...enzymes and the unique sites they recognize, restriction digests have become the most widely used method...cleave DNA at specific sequences called restrictions sites. Often, the size of the plasmid insert and vector...

  17. Podcast: A Malawian Professor's Path to Biotech Research

    Type
    Blog Post
    ...Michigan State University with the backing of a prestigious Fulbright Fellowship. Dr. Kwapata now works as...genetics and biotechnology for Horticulture and Forest Crops at the Lilongwe University of Agriculture...

  18. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    ...and country. This information can be of personal interest to the depositor, but it can also be valuable ...annual reports. While the raw data is, of course, interesting to parse through, aggregation of this information...

  19. Interview with Bart Newland, Addgene’s Newest Board Member

    Type
    Blog Post
    ...move from microbiology to law? How did you get interested in pursuing law? BN: Life science was always ... general counsel at a small start-up is very interesting. It’s very varied. There’s IP work to do, general...
Showing: 381 - 400 of 755 results