...location based on a co-expressed gRNA-defined target sequence. DSBs are preferentially repaired in the cell ...

...specific location based on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell ...

...specific location based on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell ...

...considered "scarless", as the transposon homology sequences are integrated along with the donor DNA. The two...

...assessment, sterility testing, and viral genome sequencing (VGS). In-stock AAV preps are distributed as ...

...specific location based on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell ...

...testing, sterility testing, and viral genomic sequencing to confirm the identity of the packaged DNA and...

...expression, Tanenbaum et al. modified the GCN4 sequence to increase its alpha-helical structure and stability...

... CRISPR 101: Validating Your Genomic Edit and Sequencing Options for CRISPR Genotyping Day 5: Delivery...

...tropism. Introducing changes and/or short peptides sequences into these variable loops is one method for changing...

...complete or near-complete excision of the stop sequence. In contrast, Dre recombinase, the close relative...

...have figured out how to download a bit of virus sequence to enable them to recognize and fight it forever...

...gRNA(s) to guide the Cas9 protein to your target sequence, as well as design and clone in the homology arms...2A-PuroR cassette, flanked by microhomologous sequences and gRNA target sites. The first deposited PITCh...

...plasmid collection contains codon-optimized ORF sequences for human SLCs in Gateway-compatible pENTR vectors...

... (2016). Expression mediated by three partial sequences of the human tyrosine hydroxylase promoter in ... J., & Kügler, S. (2017). A MicroRNA124 Target Sequence Restores Astrocyte Specificity of gfaABC1D-Driven...to read your experimental output; and (4) the consequences to your experiment if there’s off-target expression...

...searching for. Antibodies may recognize a linearized sequence of amino acids, or only in their 3D conformation...recombinant antibodies are plasmid-based. Their sequences can be optimized for specificity and sensitivity...

...protocols, find software for gRNA design and deep sequencing analysis, discover links to CRISPR forums, and...

...Unifying the known and unknown microbial coding sequence space. eLife. (2022) 11:e67667. DOI: https://doi.org...

...saturating mutagenesis on 21 positions in the protein sequence of the predecessor GEVIs (called ASAP 1 and 2)...

...the names imply, PAM-out designs have the PAM sequences on the extremes of the targeted region, whereas...