CRISPR Plasmids - Xenopus
Type
Collection
...location based on a co-expressed gRNA-defined target sequence. DSBs are preferentially repaired in the cell ...
We narrowed to 538 results for: sequence
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CRISPR Plasmids - Zebrafish
TypeCollection...specific location based on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell ... -
CRISPR Plasmids - Parasites
TypeCollection...specific location based on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell ... -
AAV Viral Preps
TypeCollection...assessment, sterility testing, and viral genome sequencing (VGS). In-stock AAV preps are distributed as ... -
CRISPR Plasmids - Tagging
TypeCollection...gRNA(s) to guide the Cas9 protein to your target sequence, as well as design and clone in the homology arms...2A-PuroR cassette, flanked by microhomologous sequences and gRNA target sites. The first deposited PITCh... -
CRISPR Plasmids - Double-Strand Break (Cut)
TypeCollection...specific location based on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell ... -
CRISPR Plasmids - CRISPR Transposases (CAST)
TypeCollection...considered "scarless", as the transposon homology sequences are integrated along with the donor DNA. The two... -
Resolute Plasmid Collection
TypeCollection...plasmid collection contains codon-optimized ORF sequences for human SLCs in Gateway-compatible pENTR vectors... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog Post...control over gene expression that go beyond genomic sequence. Dysregulation of the epigenome (the sum of epigenetic...epigenetic modifier could be targeted to specific DNA sequences. Non-editing CRISPR applications direct catalytically... -
Easing the Protein Purification Process with pCri
TypeBlog Post...subtilis, contains the MISTIC (membrane-integrating sequence for translation of integral-membrane protein constructs... -
CRISPR Plasmids and Resources
TypeCollection...protocols, find software for gRNA design and deep sequencing analysis, discover links to CRISPR forums, and... -
Addgene is Expanding Our Viral Vector Service!
TypeBlog Post...testing, sterility testing, and viral genomic sequencing to confirm the identity of the packaged DNA and... -
SunTag and Fluorescent Imaging
TypeBlog Post...expression, Tanenbaum et al. modified the GCN4 sequence to increase its alpha-helical structure and stability... -
New CRISPR Web Resources and #12DaysofCRISPR Recap
TypeBlog Post... CRISPR 101: Validating Your Genomic Edit and Sequencing Options for CRISPR Genotyping Day 5: Delivery... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog Post...tropism. Introducing changes and/or short peptides sequences into these variable loops is one method for changing... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog Post...complete or near-complete excision of the stop sequence. In contrast, Dre recombinase, the close relative... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog Post...have figured out how to download a bit of virus sequence to enable them to recognize and fight it forever... -
CRISPR Plasmids - Base Edit
TypeCollection...work in a very narrow window proximal to the PAM sequence, some base editing systems create a wide spectrum... -
Brain Initiative Collection
TypeCollection...dendrites under the control of internal ribosome entry sequence in a viral vector Hillel Adesnik 111066-AAV1 pAAV-hSyn-dLight1.1...Cre-dependent expression; soma localization targeting sequence GENIE Project 172908-AAV1 pGP-AAV-syn-FLEX-Ace2N...Cre-dependent expression; soma localization targeting sequence GENIE Project 174007-AAV9 pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST...and ChrimsonR are separated by cleavable peptide sequence P2A. Mark Histed 179459-AAV1 pAAV-EF1a-DIO-JEDI... -
Viral Vectors 101: AAV Variables That Matter
TypeBlog Post... (2016). Expression mediated by three partial sequences of the human tyrosine hydroxylase promoter in ... J., & Kügler, S. (2017). A MicroRNA124 Target Sequence Restores Astrocyte Specificity of gfaABC1D-Driven...to read your experimental output; and (4) the consequences to your experiment if there’s off-target expression...