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...gene-targeting capabilities of full-length SpCas9. This set of plasmids includes AAV-Cas9C-VPR for targeted gene...genes in human cells using compact expression cassettes. Sci Rep 5: . https://doi.org/10.1038/srep10777...

...frogs, mice, and even humans. It binds to a specific set of DNA-binding transcription factors to repress the...

...If your lab owns a liquid handling robot, you can set it up to automate the reagent transfer and the restriction–ligation...

...your “gating strategy.” This series of markers are set up sequentially, from broadest to most specific marker...Subtypes of immune cells are often defined by a subset of the markers used to identify them, such as “...

...in the best-case scenario [7]. In the clinical setting where patient blood samples are limited, such low...by Kusumika (Kushi) Mukherjee, a Postdoc at Massachussetts General Hospital. A little over a decade back...Mukherjee is currently a postdoctoral fellow at Massachusetts General Hospital with research interests in ...

...team members only, under the specific permissions set for every member of your team. Do you need to make...

... (Cong et al, Mai et al) we were very excited to set up a CRISPR-based editing platform in our lab. Our...

...forums on particular topics. Volunteer moderators set the rules for these forums and try to keep discussions...

...Tempurature: Analyze your expression conditions by setting up a small-scale expression experiment to test ...StrR, TetR) Expression of insoluble proteins  Rosetta2 (DE3)* Chloramphenicol (pRARE) Good for “universal... plasmid contains a chloramphenicol resistance cassette for positive selection and a p15A origin of replication...

...PMCID: PMC3748948.  Khetrapal, Varnica, et al. "A set of powerful negative selection systems for unmodified...expression of a gene with an antibiotic resistance cassette. This leaves a “scar” in the chromosome, sometimes...vector contains sacB, an chloramphenicol resistance cassette, and the R6K origin of replication to allow replication...pDS132 (Philippe et al., 2004): Replaced the sacB cassette with one of three toxins from the Khetrapal paper...medicine and clinical infectious diseases at the Massachusetts General Hospital where he is now an attending...

... this skill that you could strengthen. The right set of transferable skills, coupled with your technical...know you are bringing this asset to your job, you can become a valuable asset yourself.     Fig... point of view and can’t understand why they’re upset, conflict resolution is likely not one of your strengths...

...range. With this in mind, Ed Boyden and colleagues set out to engineer a potassium ion biosensor with a ...

...with potentially harmful mutations in therapeutic settings. Silencing of a target gene is achieved through...; font-weight: normal; text-decoration: none","font_set":"DEFAULT"},"button_hover_color":{"color":"#0052a9...

...mutagenesis is especially concerning in clinical settings, as evidenced by retroviral gene therapy trials...

... uses Cre to trigger the inversion of two tandem sets of genes aligned head-to-head and the deletion of... same XFP transgene in an adult fly. As a stop cassette is located directly upstream of the fluorescent...

...co-factor, (iii) easy visualization with standard filter sets on a fluorescence microscope, and finally (iv) good...

...bidirectional USA-Europe professional collaborations, setting up bases and contacts with Spanish laboratories...Universities in USA and Europe (Harvard University, Massachusetts Institute of Technology, Boston University, ...

...microscope stage) Reagents None needed Procedure Set up your microscope by removing any protective cover...

...each individual target is stained correctly Image a set of samples where you label only one target in each...

...antibody is used to visualize the proteins. In this set-up, multiple secondary antibody molecules bind the...another great option to enhance resolution. In this setup, gels have an increasing range of acrylamide content...