...more standardized methods for evaluating CRISPR technologies, including the ever important on/off-target ... are aimed at improving translation of CRISPR technology, but they would also bring benefits to researchers...pre-clinical research. However, standard terms and techniques are not enough to move the CRISPR field forward...

...Additional Posts on Mol Bio Techniques Find Additional Posts on Plasmid Technologies Resources at Addgene.org...Plasmids in the Repository Learn about New Plasmid Technologies in our Hot Plasmid Articles ...

...with people, nurturing networks, as much as with technical knowledge. I leverage a breadth of knowledge to...I am constantly teaching – often explaining a technical piece of research to others outside that specialty... thinking about, for example, how to use new technologies to reach the right audiences and how open access...

...the less acknowledged perks of scientific and technical training is that these educational paths prepare...encompasses millions of choices including pharma, tech transfer, management consulting, science communication...Small Company Big (e.g. Pharma) Small (e.g. Biotech) Develop expertise areas quickly Jack/Jane of...

...how they function. Adapting Toehold Switches to Detect Zika Virus The RNA-based toehold switches described.... These toehold switches can be used to quickly detect the presence of Zika virus. Read this post from...Combinatorial Genetic Screening CombiGEM is a technology that can be used to quickly barcode a set of ...

...excited about our new partnership with Allele Biotechnology which allows researchers to deposit plasmids...mTFP1 and mWasabi, as fluorophores from Allele Biotechnology that now can be deposited at Addgene. What makes...colleagues from The Scintillon Institute, Allele Biotechnology, Florida State University, Indiana University...

...lentiviral vector plasmid in Escherichia coli Stbl3. 3 Biotech 3:61–70. https://doi.org/10.1007/s13205-012-0070...vectors using reduced-genomeEscherichia coli. BioTechniques 43:466–470. https://doi.org/10.2144/000112585...4847–4851. https://doi.org/10.1073/pnas.77.8.4847 Trepotec Z, Geiger J, Plank C, Aneja MK, Rudolph C (2019...

...present in prokaryotes, and some of these proteins protect against invading DNA. In 2014, Swarts et al. characterized...the Natronobacterium gregoryi Argonaute.” Nat Biotechnol. Epub 2016 May 2. PubMed PMID: 27136078. Find...Resources on Addgene.org Check Out Other Argonaute Technologies from the Doudna Lab Browse the CRISPR Collection...

...many developments in science and the plasmids, technologies, and researchers behind them. As part of our...started using plasmids and the revolutionary CRISPR technology in their own labs. We’re overjoyed to provide...will be sure to keep our eyes open for any new technologies that will bring about the next revolution in...

...individuals in their 20’s who’ve built multibillion dollar tech companies using only laptops and some server space...our network.  We started with the idea of a biotechnology investment fund that would apply a percentage... biotechnology...

...type; however, their usage has been limited by technical problems. For instance, it’s much more difficult...proteins and reporters Previous Protein Delivery Techniques: Introduction of macromolecules into cultured...homing an artifact? Lundberg M, Johansson M. Nat Biotechnol. 2001 Aug;19(8):713-4. PubMed.  ...

...Escherichia coli or Saccharomyces cerevisiae with few techniques that are easily transferrable across host species...for rational, straightforward design. Since the technique relies on base pairing, it has promise to be host-independent...engineering, this is such a powerful and flexible technique that it should be on the radar of every molecular...

...institute. A common reason is hands-on training in a technique that only a few labs have expertise in. Visiting...sure you read any relevant literature for the techniques you will be performing to familiarize yourself...help you with your research and teach you new techniques. Show up on time (don’t make your bench mentor...

...AAVs for serotype testing jGCaMP7c for calcium detection PSAM4 GlyR for chemogenetic inhibition Flp expression...) AAV-hSyn-EGFP Biosensor AAV The ability to detect the neurotransmitters and other bioactive molecules...sensor. This “contrast” variant provides improved detection for tracking large populations of neurons in wide-field...

... for gene editing. They named this new array architecture the DAP array, short for “drive-and-process....payloads. Different from existing multiplex array architectures, including previous tRNA-gRNA arrays, that rely...Comparison of previous representative dual-gRNA architectures with DAP array   Qichen Yuan is a PhD...

...turns 10 years old, it’s easy to forget that this technique isn’t limited to neuroscience. In fact, precise... method for the computational problem of edge detection. Tabor et al. subsequently engineered a second...publication at Addgene. A synthetic genetic edge detection program. Tabor JJ, Salis HM, Simpson ZB, Chevalier...

... and open source software, hardware, wetware, technology development, etc). Second, the potential hire...job satisfaction (especially for scientists and technical people) is linked to engagement and access to ...you would like to head for a career in pharma or biotech, this is one case where the topic of your research...

...acids in the domain). Yan et al. from Arbor Biotechnologies found that RspCas13d accessory protein RspWYL1...functional in multiple species (read Arbor Biotechnologies' press release about their Cas13d deposit here...Addgene. Learn more about research at Arbor Biotechnologies. Additional Resources on the Addgene Blog:...

...visible part behind these changes: the backend technology that powers the new website search. Tackling ... resources, blog posts or reagent items.  The technology behind Addgene’s new search  Like millions of...PI labs and institutions. There are many more technical details that make our new search engine unique...

...fluorescent molecule. A higher brightness increases the detected signal and therefore high brightness is an important...brightness that does not fold well or cannot be detected in a microscopy setup has zero practical brightness...excitation wavelength, available emission filters, and detector sensitivity) and the biological system (temperature...