We narrowed to 547 results for: try
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TypeBlog Post...neuroscience and is available to both academic and industry scientists. The collection of antibodies available...2019), validated in both western blot and immunohistochemistry. Additionally, many of the hybridomas from...
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Antibodies 101: Epitope Tags
TypeBlog Post...tags are frequently used for western blot, flow cytometry, and immunofluorescence . However, protein purification...tag is ideal for live cell imaging, FRET, flow cytometry, or immunofluorescence where an antibody can’t... -
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog Post...Previous work by Stoneham et al. demonstrated that the entry of AAV2 into target cells is blocked following sucrose...adeno-associated virus/phage." Journal of Biological Chemistry (2012): jbc-M112. PubMed PMID: 22915587. PubMed... -
Creating accessible biology activities in schools with BioBits
TypeBlog Post...together in chemistry to see what happens, but there isn’t really an equivalent of a “chemistry kit” for ... -
Celebrate National Book Month with Addgene
TypeBlog Post...had a bachelor’s degree or a PhD, majored in Biochemistry or International Finance. A book club could ...lapsed. Baker’s novel reignited my appreciation of poetry, and I’ve been reading a poem a day for the last... -
The MTA at Addgene
TypeBlog Post...have the option of distributing under Addgene’s Industry MTA (IMTA), which allows the plasmids to be distributed...distributed for research use only to industry-based scientists. Some organizations choose to share their... -
Cultivating Community Science at BosLab
TypeBlog Post...no longer just for those working in academia or industry. At BosLab, we identify ourselves as part of a...creations that may not be pursued in an academic or industry lab. Mrina Nikrad, Ph.D., is an expert in microbial... -
3 Challenges in Plant Synthetic Biology
TypeBlog Post...in 2007. Nikolai trained as a post-doc at the Biochemistry Department at the University of Manchester as...receiving her bachelors in Molecular Biophysics and Biochemistry from the Illinois Institute of Technology in... -
Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages
TypeBlog Post... anyway, including the entire faculty of the biochemistry department at the University of Massachusetts...changed since the injunction”. Postdocs around the country have now begun to speak up on this issue. These... -
RNA Extraction Without A Kit
TypeBlog Post... acid from sources enriched in ribonuclease. Biochemistry 18:5294–5299. https://doi.org/10.1021/bi00591a005...Thiocyanate–Phenol–Chloroform Extraction. Analytical Biochemistry 162:156–159. https://doi.org/10.1016/0003-2697... -
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards
TypeBlog Post...Physiology. Her research focuses on the neural circuitry involved in mechanical allodynia, a condition ... light activation and CNO silencing. (D) Immunohistochemistry verification of the dual virus expression... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog Post...directly visualized with fluorescence or immunohistochemistry methods, these proteins are routinely fused... also be identified using antibodies and immunohistochemistry techniques. Thus, these proteins provide... -
The Future of Research Symposium Boston 2015
TypeBlog Post... has a compulsory internship to be taken in an industry, regulatory, or clinical environment. It remains...there for STEM graduates and postdocs in academia, industry and other areas of employment? How many graduates... -
Plasmids 101: Golden Gate Cloning
TypeBlog Post...ligated into a vector containing those overhangs. Entry DNA overhangs may be present in the original plasmid... 30-minute reaction. The destination vector and entry vector(s) are placed in a single tube containing... -
Engaging with science and society at pgEd
TypeBlog Post...meetings of experts and leaders from academia, industry, community, education and government; advising...academic venues such as scientific, educational or industry conferences, as well as schools, community events... -
Rabies and Neuronal Tracing
TypeBlog Post...transmits retrogradely to first order neurons, via entry mediated by the G protein/G-protein receptor. Once...powerful tool that can be used to reveal brain circuitry, and continue to contribute answers to the complex... -
Split-BioID: An Improved Method for Studying Protein-Protein Interactions
TypeBlog Post...identifying PPIs, such as affinity purification mass spectrometry (AP-MS), lack the ability to specifically identify... purified via streptavidin pulldown. Following trypsin digest, PPIs are identified via mass spec analysis... -
Hot Plasmids February 2024
TypeBlog Post... toxicity from electroporation or poor cellular entry with other transfection methods. The Junwei Shi ...human cell lines and primary cells. Although cell entry of a Cas9 ribonucleoprotein (RNP) was limited (likely... -
Early Career Researcher Toolbox: Finding Relevant Papers
TypeBlog Post... “cited by” and references sections of a PubMed entry are great places to look for more articles on your...contain your search term. When you view a paper’s entry, the number of citations that paper has is displayed... -
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux
TypeBlog Post...also culminated in a joint American Society for Biochemistry and Molecular Biology/ FoR Hack Day during which...FLSA) which mandates that all workers across the country either be paid more than $47,476 or be eligible...