We narrowed to 532 results for: abo.3

... as their orientation relative to the gRNA (5’ or 3’). In some cases, there is no requirement, which is... spCas9 isn’t always the winner!).   Fig. 3 – Comparison of different Cas enzymes using CasPEDIA...you are a structure junky who just wants to learn about Cas domains. CasPEDIA either directly has this information...

...very little bit about hiring when we need to do it quickly. Hiring is hard but as a 3 year Boston Globe...like The Atlantic. We’ve been in documentaries in 3 languages (Japanese, German, and English) and even...keep spreading the word about materials sharing, but we don’t have to worry about getting support from depositors...of course always being vigilant about resources, I’m not worried about the stewardship of this in the ...Addgene is about half of what is standard in most sectors. The Addgenies support a high collaboration, high...on this. Addgene’s success is about the Addgenies. I never worry about their dedication, excellent intentions...more about Addgene Resources on the Addgene website Find plasmids for your research  Learn about our ...

...microenvironments made of?  A tumor’s genesis is divided into 3 phases: initiation, progression, and metastasis. The...microenvironment’s physiology is deeply connected to these 3 stages and contains multiple cell types: fibroblasts... related blog posts Learn about our cancer collection pages Read more about the tumor microenvironment...the tumor. How do microenvironment enzymes and metabolic reprograming influence tumors development? Being... vice versa, let’s take a look at cancer cell metabolism. Cancer cells promote oxidative stress leading...of cells affect tumor progression, enzymes and metabolism reprogramming aren’t the whole story. It’s essential...particularly interested in science journalism and writing about biomedical issues. Follow her on Twitter @BrbaraPinho6...

...way is not necessarily easy, so we came up with a 3-part strategy: Train: Nearly every new Addgene employee...communication has helped Addgene move from completing 2-3 large projects a year, on average, to completing 8...differently based on their audience. How you talk about your experiments to your PI, your colleagues, or...have cross-team projects within your own lab, collaborations with other scientists within and outside your...not happen in the lab, they have parallels to collaborative lab work. Corralling context and junking jargon...difficult to know what kind of plate they were talking about during the meetings. And “plate” certainly wasn’... a lot on context. New people in the lab or collaborators will not have the context you do, which means...

...workflow. B) Detail of spacer peptides (3HB: 11-nm 3-helix bundle; SAH: 60-nm single alpha helix) and target...optimized PASSIGE plasmids here!     Figure 3: Schematic of the PASSIGE strategy. First, twin prime.... When the prime editor releases the DNA, the two 3’ flaps containing the edited sequence anneal with ...transcriptional silencing (Nuñez et al., 2021). Now, in a collaboration with the Vallabh/Minikel Lab, the Weissman Lab...

...they observed that 41/42 embryos were non-mosaic at 3 days post fertilization. Since Ma et al. designed ...proper development. Ma et al.’s experiments showed a 3% mosaic rate, and this rate would need to be much,...August 2nd in Nature gives us even more to talk about, as it represents another step towards CRISPR germline...impossible with current tools, thus quieting some fears about the creation of “designer babies.” The differences...of individuals with varied backgrounds. Talking about this study with non-biologist friends Like me, you... Blog Check out Our New CRISPR Topic Page Read about High Fidelity CRISPR Variants Target Multiple Genes...

...manuscript review/submission process at JEI. Chapter 3 - JEI target audience and their current readership...working in a lab for the first time, and thinking about the future. Many thanks to our guests,Olivia Ho-Shing...resources Use our Plasmids 101 eBook to teach students about basic plasmid biology Use or CRISPR 101 eBook to...

...a truncated version of the original Tol2 and has ~3-fold increase in transposition activity  Tol2 transposon...the QCascade DNA-targeting module with the crRNA, 3) a transposase complex made up by three transposase... up to 10 kB in size in bacteria.   Figure 3: INTEGRATE system for RNA guided transposon insertion...Addgene blog Learn about the INTEGRATE system for bacterial genome engineering  Read about the Sleeping Beauty...DNA transposons are most commonly used in the laboratory for genome manipulation. When transposons are...Applications of transposons Now that you’ve learned about some of the popular transposon systems, let’s take...2020) Jump around: transposons in and out of the laboratory. F1000Res 9:135. https://doi.org/10.12688/f1000research...

...pulse of 500 nm light (turn on psRaf1) followed by 3 seconds of 400 nm light (turn off psRaf1). After this...is responsible for this MEK1/2 negative feedback. 3. Studying signaling pathways in vivo Expressing photoswitchable...Additional Resources on the Addgene Blog Learn More about Photoactivatable Fluorescent Proteins Control Protein...

...produce yellow, cyan, or magenta light. A gRNA with all 3 distinct hairpin sequences produces white light. Created...Find the plasmids from this publication at Addgene. 3. Ma, Hanhui, et al. “Multiplexed labeling of genomic...Additional Resources on the Addgene Blog Read More about Fluorescent Protein-Based Tools Learn Some of the...