We narrowed to 1,015 results for: LEA

...It’s clear that CRISPR-Cas9 technology has really changed the game for anyone looking to quickly and ... positive selection screening. They and their colleagues including Feng Zhang used the GeCKO library to...CRISPR libraries developed by MIT's Tim Wang and colleagues, including David Sabatini and Eric Lander, comprise...

...There’s a steep learning curve for Inkscape, but there are lots of resources for learning the software. ...media for scientists Find science career blog posts Learn about other lab software ...

...cells expressing Fcγ receptors binds to IgG and release cytotoxins that kill the target cell. If the pathogen...antibodies and eosinophils, where the eosinophils release cytotoxins via degranulation.  Antibody-dependent... as phagocytosis.  Other functions We’re still learning about Fc effector functions and all that antibodies...

...scientific data depends on validated reagents along with clear documentation, so we were excited when Amos Bairoch... cited. If the information seems incomplete or unclear, Bairoch follow up via email to the authors of ... you’re excited about this resource and want to learn more, check out the Cellosaurus FAQ! References...

...CRISPRi). A) Lentiviral vector design. B) Strategy for leak-free transcriptional control. C) Iterative transduction...activation in plants.  FnCas12a, unlike other Cas12a nucleases identified, functions at temperatures up to 43...The guides are optimized to maximize on-target cleavage and minimize off-target effects.   New from ...

...Product Development Manager at eScience Labs. To learn how Caitlin got into scicomm and landed her first...series! Additional Resources on the Addgene Blog Learn more about alternative science careers here Interested... for scientists here Resources on Addgene.org Learn more about careers at Addgene ...

... anti-phage CRISPR effect observed in bacteria. Learn more about the different CRISPR applications in ...23256573. Additional Resources on the Addgene Blog Learn More About the Addgene-Seeding Labs Plasmid Grant... CRISPR for plants Bacterial Expression Systems Learn about Global Plasmid Distribution ...

...require the use of specific sequences. Read on to learn how easy this process is and to get tips for applying...not well understood, it is believed that the exonuclease activity of the polymerase generates sticky ends...fidelity DNA polymerases like phusion. An 18-cycle PCR leads to the most colonies after transformation. When ...

...asking what approach will I take and what can I learn, learning the difference between good and bad data, and...establishes your professional brand. It offers colleagues insight into your interests and will determine...

...viral vectors. Here are some of the AAV we have released in the last few months. You can also browse our...that govern such activities as neurotransmitter release is vital in studying neural networks. We have expanded...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...

...suggesting that outcomes are already determined by at least day 13. However, distinct transcriptional states...335547. Additional resources on the Addgene blog Learn more about induced pluripotent stems cells (iPSCS...delivery of reprogramming factors for generating iPSCs Learn how fluorescent proteins help researchers study ...

... nearing deadline (an animated video depicting nuclear fission; a podcast with a genetics researcher on...condensed into two discernible paths. The first leads into the world of industry. Along this road, science...Understanding just how the public utilizes insights gleaned from scientific research is, after all, one of ...

... A diagnostic digest is one of the easiest and clearest ways to determine if your sample has recombined...extraction to isolate the full, intact plasmid. Please see our gel extraction protocol for more details...more molecular biology and viral vector protocols Learn about the common lab E. coli strains Find tips for...

...antibodies are supplied with no sequence information, leading to problems with reproducibility and an inability...to polyclonal or monoclonal antibodies. You can learn more about recombinant antibodies in this blog post...Addgene blog Check out our Antibodies 101 blog post Learn more about plasmid-based recombinant monoclonal ...

...hope that this provides a clear overview of what you need to submit data. Please note that the data hub ...generated with a caption. For microscopy images, please include channel imaged, a color legend for probes...

...ever like to write about a recent plasmid deposit please sign up here. Listen to the Hot Plasmids Podcast...technologies based on polymerase processivity. Please find the MarathonRT plasmid here! Zhao C, et al...used to target any protein fused with GFP, YFP, mCerulean, or other related fluorophores. Find all of the...

...with lentiviral preps for the delivery of Cas9 endonuclease, Cas9 nickase, Cas9 activators, Cas9 repressors...expertise you’d like to contribute to the blog, please email us and we’ll get you writing!     www.addgene.org... Service or any of the materials we distribute, please send an email to [email protected]. Your questions...

...been an exciting year for the Addgene blog. We’ve released over 80 posts on a diverse array of topics pertinent...Plasmids 101 2nd ed, the 3rd edition has now been released), and have started providing recorded interviews...Resources at Addgene Browse Our CRISPR Resources Learn More about Viral Vectors Browse the Repository ...

...genome engineering methods for C. elegans were released, signaling a new era in C. elegans research. Homology...on the first attempt for 6/7 genes. The data are clear - the SEC-based system is both simple to use and...rapid, and user-friendly system represents another leap forward for the CRISPR/Cas9 revolution!   References...

...move, such as T lymphocytes. Griesbeck and his colleagues began by isolating a calcium-binding protein,... also points out that the approach he and his colleagues took to optimize sensitivities can now be applied...calcium, requiring greater sensitivity. He and his colleagues came up with a solution: they devised self-associating...

...ever like to write about a recent plasmid deposit please sign up here.        New toolkit for N or C terminal...widely used genome editing tool. However, Cas9’s endonuclease activity can be limited by the need for a specific...and follow the DNA sequence targeted by the Cas9 nuclease. The Streptococcus pyogenes Cas9 (SpCas9) requires...

...dysregulation. A targeted TF screening approach can not only lead to a better understanding of disease onset, but ...isoforms. Due to the size of the collection, we’re releasing individual plasmids in batches. If there’s something....2017.016. Additional Resources on the Addgene Blog Learn More about Lentivirus Figure out How to Get the ...

...depends on things being organized, tracked, and clearly communicated. And that’s where MTAs, and similar...how the shared reagents should be used. Setting clear expectations from the beginning makes the sharing...sharing process smoother, more consistent, and more pleasant for everyone, which in turn encourages people ...

...for reproducibility because mistakes in this can lead to wasted time or retractions. Using a catalog number...Bauer (Addgene plasmid # 87155 RRID:Addgene_87155)” Please note that we only provide recommendations for how...Check out this post on Scientific Reproducibility Learn about the anatomy of a plasmid page at Addgene ...

...results as soon as the gels ran.” Dr. Ellis tries to lead his team as a scientists and a friend. George Church...3 for tips on how to develop your role as a lab leader, and then on to Chapter 4 for advice on staffing... labs. Further Reading & Resources EMBO Lab Leadership Courses HHMI Resources for Early Career Scientists...

...receptor-transcription factor fusion. TEV protease cleave the transcription factor from the receptor, which... in chemogenetics  While there’s still a lot to learn about GPCR signaling, researchers in the meantime...02775-8 Additional resources on the Addgene blog Learn more about TRUPATH Read our quick guide to chemogenetics...

...forward-looking. Embrace it, and let your personality leap out of the page. Finally, as previously mentioned...the recommender knows you the better, and their leadership status in their current field of expertise augments...But you create a network of friends, and future colleagues that can help pull one another up while you are...

...search tool updates. Now, let’s take a look at the least visible part behind these changes: the backend technology...engine! Additional resources from the Addgene blog Learn about other features on Addgene's website Find a...step-by-step guide to using Addgene's new search Read to learn more about why we redesigned our homepage and mascot...

...our Viruses as Biological Tools blog can help you learn about the different types of viruses and pick one..., McPhee DA, Verity E, Dyer WB, Wesselingh SL, Learmont J, Sullivan JS, Roche M, Zaunders JJ, Gabuzda ...Resources on Addgene.org Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral...

...enter the nucleus during mitotic breakdown of the nuclear envelope. Lentiviruses can transduce both dividing... small enough to enter the nucleus through the nuclear pore complex. This feature may be useful for the...stimulates the expression of transgenes via increased nuclear export. LTR Subcomponents: -U3 -R -U5 LTR...