We narrowed to 931 results for: TIM

...tracing of the performance of each gRNA multiple times within the same experiment by counting both the ...restriction enzyme digestion of single molecules in real-time. Find plasmids expressing tTALE-FP Shin et al., ...

... or yellow fluorescent protein at the time. It is 1.5 to 3 times brighter than the most commonly used ...

... be spending less time and resources resupplying our current inventory and more time providing new materials...

... experiment has not worked…again…for the seventh time….   I find that once I sit down and talk to a scientist...the midst of all these storytelling devices, we sometimes forget the most important thing about science:...

...neuroscience research and other disciplines, over time. The resource may also expand to include other affinity...collection to become more useful to everyone over time. Through our incredible community, including your...

... replicate, and the transgene will be lost. Over time, bacteria containing the recombined vector backbone...post. Check yourself before you recA yourself. Optimize Growth Conditions To reduce recombination, it ...

... making a copy of a piece of DNA and at the same time adding restriction sites to the ends of that piece...both function in the same buffer, as this will save time later In our example, we will use EcoRI and NotI...

...Addgene, with more added to the collection all the time! We’ll serve up a sampling of some of the most popular... what these libraries are capable of at the same time. Barcoding libraries Just as physical barcodes help...

...community, many cellular research tools have been optimized specifically for and in HEKs. These systems built...are immortalized, but cell health can degrade over time in culture. This can affect factors that could skew...

...gene synthesis, many researchers now use codon-optimized versions of common coding sequence features, so... in commercial plasmids were inconsistent and sometimes clearly wrong. In an effort to be rigorous, we...

... that could be transferred between cells. At the time, these extranuclear agents of heredity were thought...the widely adopted name for these elements. At the time, the use of episome seemed appropriate, especially...

...described in 1971,” McSweeney said. “But, it is still sometimes wrongly diagnosed as a seizure disorder.” In 2012... working on several genetic diseases at the same time. The limitation of budget restrained us from costly...

...compete against each other. Getting two-color stimulation has been a holy grail of the field. The problem...to try these optogenetic tools out for the first time? Boyden: There are always considerations for each...

...playing with some cards and I had a quick flashback to time spent playing a card game called “Magic”. There ...certain target cell population. Who is the winner? Ultimately, society should be! This is a collaborative game...

... four variants of these biosensors, which are optimized for specific parameters: jGCaMP7s is the most...specificity of influence measurements by reducing photostimulation of the axons and dendrites of non-targeted...

...understanding of PZA action and could potentially stimulate the development of useful diagnostic methods and...non-linear microscopy methods will be used to stimulate and manipulate neural populations in order to ...

...reproducibility because mistakes in this can lead to wasted time or retractions. Using a catalog number for identifying...are verified and identifiable to save researchers time and make their work more reproducible. While Addgene...

...body and how it works. I used to stay up past my bedtime, poring over my grandparents’ medical textbooks...communication as a PhD student Fortunately, by the time I was ready to graduate with my BSc in Anatomy, ...

...scientists of all areas who volunteered to donate their time and expertise to help them. In less than two weeks...and easily credited. And nothing stops these one-time favors from becoming starting points for long-lasting...

...voltage indicators (GEVIs) change fluorescence in real time when neurons or muscle cells transmit electrical... and conditions that can be used to monitor real time voltage changes in worms and have made these constructs...

... and develop skill in science communication over time.  Like most skills, great science communication ... to pose these why questions to yourself, but ultimately there is no substitute for feedback from others...

...How many times have you looked at a diagram depicting transcription, or DNA repair, or replication, or... Berkley Gryder. Addgene depositors or not, your time is valuable ...

...software tools for the right people at the right time. To explain a bit further, a software product manager...going to need to be a problem-solver. A lot of the time, the problem to solve is clear, but the solution...

...targeting. While promoters and AAV serotypes can sometimes be used to target tissue types, recombinases allow...next 6 months, pending quality control. For an estimate on availability date or to be notified when a ...

...changes simultaneously with 54% efficiency, about 7 times higher than ABE7.10. However, the increased editing...the ABE8e plasmids at Addgene! Why engineer and optimize base editors? Targeted evolution of naturally ...

...bogged down with distributing them and spending more time on research. What do you hope Addgene will accomplish...and participates locally in Read To A Child's "Lunchtime Reading Program" in Cambridge, MA. Additional...

...Will it be sensitive enough? No one wants to spend time screening hits that aren’t “real” or novel for their... understand the difference between the two, it’s time to check out our CRISPR library resources or our...

...usually 1-3 of the first 3 bps are modified) for optimal stability and efficacy.    Base modifications What...mechanism to turn cutting off or on at a specific time. Recent advances in gRNA modification have overcome...

...acid sequence of the antibody-encoding genes over time as the hybridoma cells divide. These changes can...meaning that there will be changes between lots over time. Specifically, the antigen-binding sites of the ...

...transgenic flies to study your favorite gene (YFG).  Sometimes, you want to use the Gal4/UAS system, but the ... as the intestines, can be dissected at various times throughout the lifespan of the fly to determine ...