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  2. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...neuroscience research and other disciplines, over time. The resource may also expand to include other affinity...collection to become more useful to everyone over time. Through our incredible community, including your...

  4. What the HEK?

    Type
    Blog Post
    ...community, many cellular research tools have been optimized specifically for and in HEKs. These systems built...are immortalized, but cell health can degrade over time in culture. This can affect factors that could skew...

  6. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ...you have only one plasmid type in your cells at a time? Do you want to make a lot of your plasmid? Is the...streaked bacteria have higher copy numbers – for optimal results always pick a single colony and do not ...

  7. Summer SciComm: Science Storytelling

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    Blog Post
    ... experiment has not worked…again…for the seventh time….   I find that once I sit down and talk to a scientist...the midst of all these storytelling devices, we sometimes forget the most important thing about science:...

  8. Plasmid Cloning by PCR

    Type
    Blog Post
    ... making a copy of a piece of DNA and at the same time adding restriction sites to the ends of that piece...both function in the same buffer, as this will save time later In our example, we will use EcoRI and NotI...

  12. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...playing with some cards and I had a quick flashback to time spent playing a card game called “Magic”. There ...certain target cell population. Who is the winner? Ultimately, society should be! This is a collaborative game...

  13. New and Upcoming Viral Vectors - September 2019

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    Blog Post
    ... four variants of these biosensors, which are optimized for specific parameters: jGCaMP7s is the most...specificity of influence measurements by reducing photostimulation of the axons and dendrites of non-targeted...

  17. Summer SciComm Series: A PhD in Science Communication

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    Blog Post
    ...body and how it works. I used to stay up past my bedtime, poring over my grandparents’ medical textbooks...communication as a PhD student Fortunately, by the time I was ready to graduate with my BSc in Anatomy, ...

  19. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ... and develop skill in science communication over time.  Like most skills, great science communication ... to pose these why questions to yourself, but ultimately there is no substitute for feedback from others...

  21. A Novice's Perspective on Neuroscience - SfN2017

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    Blog Post
    ...a talk about the different parts of the brain stimulated by stories. In retrospect, I should have expected...looking to get into the field, this is a fantastic time to do it! Additional Resources on the Addgene Blog...

  23. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...targeting. While promoters and AAV serotypes can sometimes be used to target tissue types, recombinases allow...next 6 months, pending quality control. For an estimate on availability date or to be notified when a ...

  25. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...catalog are very well-characterized. During this time, we’ve also created a variety of educational resources...the foundations for many improvements to come. Ultimately, we would like our search engine to be so specialized...

  26. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...acid sequence of the antibody-encoding genes over time as the hybridoma cells divide. These changes can...meaning that there will be changes between lots over time. Specifically, the antigen-binding sites of the ...

  29. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ... of Cas9 expression between experiments and over time (as compared to separate transductions with a single-vector...(Gi)-mCherry When using this virus for the first time, the Roth Lab recommends verifying cre expression...

  30. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...Once upon a time, not so long ago, spCas9 was the only Cas enzyme widely available and applied by researchers...better experimental fit due to this. But now, it’s time to cast aside those concerns and embrace a new resource...
Showing: 601 - 630 of 925 results