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  4. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...excited to see how this exchange of genetic tools facilitates plant science. Challenge #3: Intellectual property...in 2004, she accepted a commission in the United States Air Force. She left active duty to pursue a degree...

  6. dTAG - You're it!

    Type
    Blog Post
    ...selective small molecule binders. To overcome this limitation, we created a generalizable tag-based strategy...context. Immunoblotting is recommended, while quantitative proteomics experiments will provide the best...

  7. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...RANbody platform from the Sanes Lab overcomes this limitation and allows for the flexible design and small ...you own DNA ladders with these plasmids from Penn State Need help picking the right fluorescence microscopy...

  10. Celebrating 15 Years of Scientific Sharing

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    Blog Post
    ...We are delighted at the early outcomes and the ecstatic reception by scientists. The success of these...candidates from internal referrals. Our long reaching reputation helps us hire great people from farther away ...

  12. Antibodies 101: Secondary Antibodies

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    Blog Post
    ...sometimes assumed that the direct method is more quantitative than the indirect method. But in reality, multiple...immunoimaging assays do not allow for absolute quantitative analysis of a sample. Instead, relative quantification...

  13. CRISPR 101: Cas9 vs. The Other Cas(s)

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    Blog Post
    ...therapeutic delivery by AAV, which has strict capacity limitations. New efforts have recently been made to increase...engineering goals expand beyond generating frameshift mutations or single base pair edits, these enzymes are worth...

  15. The Future of Research Symposium Boston 2015

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    Blog Post
    ...at this event added to a growing dialogue on the state of a system that, despite all of its successes, ...Andrew Young School of Policy Studies at Georgia State University, is an expert on the academic labour ...

  16. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...the take rate of mouse xenografts after cell implantation. Their goal was to figure out how to optimize...lung cancer cell line containing an activating mutation in epidermal growth factor receptor (EGFR) with...

  17. Plasmids 101: Golden Gate Cloning

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    Blog Post
    ... that flank their DNA fragment in an inverse orientation. This allows for multiple DNA components (promoters...amplification can be used to create silent point mutations at internal recognition site(s) thus eliminating...

  21. 6 Tips for Grant Writing

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    Blog Post
    ...paper, but positive outcomes can include poster presentations, talks about your work, or even the successful...Learn How to Become a Grant Writing Professional Facilitate Career Planning and Personal Development with...

  22. Tips for Technical Support Calls

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    Blog Post
    ...scientific articles, patents, and even some doctoral dissertations. Streamline the question asking process Having...answer, consider asking to avoid unrealistic expectations. And finally, provide feedback. If you are...

  23. Plasmids 101: Protein Expression

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    Blog Post
    ..., tobacco, rice, sugarcane, and even tubers of potatoes have been used for protein expression. Plant ...well-defined media and can be easily adapted to fermentation allowing for large-scale, stable production ...

  24. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    Blog Post
    ... Base editing, another method to create point mutations, also has a strict editing window about 15 nt ...mutagenesis plasmid (MP6) permits quick, large-scale mutation of the gene of interest. They created a selection...

  25. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...chance of a chromosomal inversion, or just random mutations if the chromosome is repaired with all the original...deleting a problematic copy of a gene that causes a debilitating disease! This is, of course, only hypothetical...

  26. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...eventually found a version of AsLOV2 with five mutations (C450P, L496V, Q513K, G528K, D540M) that shows... and ΔF/F of MagLOV, a variant of AsLOV2 with mutations C450P, L496V, Q513K, G528K, D540M, in response...

  28. Academic vs. Industry Postdocs

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    Blog Post
    ...industry, you’re more likely to give an oral presentation to highlight any new data or ask for additional...industry with most team members giving oral presentations, you will also have to attend many more meetings...

  29. Of Myc and Men

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    Blog Post
    ...partner; as I write this, there are close to 10,000 citations for anti-Myc 9E10 in CiteAb, many of which are...no antibody is that perfect. One of the known limitations was described by Schüchner, et al., who published...

  30. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ... that was not “poised for cleavage”. Jacob computationally added the non-template strand to the Jinek ... this model also displays the several Lys/Ala mutations reported by Ian Slaymaker and colleagues to reduce...
Showing: 601 - 630 of 836 results