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Showing: 601 - 630 of 812 results

  1. Synthetic Photobiology: Optogenetics for E. coli

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    Blog Post
    ...Voigt CA, Ellington AD. Cell. 2009 Jun 26;137(7):1272-81. doi: 10.1016/j.cell.2009.04.048. PubMed. Find...with sophisticated gene expression controls. Most cell types do not respond to low-intensity light, so ...

  2. CRISPR Plasmids - Epigenetics

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    Collection
    ... time and are potentially heritable in dividing cells. CRISPR Activate and Interfere technologies also...

  3. Typing CRISPR Systems

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    Blog Post
    ...III CRISPR-Cas Immunity. Cell, 161(5), 1164–1174. https://doi.org/10.1016/j.cell.2015.04.027 Stella, G.,...and is able to target and edit RNA in mammalian cells. Type IV Type IV is the middle child (like me!) ...-Cas3 for genome-editing applications. Molecular Cell, 84(3), 463-475.e5. https://doi.org/10.1016/j.molcel...biotechnology toolbox for and beyond genome editing. Cell & Bioscience, 8(1). https://doi.org/10.1186/s13578...

  5. Sharing is Caring: But How to Distribute Open Hardware?

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    Blog Post
    ...quantitatively evaluating antibody staining, or counting cells are now routine, open-source software projects that...portable device for studying plankton using flow cells, has become an established alternative to high-priced...

  6. Plasmids 101: The protein expression toolbox

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    Blog Post
    ...force to control protein expression in mammalian cells: easy to use biological tools are abundant! In this...tag prior to introduction of the construct into cells. However, if you would like tunable control of an...

  9. Mouse Modeling, Part 2: Breeding and Crossing Mice

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    Blog Post
    ...strain, the promoter being used should be tested in cell lines to determine how it impacts the expression...interest. Cre may be expressed globally (in every cell of the mouse) or in a tissue-specific manner (with...

  11. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

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    ...annealing to a complementary ssDNA target in the cell.  Only Beta expression is required for recombineering...definitely the easier of the two options, but these cells are prone to mutations and will have more unintended.../Knockin Plasmids to Disrupt Genes in Mammalian Cells CRISPR Methods for Bacterial Genome Engineering ...

  12. CRISPR Plasmids - gRNAs

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    ...is highly recommended that you determine if your cell type contains the target sequence before using any...

  13. CRISPR Plasmids - Cascade-Cas3

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    Collection
    ...bound to DNA, and the break can be repaired by the cell’s endogenous repair mechanisms. Cas3 belongs to the...

  14. Fluorescent Protein Guide: In Vivo Imaging

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    ...stable imaging of protein interactions in living cells and live mice. Proteins for in vivo imaging have...Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Optogenetics Background In vivo ...

  16. The Materials Science of Optogenetics Experiments

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    Blog Post
    ...Pharmacology and Optogenetics. Cell 162.3 (2015): 662-674. doi: 10.1016/j.cell.2015.06.058. Pubmed PMID: 26189679...protein-coupled vertebrate rhodopsin in cerebellar Purkinje cells. The Journal of biological chemistry. 2011;286(29...Derek Simon! Derek P. Simon received his PhD in Cellular and Molecular Biology from the University of Michigan...PMCID: PMC3466483. 4. Kim TI, et al. Injectable, cellular-scale optoelectronics with applications for wireless...

  21. Brzezinski Lab CRISPR Collection

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    Collection
    ...enhancers in the retina alters photoreceptor and bipolar cell fate specification, yet differs from deleting both...

  22. Common Injection Routes in Mice

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    Blog Post
    ...different substances can be injected, including cells, drugs, DNA, viral vectors, and viruses. All researchers... injections are most often used when injecting a cell suspension to create xenograft models. Restraint...

  23. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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    ...screening for functional long noncoding RNAs in human cells by Cas9 targeting of splice sites. Nat Biotechnol...non-coding RNAs with the CRISPR/Cas9 system in human cell lines. Nucleic Acids Research. 2014;43(3):e17. doi...encode proteins. They play important roles in many cellular and regulatory processes, but much of their functionality...

  24. Call for Guest Bloggers

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    Blog Post
    ...Your Favorite Fluorescent Protein Oligomerizes in Cells   ...and many more! Sign up to write for the Addgene...

  26. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...examples could be an endogenous sample of wild type cells, purified protein, or a peptide. The positive control... target antigen, such as a knockout or knockdown cell line or tissue samples where the target is not expressed...

  27. Course-Based Undergraduate Research Experiences (CUREs)

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    Blog Post
    ...identifying new genes required for stem and progenitor cell maintenance in planarians in Developmental Biology...junction complex is required for stem and progenitor cell maintenance in planarians. Developmental Biology... his teaching style, with the former offering excellent training in improvisation and the latter making...

  28. How to Write a Scientific Review Article

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    Blog Post
    ...a review article on histone modifers in B and T cells with my adviser when I was a graduate student. Naturally...acetyltransferases and histone deacetylases in B-and T-cell development, physiology and malignancy." Genes &...

  30. Tips for arabidopsis transformation

    Type
    Blog Post
    ...incubated at stationary phase for too long, the cells in your culture will no longer be viable. The time...graduate student at Stanford University. She studies cell fate maintenance in the stomatal lineage and has...
Showing: 601 - 630 of 812 results