We narrowed to 1,054 results for: STI

...domains from non-vertebrate systems. Preliminary testing indicated that inntags IT5, IT6, and IT10 expressed...and the chaperone Ydj1, both of which display sophisticated interactions with multiple other proteins. Again...

...Bourne has some radical (and some not so radical) suggestions for defining the problem and for finding solutions...don't have the resources to do enough. There are still university research departments that have no formal...

...Structural details All this, and scientists are still working out important details in their understanding... tool. A week later, researchers at the Broad Institute and MIT published another report showing Cas9'...

...new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...CRISPR-based marker excision method for marking distinct cell clones developed by the Michalis Averof lab...

...- increasing open source sharing and helping us stick to our core mission. Chapter 13: Outro - remember...stem cell research as an NIH-funded Principle Investigator in the Kotton Laboratory and is the founding...

...specificity engineering. “The RNA binding domain is interesting because by changing certain amino acids you can...lose their solubility. Her lab is currently investigating the issue and hopes to have a better understanding...

...project involving multiple scientists, create and stick to timelines Be a mentor in a formal program Start...Collaboration and teamwork One of the defining characteristics of nonacademic science careers is that a team...

...individual plasmids to 5,000 different research institutions. This has given us a unique window into technology...plasmid distributed in our 1st year, 2004, and it still gets requested! Fire Lab C. Elegans Kit - Our first...

...anchors for genome engineering applications was still very new. Now, Konermann and her colleagues including...transcription in as little as 30 minutes after light stimulation. Under those optogenetic controls, gene expression...

...new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...of ADAR2 into a cytidine deaminase. This editor still retains A-to-I editing. This system is called RNA...

...our viral service in late 2016 and requests are still pouring in! While our lentiviral service is going... many more tips and ideas on how to use these fantastic tools in your experiments.  Many thanks to Hongkui...

...quality control. Through our partnership with the Institute for Protein Innovation (IPI), we expanded a growing...repository can now be packaged on demand in five distinct serotypes, offering you unparalleled flexibility...

...the Genetic Perturbation Platform at the Broad Institute, John Doench. A genetic screening project can ...at a more-typical scale of tissue culture while still offering the potential for true biological insight...

... in cancer Previously available methods for investigating and functionally validating the thousands of...useful for? They could be used to more thoroughly investigate any of the dozens of wild-type genes and their...

...CHimeric IMmune Editing system) to enable the constitutive, ubiquitous knockout of single genes in immune...To demonstrate the power of these systems to investigate in vivo mechanisms, we targeted two phosphatases...

... FPs since its emission wavelength (612 nm) is distinct from other commonly used FPs, and GFP does not...localization that have been encountered by some investigators when using forms of mEos." The ability to repurpose...

..., designed for use in B. subtilis, contains the MISTIC (membrane-integrating sequence for translation ...integral-membrane protein constructs) protein tag. MISTIC drags tagged proteins into the membrane and may...

... inferred based on correlative biochemical characteristics and ectopic reporter assays. We have been interested...Bauer is a pediatric hematologist and principal investigator at Boston Children’s Hospital and Harvard Medical...

...? This final post in the PI Interview Series investigates how these three PIs reward their team and manage...during the child-rearing years, learning “lots of interesting things from children, their classmates, and parents...

...frequently used pages in your notebooks and go wild with sticky notes and labels. Color code your Post-its and ...write brief descriptions on each. Place the notes sticking up out of the top of the notebooks, so that you...

...localization of individual proteins, but also investigate protein-protein interactions via FRET. Sidae ...partial gene deletion, overexpression (by promoter substitution), and tagging at either the N- or C-terminus...

...resources this way, the CCRP2 is giving Rutgers investigators the agility to rapidly initiate complex research... As researchers around the world continue to investigate COVID-19, and once universities reopen and restart...

...months of strategizing, designing, developing, testing - and repeat - we’re proud and excited to finally... than through the ‘front door,’ the homepage is still important. It needs to express value, clearly communicate...

...sought to support scientists at all research institutions. In 2012, we shipped our first plasmid to an... Laboratory. Through our partnership with the Institute for Protein Innovation, Addgene also distributes...

...recombination at pseudo- or cryptic loxP sites (estimated to occur at a frequency of 1.2 per megabase in...toxicity of this enzyme in Drosophila. If you're still reading this post and you don't work in Drosophila...

...impressive 1,350 scientists from more than 275 institutions all over the world. In the course of all those...categories: Over 300 empty backbones with 100+ distinct fluorophores to choose from in the green, red,...

...analysis, Tm and primer dimer estimation, and other primer characteristics. NEB Data-optimized Assembly...

...process known as phagocytosis.  Other functions We’re still learning about Fc effector functions and all that...driving particular cells or responses. And there are still antibody isotypes and Fc receptors that we don’t...

...sometimes even genetic pathways that are worth investigating for drug development. Due to the difficulty ... to determine if a patient would respond to an existing therapy. In theory, CRISPR could even be used ...

...neutralize the nuclease activity. For strains that still carry this gene, there are usually extra steps like...the pH preparation stabilizing the plasmid, and still allowing most downstream processing. On the other...

...two gRNAs.  For nickase applications, a common question is: how should the gRNAs be oriented in comparison...insertions. These profiles are likely due to the distinct overhang patterns generated by the nickases; D10A...

... we can predict the resulting band sizes when digesting the plasmid sequence with one or more enzymes....Reference Pages Basic Molecular Biology Protocols Diagnostic Restriction Digest Protocol ...

...publications in 2025 alone, each representing questions answered, hypotheses tested, and knowledge advanced...locate exactly the right tools for your experiments. Still others told us about the need for more open-source...

... but boy does it have a lot of parts! If you’re still a bit confused by CRISPR acronyms, this post is ...appears between each spacer in the CRISPR array, distinguishing the foreign sequences and keeping the array...

...I just keep learning on the job. It keeps it interesting.” And while his official title may be science... his boss was looking for someone to take over hosting them. Since then, webinars make up a larger part...

...for new ways to serve the research community. Questions? Feel free to reach out to us on Twitter, Facebook... Ishrat, Jane, Jason, Jessica, Joel, Jonathan, Justine, Karen, Kate, Kayla, Kelly, Kory, Kris, Leila, ...

...has “literally enabled sharing with 100's of investigators worldwide. Now everything from every paper is... Addgene is that we cover all of the shipping logistics and facilitate all material transfer agreements...

... my kids like any other youngster. His parents, Kristi and Rico, had to keep him from running too much...the fight against FOP. Second, Addgene will be hosting the AJ’s Boston League team (Contributing many ...

...CRISPR screens symposia outlined the fantastic work this Broad Institute lab is doing creating well-optimized...

... I've been answering biology customer service questions for nearly 7 years, and my colleagues and I have... worked so hard to make this poster, offered suggestions and tips, and helped get me to this meeting. ...