We narrowed to 1,092 results for: addgene
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TypeBlog Post...Resources and references Additional resources on the Addgene blog Mouse Modeling Part 1 Mouse Modeling Part ...Popular Model Organisms Additional resources on Addgene.org Genome Engineering Guide CRISPR Mammalian Expression...
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Plasmids 101: Degron Tags
TypeBlog Post...Gene Tagging Plasmids 101 eBook Resources on the Addgene blog Plasmids 101: Protein Tags Plasmids for Endogenous...doi.org/10.1073/pnas.1216880109 Resources on Addgene.org CRISPR Plasmids – Protein Tagging CRISPaint ... -
Viral Vectors 101: Chemogenetics
TypeBlog Post...amenable to clinical Additional resources on the Addgene blog New to viral vectors? Browse our Viral Vectors... blog posts about neuroscience Resources on addgene.org Learn more about chemogenetics in our chemogenetics... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog Post...find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources... Various Addgenies... -
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine
TypeBlog Post...PMCID: PMC3347549. Additional Resources on the Addgene Blog pCXLE Toolkit Stem Cell Models for Disease...Differentiate the Stem Cell Field Resources on Addgene.org Visit Our Stem Cell Page Find Plasmids for Reprogramming... -
Antibodies 101: Flow Compensation
TypeBlog Post...Harvard Medical School. More resources on the Addgene blog Introduction to Gating in Flow Cytometry Antibodies... -
Rabies and Neuronal Tracing
TypeBlog Post...Pubmed PMID 17179932. Additional Resources on the Addgene Blog AAVs CREATed for Gene Delivery to the CNS...Gene Expression with FLEx Vectors Resources on Addgene.org AAV Vector for TVA Expression AAV for Rabies... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog Post...PMCID: PMC4889673. Additional Resources on the Addgene Blog Learn about Fluorescent Protein Timers Check...Use Rosella to Monitor Autophagy Resources on Addgene.org Check out the SapTrap Kit Page Find Other Fluorescent... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog Post.../2021.11.01.466786 Additional Resources on the Addgene Blog Prime Editing: Adding Precision and Flexibility... With Cas13 CRISPR Cheat Sheet Resources at Addgene.org CRISPR Guide... -
What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry
TypeBlog Post... Tech Tip (2013). Additional resources on the Addgene blog Plasmid Preps: Different Purity, Different... Your Plasmid Purification Kit Resources on Addgene.org DNA Quantification Protocol Recovering Plasmid... -
Plasmids 101: Shuttle Vectors
TypeBlog Post...vectors a success! Resources Resources on the Addgene blog Plasmids 101: Transformation, Transduction...Plasmids 101: The Promoter Region Resources on Addgene.org Collection of empty plasmid backbones ... -
Degrading DNA with Cascade-Cas3
TypeBlog Post...crime, Cascade. Find Cascade-Cas3 plasmids in Addgene's collection! A "complex" system Cas3 is part of... CRISPR-Transposons Additional resources on Addgene.org CRISPR guide Cascade-Cas3 Plasmid collection... -
Isolating Genomic Regions of Interest with the CRISPR System
TypeBlog Post...PMCID: PMC5470959. Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by... -
Getting to Know Expansion Microscopy
TypeBlog Post....7554/elife.46249 Additional resources on the Addgene blog Antibodies 101: Introduction to Immunofluorescence... -
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons
TypeBlog Post.../acssynbio.0c00073 Additional resources on the Addgene blog Browse all of our CRISPR blog posts Check...other CRISPR methods for bacteria Resources on Addgene.org Visit the CRISPR guide Find CRISPR plasmids ... -
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More
TypeBlog Post...(2012): 4099-4103. Additional resources on the Addgene blog Read about zebrafish behavioral studies Download...Read our plasmids 101 blog series Resources on Addgene.org Browse our zebrafish CRISPR plasmid collection... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog Post...PubMed PMID: 29335603. Additional resources on the Addgene blog Browse our CRISPR blog posts Download our...sheet Download our CRISPR eBook Resources on Addgene.org Check out our comprehensive CRISPR guide Find... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog Post...PMCID: PMC4511399. Additional resources on the Addgene blog Learn more about CcdB, the toxic key to efficient...about knockout/knock-in plasmids Resources on Addgene.org Check out our molecular biology reference Search... -
Simplify Cloning with in vivo Assembly
TypeBlog Post...PMCID: PMC4893743. Additional resources on the Addgene blog Read all of our Plasmids 101 blog posts Read... blog posts on plasmid cloning Resources on Addgene.org Read our protocols for plasmid cloning Find ... -
Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity
TypeBlog Post...PMCID: PMC6061786. Additional resources on the Addgene blog Learn the basics of Golden Gate assembly ...other plasmid cloning techniques Resources on Addgene.org Find basic molecular biology protocols Find ... -
AAVs in Retinal Gene Therapy
TypeBlog Post...PubMed PMID: 26958729. Additional Resources on the Addgene Blog Adeno-Associated Virus (AAV) for cell and...for gene expression in mammals Resources at Addgene.org Find AAV plasmids Find ready-to-use AAV viral... -
Magnetic Control of Proteins: More than a Dream
TypeBlog Post... al., 2024). They deposited their plasmid with Addgene, hoping to inspire others to explore the phenomenon...doi.org/10.1038/nn.4265. Additional resources on addgene.org Find Optogenetics Plasmids Find Biosensor Plasmids... -
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog Post...j.cell.2023.03.034 Resources More resources on the Addgene blog How to Design Your gRNA for CRISPR Genome ...and Flexibility to CRISPR Editing Resources on Addgene.org CRISPR: PRIME Edit Collection The CRISPR Guide... -
Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog Post...in the real world. Additional Resources on the Addgene Blog Browse our CRISPR featured topic page xCas9... Editing with Cas13 and REPAIR Resources on Addgene.org Check out our CRISPR guide Find CRISPR plasmids... -
Fluorescent Proteins 101: Fluorescent Protein Timers
TypeBlog Post...PMCID: PMC4831023. Additional Resources on the Addgene Blog Learn More About Fluorescent Protein Aggregation...Choosing Your Fluorescent Protein Resources at Addgene.org Find Fluorescent Protein Timers Browse All Fluorescent... -
How to Write a Scientific Review Article
TypeBlog Post...PMCID: PMC4482241. Additional Resources on the Addgene Blog Writing Scientific Manuscripts: Literature...Manuscript 6 Tips for Grant Writing Resources on Addgene.org Check out Our Hot Plasmids Articles to See Compiled... -
Better Dyeing Through Chemistry & Small Molecule Fluorophores
TypeBlog Post...PMCID: PMC3246539. Additional Resources on the Addgene Blog Learn About Other Protein Tags Catch Up on...Fluorescent Protein Should I Use? Resources on Addgene.org Generate Fluorescent Protein Fusions Request... -
Experimenting with New Careers while in Grad School
TypeBlog Post...more people to quit the lab. Resources on the Addgene Blog Read Joanne Kamen's Blog Series on Mentoring...Browse All of Our Career Posts Resources on the Addgene.org Find Plasmids for Your Research Learn About ... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog Post... Pubmed Central PMCID: PMC514463. Resources At Addgene Browse Our Yeast Expression Vectors Find Bacterial... -
A Career in Grants: How To Become a Grant Professional
TypeBlog Post...Additional Resources Read other Career Posts on the Addgene Blog! ... -
Light Sheet Fluorescence Microscopy
TypeBlog Post...). These viral plasmids are available through Addgene. The combination of LSFM with multiple tissue ... -
Plasmids 101: Control Plasmids
TypeBlog Post...PubMed Central PMCID: PMC3321166. Resources on the Addgene Blog Origin of Replication The Promoter Region... -
Plasmids 101: The Promoter Region – Let's Go!
TypeBlog Post...writing of this article. Additional Resources on the Addgene Blog Browse All Plasmids 101 Posts Learn about...Reporter Gene like Luciferase and GFP Resources on Addgene.org Find Plasmids for Your Research ... -
CRISPR 101: Cytosine and Adenine Base Editors
TypeBlog Post...activity. Find CRISPR base editing plasmids at Addgene Improving cytosine base editing scope and efficiency.../s41587-020-0527-y Additional Resources on the Addgene Blog CRISPR 101: Homology-Directed Repair CRISPR...base editor reporters Additional Resources on Addgene.org Catch up on your CRISPR background with our ... -
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time
TypeBlog Post...10.1093/nar/gkz713 Additional resources on the Addgene blog Read about other CRISPR reporters Browse ...Browse our CRISPR blog posts Resources on Addgene.org Browse the CRISPR guide Find CRISPR plasmids for your... -
Antibodies 101: Reading a Flow Plot
TypeBlog Post...10.1371/journal.pone.0137550 More resources on the Addgene blog Conventional vs. Flow Cytometry Plasmid-based... -
CRISPR 101: Homology Directed Repair
TypeBlog Post... (2017). PubMed. Additional Resources on the Addgene Blog Check out our CRISPR Featured Topic Page...CRISPR Editing in Human Cells Resources on Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog Post...j.cell.2020.03.023 Additional Resources on the Addgene Blog Learn more about Cas9 Variants and the Expanded...Cpf1 Read our Introduction to AAV Resources on Addgene.org Check out our CRISPR Guide Find CRISPR Plasmids... -
Plasmids 101: Stringent Regulation of Replication
TypeBlog Post.../doi.org/10.1093/emboj/20.24.7323 Resources at Addgene Read our blog post on the Origin of Replication... -
Adeno-associated Viruses (AAVs) for Genome Editing
TypeBlog Post...PMCID: PMC5702533. Additional Resources on the Addgene Blog AAV: A Versatile Tool for Gene Expression...Made in Heaven: CRISPR and AAV Resources on Addgene.org Check out our Viral Service Find AAV Vectors... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog Post...PMCID: PMC4760889. Additional Resources on the Addgene Blog Learn More about FLEX-vectors Learn More ... AAVs for Neurscience Research Resources on Addgene.org Find Cre-Lox Plasmids Find Optogenetics Plasmids... -
Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog Post...PMCID: PMC3524131. Additional Resources on the Addgene Blog Use Site Directed Mutagenesis to Generate...Mutagenesis to Mutate Your Plasmids Resources on Addgene.org Find Plasmids from the Whitehead Lab Use CRISPR... -
Site Directed Mutagenesis by PCR
TypeBlog Post...PubMed Central PMCID: PMC4333370. Resources on the Addgene Blog Brush up on Your Cloning Techniques Learn...Generate Specific Genomic Mutations Resources on Addgene.org Browse Our Molecular Biology Protocols Find ... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog Post...PubMed Central PMCID: PMC3032981. Resources at Addgene A Primer on Optogenetics: Introduction and Opsin... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog Post...Additional Resources Find other career posts on the Addgene Blog! Learn more about Experimental Designs Consulting... -
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
TypeBlog Post...PMCID: PMC4723041. Additional resources on the Addgene blog Read our fluorescent proteins blog posts ...Read our plasmids 101 blog series Resources on Addgene.org Browse the fluorescent protein collection Find... -
New Optogenetic Tools for Cytoskeleton and Membrane Control
TypeBlog Post...resources on the Addgene blog Viral Vectors 101: Optogenetics Additional resources on Addgene.org Optogenetics... -
Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog Post...PubMed PMID: 24733238. Additional resources on the Addgene blog Read our Plasmids 101 blog posts Find our...about Lambda Red recombineering Resources on Addgene.org Find plasmids for allelic exchange Read our ... -
Quick Guide to Near-Infrared Fluorescent Proteins
TypeBlog Post...-the-art probes, many of which you can find at Addgene. Biliverdin chromophore Near-infrared fluorescent... Ref BphP-based FPs miRFP670 https://www.addgene.org/79987/ 642 670 87,400 14.0 198 Monomer 155...155 4.5 72 5 miRFP703 https://www.addgene.org/79988/ 674 703 90,900 8.6 127 Monomer 394 4.5 37 ... miRFP709 https://www.addgene.org/79989/ 683 709 78,400 5.4 69 Monomer 192 4.5 30 mIFP https://www.addgene.org...112 Monomer 54 4.5 15 15 miRFP https://www.addgene.org/108409/ 674 703 92,400 9.7 145 Monomer ND ...ND 4.3 ND 16 IFP2.0 e https://www.addgene.org/54785/ 688 709 98,000 8.1 128 Dimer 108 4.5 8 17 ... iRFP670 https://www.addgene.org/45457/ 643 670 114,000 12.2 225 Dimer 290 4.0 119 18 iRFP682 https... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog Post...PMCID: PMC5837189. Additional Resources on the Addgene Blog Read our Introduction to GFP Read our Fluorescent...Fluorescent Proteins 101 eBook Resources on Addgene.org Browse the Fluorescent Protein Collection Find...