We narrowed to 724 results for: ACE
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TypeBlog Post... to undergraduate students (1, 2), but educators face significant challenges in designing suitable projects...complementation should only occur when galactose replaces glucose as the carbon source. Student results ...
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Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog Post...from the drop-down and add an explanation in the adjacent Patent/License Explanation field. Filling ...in the format: labname_addgene_batch_upload.xls Replace ‘labname’ with the name of your PI and send the... -
6 Tips for Grant Writing
TypeBlog Post...co-founder of www.assaygenie.com. No matter what facet of academia you are in, grant writing can be the...clear that you are staying because it is the best place in the world for your research to rapidly progress... -
Tips for Technical Support Calls
TypeBlog Post...Google it...Or Yahoo! it, or Bing it, but, let’s face it, there’s a reason nearly 70% of all internet ...experiment, but a picture of a gel or a sequencing trace file can go a long way in helping us understand ... -
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015
TypeBlog Post...gene cassettes. Thse vectors are engineered to replace the drug resistantance genes in the bacteria thus...known antibotics. These might be used on hospital surfaces and in hand sanitizers. There are many clever... -
The Materials Science of Optogenetics Experiments
TypeBlog Post... scatters a great deal once it enters tissue, placement above the site of stimulation guarantees that ...brain tissue a short distance so ideal implant placement should be about 0.4-0.5mm above the site of your... -
Gaining Leadership Skills Volunteering at a Professional Organization
TypeBlog Post...teamwork, manage day to day operations within their workplace, and pursue leadership opportunities at the local...postdoc). I soon realized that many of the issues faced by postdocs and institutions are not local but are... -
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)
TypeBlog Post...conferences, but after receiving her degree she was faced with a similar conundrum – she had the skills, training...color changing flower project was inspired by a Facebook comment. In our day job, we worked on plants ... -
Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR
TypeBlog Post...that are distant linearly but close together in space. The chromatin “picture” is then developed by detecting...the following changes to create CAPTURE 2.0: Replace the biotinalatable tag on dCas9 with a BioTAP-tag... -
The Scientific Conference Poster Session: Tips for Success
TypeBlog Post..., but those outside of your field can point out places where you gloss over essential details or omit ...avoid rushing out the door without your poster, place it by your keys a day in advance. Dress for the ... -
Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity
TypeBlog Post...England Biolabs. Golden gate assembly limitations Embraced by the synthetic biology community, Golden Gate...efficiency, stability, and buffer compatibility have placed practical limits on single- or two-step assemblies... -
Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog Post...so many technologies currently available, you now face the challenge of trying to figure out what system...Scientific Inc., Origene, and NEB® are all good places to look for some helpful kits! Efficiency: Wouldn... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog Post...track protein localization, cell structure, intracellular trafficking, and protein turnover rates. Additionally...overexpressed fluorescent protein fusions is to replace the genomic copy of your gene of interest with ... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog Post...is typically purified using methods like ultracentrifugation, while lentivirus supernatants are centrifuged...collection 2–4 days 5–10 days Purification Ultracentrifugation (AAV); centrifugation (lenti) Centrifugation... -
Magnetic Control of Proteins: More than a Dream
TypeBlog Post...applications across a wide range of topics. He embraces open science, self-publishing directly on his .... R., Evans, E. W., Déjean, V., Dodson, C. A., Wallace, M. I., Mackenzie, S. R., Timmel, C. R., & Hore... -
PhD Applications After COVID
TypeBlog Post...simply as it seems laid out above, there are many places where the PhD application cycle becomes much more...program will allow for you to have the time and space to delve into what matters to you. It can also be... -
Transferable Skills Guide: Managing a Team
TypeBlog Post...comfortable with awkward silences but I’ve learned to embrace them after one ended with the person in front of...your manager for advice if you’re not sure how to face a particular situation. But keep in mind that a ... -
Tips for Using BLAST to Verify Plasmids
TypeBlog Post... has refined our use of the BLAST web browser interface to be as efficient as possible. If you find yourself...longer alignments to compare with your sequencing trace file. Unlike megablast, the regular blastn program... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog Post...individual plasmid orders, because when a PI or lab places an order, they are likely to order several plasmids...next-generation therapeutics. Prime editing, base editing, PACE, and DNA-templated synthesis are four examples of... -
A Guide to Starting Your Own Journal Club
TypeBlog Post... journal club? Where will the journal club take place? Preparing for the first meeting There are many ...important pieces before that first meeting takes place to ensure success. One of the most important is ...